Allantoin-5-Ureidohydantoin-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEAllantoinCat. No.: HY-N0543CAS No.: 97-59-6Synonyms: 5-Ureidohydantoin分式: CHNO分量: 158.12作靶点: Imidazoline Receptor; Endogenous Metabolite作通路: GPCR/G Protein; Neuronal Signaling; MetabolicEnzyme/Protease储存式: Powder -20C 3 years4C

2、2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 6 mg/mL (37.95 mM; Need ultrasonic and warming)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 6.3243 mL 31.6216 mL 63.2431 mL5 mM 1.2649 mL 6.3243 mL 12.6486 mL10 mM 0.6324 mL 3.1622 mL 6.3243 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和

3、期限。BIOLOGICAL ACTIVITY物活性 Allantoin促进肤健康，刺激新鲜健康组织长的肤调理剂。IC50 & Target Human Endogenous Metabolite体外研究Allantoin is a well-known cosmetic ingredient reported to have anti-oxidative and anti-inflammatory activities1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE1. Allantoin attenuates apoptosis and cy

4、totoxicity and increased the viability of STZ-induced -cells in adose-dependent manner. Allantoin decreases the level of caspase-3 and increases the level ofphosphorylated B-cell lymphoma 2 (Bcl-2) expression. Allantoin has been demonstrated to activateimidazoline 3 (I3) receptors 2.体内研究 The subchro

5、nic administration of allantoin (1, 3 or 10 mg/kg, for 7 days) significantly increases the latencytime measured during the passive avoidance task in scopolamine-induced cholinergic blockade and normalnaive mice. Allantoin treatment (3 or 10 mg/kg, for 7 days) also increases the expression levels ofp

6、hosphorylated phosphatidylinositide 3-kinase (PI3K), phosphorylated protein kinase B (Akt) andphosphorylated glycogen synthase kinase-3 (GSK-3). Allantoin significantly increases the neuronal cellproliferation of immature neurons in the hippocampal dentate gyrus region 1. Daily injection of allantoi

7、n for 8days in STZ-treated rats significantly lowers plasma glucose and increases plasma insulin levels 2. Allantoindecreases blood pressures in SHRs at 30 minutes, as the most effective time. Also, this antihypertensiveaction is shown in a dose-dependent manner from SHRs treated with allantoin. Mor

8、eover, in anesthetizedrats, allantoin inhibits cardiac contractility and heart rate. Also, the peripheral blood flow is markedlyincreased by allantoin 3.PROTOCOLCell Assay 2 Pancreatic -cells are treated with 1, 10, 100 M of allantoin before 30 min prior to the addition of 5 mM STZand incubated for

9、6 h. Cell viability is measured using the ApoTox-Glo triplex assay 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Rats: Animals are randomly assigned into four groups: (I) the control group treated with the vehicle, saline;Administration 13 (I

10、I) the allantoin group treated by intravenous injection of allantoin at 0.5mg/kg; (III) the allantoin+efaroxangroup treated with allantoin at the most effective dose (0.5mg/kg, i.v.) and efaroxan at effective dose (1.5mg/kg, i.v.) 30 minutes before injection of allantoin; and (IV) the allantoin trea

11、ted SHRs group treated byintravenous injection of allantoin at various dose for desired time. After treatment of allantoin, the rats areplaced into a holder for the determination of the mean blood pressure 3.Mice: For memory ameliorating study, mice are administered vehicle solution, allantoin (1, 3

12、 or 10 mg/kg,p.o.) or donepezil (5 mg/kg, p.o.) at the same time (10:00-12:00 a.m) and same place for 7 days. For memoryenhancing study, mice are administered vehicle solution, allantoin (1, 3 or 10 mg/kg, p.o.) or piracetam (200mg/kg, i.p.). The final administration of allantoin, donepezil or pirac

13、etam is performed 1 h before anacquisition trial in the passive avoidance task 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Ahn YJ, et al. Effects of allantoin on cognitive function and hippocampal neurogenesis. Food Chem Toxicol. 2014

14、 Feb;64:210-6.2. Amitani M, et al. Allantoin ameliorates chemically-induced pancreatic -cell damage through activation of the imidazoline I3 receptors.PeerJ. 2015 Aug 6;3:e1105.3. Chen MF, et al. Antihypertensive action of allantoin in animals. Biomed Res Int. 2014;2014:690135.McePdfHeight2/2 Master of Small Molecule