Glucoraphanin - Reactive Oxygen Species - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEGlucoraphaninCat. No.: HY-N4068CAS No.: 21414-41-5分式: CHNOS分量: 437.51作靶点: Reactive Oxygen Species作通路: Immunology/Inflammation; Metabolic Enzyme/Protease; NF-B储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month

2、溶解性数据体外实验 H2O : 76.5 mg/mL (174.85 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.2857 mL 11.4283 mL 22.8566 mL5 mM 0.4571 mL 2.2857 mL 4.5713 mL10 mM 0.2286 mL 1.1428 mL 2.2857 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Gluc

3、oraphanin存在于字花科蔬菜中的硫代葡萄糖苷，是Nrf2 诱导剂 sulforaphane 的前体，后者具有抗氧化、抗炎、抗癌的作。体内研究Glucoraphanin reduces weight gain and increases energy expenditure in HFD-fed mice. Glucoraphanin canimproves insulin sensitivity and glucose tolerance in HFD-fed mice. However, Glucoraphanin does not exertantiobesity and insul

4、in-sensitizing effects in Nrf2/ Mice. Glucoraphanin blocks HFD-induced reduction of1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEUcp1 protein levels in white adipose depots of wild-type mice but not in Nrf2/ mice. Glucoraphaninalleviates HFD-induced hepatic steatosis and oxidative stress. Glucora

5、phanin suppresses HFD-inducedproinflammatory activation of macrophages in liver and adipose tissue. Glucoraphanin also decreasescirculating LPS and the relative abundance of proteobacteria in the gut microbiomes of HFD-fed mice 1.Mice with pellets including 0.1% Glucoraphanin (GF) significantly atte

6、nuates the decreased social avoidancetime in stressed mice. In the 1% sucrose preference test (SPT), treatment with pellets including 0.1% GFsignificantly attenuates the decreased sucrose preference of stressed mice 2.PROTOCOLAnimal Male C57BL/6JSlc mice are 7 weeks of age. The Nrf2 knockout (Nrf2/)

7、 mouse strain is used in the assay.Administration 1 After 1 week of acclimation, mice are fed normal chow (NC) (containing 2.2% dextrinized cornstarch, 10%kcal from fat), NC containing 0.3% glucoraphanin (NC-GR) (containing 2.2% extract powder), an HFD(containing 2.2% dextrinized cornstarch, 60% kca

8、l from fat), or an HFD containing 0.3% glucoraphanin (HFD-GR) (containing 2.2% extract powder) for 14 weeks. Both the NC and the HFD containing cornstarch orglucoraphanin are prepared. All mice studied are maintained on a 12-h light/dark cycle at 24-26C with freeaccess to water and food.MCE has not

9、independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Nagata N, et al. Glucoraphanin Ameliorates Obesity and Insulin Resistance Through Adipose Tissue Browning and Reduction ofMetabolic Endotoxemia in Mice. Diabetes. 2017 May;66(5):1222-1236.2. Yao W, et al. Role of Keap1-Nrf2 signaling in depression and dietary intake of glucoraphanin confers stress resilience in mice. Sci Rep.2016 Jul 29;6:30659.McePdfHeightCaution: Product has not been fully validated fo