发育生物学讨论课topics pre

1、Main paperWANG YuchunLIU QingContents2IntroductionWANG MinmingDiscussionGENG QiIntroduction1Main Paper2Parietal cells(secrete HCl)Chief cells(secrete pepsinogen)Gastric mucosa(secretes gastrin)Fundus Corpus Antrum 王闵铭 你会介绍如何培养到endoderm时期吗？K.M. McCracken et al., Nature,2014WNT and FGF without BMP dri

2、ve morphogenesis of gut tube structuresTae-Hee Kim et al.,Development, 2016WNT and FGF without BMP drive morphogenesis of gut tube structuresWNT, FGF and BMP cooperate to promote a mid-hindgut fate, but WNT and FGF act without BMP to drive morphogenesis of gut tube structures.K.M. McCracken et al.,

3、Nature,2014Retinoic acid signaling acts for patterning of foregutK.M. McCracken et al., Nature,2014Additional retinoic acid signaling specifies gastric antrum fateEGF promote hGO formationK.M. McCracken et al., Nature,2014LandmarksHoweverThe first human gastric tissue fully derived in vitro. A robus

4、t system to study antral lineage allocation and hostmicrobe interactions in the stomach No fundus structure emerged during culture. Do not allow studies of fundic biology and disease. What and How to do next?But I know also that still more interesting discoveries will be made that I have not the ima

5、gination to describe - and I am awaiting them, full of curiosity and enthusiasm.- LInus PaulingWhat causes fundus specification?Different molecular markersDifferent gene expression levelPathway regulationThe embryonic fundus domain is Sox2+ Gata4+ Pdx1Irx2, Irx3, Irx5 are highly expressed in fundusM

6、icroarray data Fundic and antral domains are established by E10.5Meanwhile, different Wnt signaling activities are observedThe canonical Wnt signalling pathway was active in the proximal stomach while exhibited little or no activity in the distal stomachWhat regulates fundus specification?Loss of fu

7、nction testGain of function testDisturbance of molecular marker expressionReduced gene expression levelHistologic and cytological changesWnt signalling was required for establishing the fundusDisruption of Wnt signaling lead to ectopic Pdx1 expression loss of fundic identityDisruption of Wnt signali

8、ng reduce the expression of Irx2, Irx3 and Irx5.Wnt signalling was required for establishing the fundusWnt signalling was required for establishing the fundusAtp4b Pdx1 Cdh1The stomach in cKO embryos was malformed and reduced in size at E18.5.(also with Pdx1 ectopic expression)Wnt signalling was required for establishing the fundusParietal cells were completely absent in-catenin-deficient epitheliumStimulation of Wnt signaling directs posterior foregut epithelium into the fundic lineage.