SPDP-SPDP-Crosslinker-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemESPDPCat.No.:HY-100216CASNo.:68181-17-9Synonyms:SPDPCrosslinker分⼦式:C₁₂H₁₂N₂O₄S₂分⼦量:312.36作⽤靶点:ADCLinker作⽤通路:Antibody-drugConjugate/ADCRelated储存⽅式:-20°C,protectfromlight,storedundernitrogen*该产品在溶液状态不稳定，建议您现⽤现配，即刻使⽤。溶解性数据体外实验DMSO:155mg/mL(496.22mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.2014mL16.0072mL32.0143mL5mM0.6403mL3.2014mL6.4029mL10mM0.3201mL1.6007mL3.2014mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现⽤现配，即刻使⽤.体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(6.66mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(6.66mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性SPDP(SPDPCrosslinker)⼀种短链交联剂，可通过NHS-酯和吡啶⼆硫醇反应性团与半胱氨酸巯形成可降解(cleavable)的(可还原的)⼆硫键，实现胺与巯的结合。SPDP(SPDPCrosslinker)⼀种可降解(cleavable)的ADClinker，可⽤于合成抗体偶联药物(ADC)[1]。IC50&TargetDisulfideCleavableCleavable体外研究Equalamountsofanti-CD11candanti-CTLA-4Abs(inboratebufferedsaline;pH8.5)areactivatedusingSPDP.户使⽤本产品发表的科研⽂献•BioengTranslMed.26July2022.•MicrobCellFact.2021Mar10;20(1):67.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LobedanzSetal.Aperiplasmiccoiled-coilinterfaceunderlyingTolCrecruitmentandtheassemblyofbacterialdrugeffluxpumps.ProcNatlAcadSciUSA,2007Mar13,104(11):4612-7.[2].Karumuthil-MelethilSetal.Dendriticcell-directedCTLA-4engagementduringpancreaticbetacellantigenpresentationdelaystype1diabetes.JImmunol2010Jun15,184(12):6695-708.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-