CTX-IELISAKitI型胶原C末端肽ELSIA试剂盒说明书

第第页CTX—IELISAKitI型胶原C末端肽ELSIA试剂盒说明书TypeICollagenCtelopeptide,CTXI,AssayKitCatalog#6033INTRODUCTIONCollagenisthemostabundantproteininthemammalianbodyandlendsstructuralintegritytotissuesastheprimarycomponentoftheextracellularmatrix（1）.TypeIcollagenisthemaincomponentofbone,tendon,skin,andothertissues（2）.Infact,typeIcollagenmakesup20%ofbonesbymass,whichaccountsformorethan90%oftheorganiccomponents.Asaresult,degradationproductsoftypeIcollagencanbedetectedinserumandurineinstagesofbonelossormetabolismandcanbepotentialmarkersofbonemetabolism（3）.ProteinasesmediateresorptionoftypeIcollagenfromboneandgeneratespecificpeptidefragmentsofdegradedcollagen.Forexample,matrixmetalloproteinases（MMPs）exclusivelyproduceCterminaldegradedfragments（ICTP）fromtypeIcollagen,whilecathepsinKproducesIfragmentsfromtheCterminusandNTXIfragmentsfromtheNterminusoftypeIcollagen（4）.BecausetheproteinaseactivitiesdifferamongdiseasesanddegradedfragmentsreflectthemetabolismoftypeIcollagen,immunoassayshavebeendevelopedtomonitorthelevelsofthesedegradedfragmentsinbiologicalfluids（5）.Patientswithosteoporosisdisplayreducedbonemass.Duringdiseaseprogression,degradedpeptidesoftypeIcollagenareobservedinserumaswellasurine.Therefore,ICTPandNTXIhavebeenusedasmarkersofosteoporosis（68）.Inaddition,itwasreportedthatCTXIlevelsinurinearecorrelatedwithdiseaseactivityofosteoarthritis（9）.Furthermore,cancerswhichmetastasizetobonecanaffectthemetabolismoftypeIcollagen.SerumCTXIlevelscorrelatewithprognosisofthesecancers,especiallyprostate,lung,breast,andurinarybladdercancersThus,degradedtypeIcollagenfragmentsareveryusefultoolsforevaluatingdiseasenotonlyinhumans,butalsoinmice,leadingtothedevelopmentofmanymousediseasemodelsforcancer,osteoporosis,osteoarthritis,andrheumatoidarthritis.Chondrex,Inc.hasdevelopedaCTXIdetectionELISAkitformouseandhumanusingacomp