SSE15206-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESSE15206Cat. No.: HY-111425CAS No.: 1370046-40-4分式: CHNOS分量: 371.45作靶点: Microtubule/Tubulin作通路: Cell Cycle/DNA Damage; Cytoskeleton储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 150 mg/mL

2、(403.82 mM; Need ultrasonic)H2O : 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (6.73 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (6.73 mM); Suspended solution; Need ultrasonic3. 请依序添加每种溶剂： 10% DMSO 90% corn oil1/2 Master of Small Molecules 您边的抑

3、制剂师www.MedChemESolubility: 2.5 mg/mL (6.73 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 SSE15206种微管 (microtubule) 聚合抑制剂，其在 HCT116 细胞中的 GI50 值为 197 nM，能克服多重 耐药性。引起细胞中纺锤体形成不完全，从导致 G2/M 阻滞，有丝分裂异常。IC50 & Target GI50: 197 nM (microtubule) 1.体外研究 SSE15206 induces apoptosis in cells irrespective of MDR-1 over

4、expression cell lines (KB-V1, A2780-Pac-Res), highly resistant to paclitaxel cells (HCT116-Pac-Res) and parental cells at the concentration of 5 and10 GI50 values. To conclude, SSE15206 is able to overcome resistance to chemotherapeutic drugs suchas paclitaxel in different cancer cell lines 1.Wester

5、n Blot Analysis 1Cell Line: Drug-resistant cell lines (KB-V1, A2780-Pac-Res, HCT116-Pac-Res) and parental cells.Concentration: 5 and 10 GI50 values (GI50=197 nM in HCT-116 cells).Incubation Time: 24 hours.Result: Induced apoptosis in cells irrespective of MDR-1 overexpression and HCT116-Pac-Resand p

6、arental cells.REFERENCES1. Manzoor S, et al. Identification and characterization of SSE15206, a microtubule depolymerizing agent that overcomes multidrugresistance. Sci Rep. 2018 Feb 19;8(1):3305.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400-820