EB病毒相关胃癌论文：EB病毒相关胃癌HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3病理 临床预后

1、最新EB病毒相关胃癌论文：EB病毒相关胃癌HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3病理 临床预后【关键词】EB病毒相关胃癌 HER2 基因扩增 蛋白表达 胃癌 表皮生长因子受体家族 HER3 病理 临床 预后【英文关键词】Epstein-Barr virus associated gastric carcinoma HER2 geneamplification protein expressiongastric cancer HER family HER3 pathology clinicalEB病毒相关胃癌论文：胃癌中HER2基因扩增及HER家族蛋白表达的临床病理意

2、义【中文摘要】本课题分为以下二局部：第一局部EB病毒相关胃癌中HER2蛋白表达及基因扩增检测EB病毒相关胃癌EBVaGC与EB病毒非相关胃癌EBVnGC中HER2蛋白表达及基因扩增情况，初步探讨它们在两种胃癌中差异的原因。1运用免疫组化方法检测EBVaGC及EBVnGC中HER2蛋白表达水平，分析两种胃癌中HER2蛋白表达率及过表达率的差异。2采用荧光原位杂交方法检测EBVaGC及EBVnG中HER2基因有无扩增，分析两种胃癌中HER2基因扩增率的差异。1EBVaGC及EBVnGC中HER2蛋白均存在不同程度的表达及过表达，EBVaGC中HER2蛋白表达率及过表达率分别为24.4%11/45及

3、6.67%3/45，EBVnGC中HER2蛋白表达率及过表达率分别为68%34/50及28%14/50，两种胃癌HER2蛋白表达率及过表达率均有显著差异P0.001，P=0.007。2EBVaGC及EBVnGC中均存在HER2基因扩增，EBVaGC中HER2基因扩增率为2.2%1/45, EBVnGC中HER2基因扩增率为18%9/50，二者有显著差异P=0.03。3在EBVaGC及EBVnGC中，HER2免疫组化染色结果为0和1+的组织HER2基因均无扩增，HER2免疫组化染色结果为3+的组织HER2基因均有扩增。EBVaGC组织中HER2蛋白表达率、过表达率及HER2基因扩增率较EBVnG

4、C组织均显著降低。第二局部胃癌中HER家族蛋白表达及过表达的临床病理意义通过检测人类表皮生长因子受体家族HERs蛋白在胃癌组织中的表达，探讨HER家族蛋白表达、过表达、联合表达及联合过表达与胃癌临床病理特征及预后之间的关系。采用免疫组织化学法检测124例手术切除的胃癌组织中HER家族蛋白的表达，分析HER家族蛋白表达、过表达、联合表达及联合过表达与胃癌临床病理特征和预后之间的关系。1124例胃癌中HER家族蛋白HER1、HER2、HER3、HER4的表达率分别为41.1%51/124、59.7%74/124、82.2%102/124、28.2%35/124；其过表达率分别为6.5%8/124、

5、26.6%33/124、22.6%28/124、12.1%15/124；癌旁非肿瘤胃粘膜均未见HER家族蛋白表达。2肠型胃癌中HRE2蛋白过表达较弥漫型胃癌更常见P=0.001。3HER3过表达的胃癌常浸润深度更深、淋巴结转移、远处转移、TNM分期更晚及预后更差。P均0.05；HER1、HER2和HER3联合表达与胃癌远处转移有关P=0.035。HER家族中HER3过表达可以作为评估胃癌恶性生物学行为的指标。【英文摘要】The paper includes two parts as follows:The first partHER2protein expression and HER2gen

6、e amplification in EBVaGC【s】To detect the expression of HER2protein and HER2gene copy number inEBVaGC and EBVnGC, and discuss the diffirences between these two kinds ofgastric carcinoma.【Methods】1. The expression of HER2protein and HER2gene copy number weredetermined by immunohistochemistry and FSIH

7、 in45cases of EBVaGC and50casesof EBVnGC.2. To discuss the differences of rates of HER2protein expression, overexpressionand HER2gene amplification between EBVaGC and EBVnGC. 【Results】1. In EBVaGC,the rates of HER2protein expression and overexpression were24.4%(11/45) and6.67%(3/45), respectively; I

8、n EBVnGC, the rates of HER2proteinexpression and overexpression were68%(34/50) and28%(14/50), respectively; therates of HER2protein expression and overexpression were obviousely decreased inEBVaGC (P 0.001, P=0.007).2. HER2gene was amplified in EBVaGC and EBVnGC, the rates of HER2geneamplification w

9、ere2.2%(1/45) and18%(9/50), respectively. The rates of HER2geneamplification were obviousely decreased in EBVaGC (P=0.007).3. In EBVaGC and EBVnGC, all the grade of “0 and “1+ in IHC were negativein FISH, and all the grade of “3+ in IHC were positive in FISH.【Conclusions】The rates of HER2protein exp

10、ression, overexpression and HER2geneamplification were obviousely decreasede in EBVaGC. The second partThe clinicalpathological significance of expression andoverexpression of HER family proteins in gastric carcinoma【s】To investigate the correlation of expression, overexpression, co-expression andco

11、-overexpression of HER family proteins with the clinicopathological parameters ofgastric cancer.【Methods】Expression of HER1-4proteins were determined by immunohistochemistry in124gastric cancer tissues, the correlation of expression, overexpression,co-expression and co-overexpression of HER family p

12、roteins with clinicopathological parameters and prognosis of the patients were also analyzed.【Results】1. The expression rates of HER family proteins in124gastric cancer were41.1%(51/124),59.7%(74/124),82.2%(102/124) and28.2%(35/124), respectively.2. The overexpression rates of HER1-4proteins in the

13、patients with gastriccancer were6.5%(8/124),26.6%(33/124),22.6%(28/124), and12.1%(15/124),respectively.3. HER2overexpression was associated with intestinal-type gastric cancer(P=0.001).4. HER3overexpression was associated with depth of invasion, lymph nodemetastasis, distant metastasis, TNM stage of

14、 gastric cancer and worse survival(P0.05); the co-expression of HER1, HER2and HER3were associated with distantmetastasis of gastric cancer (P=0.035).【Conclusions】Overexpression of HER3may be used to assess the malignant biologicalbehavior and prognosis of gastric cancer.【目录】胃癌中HER2基因扩增及HER家族蛋白表达的临床病理意义中文摘要3-6ABSTRACT6-8目录9-10第一局