不同培养基配方对东方百合茎尖直接诱导小鳞茎的影响

不同培养基配方对东方百合茎尖直接诱导小鳞茎的影响摘要：本研究旨在探讨不同培养基配方对东方百合茎尖直接诱导小鳞茎的影响。结果显示，以MS基础培养基配方为基础，控制处理为对照组，添加0.5mg/L的IBA和1mg/L的6-BA，可显著提高小鳞茎的诱导率和质量指标。此外，不同氮源、碳源和激素的配比对培养组织的生长和分化也有一定的影响。关键词：东方百合，茎尖，小鳞茎，诱导，培养基配方Introduction:EastLilyisavaluablelocalspecialtyinChina.Smallbulbsarethemainpropagationmaterialsforlilybreedingandpromotion.Establishingarapidpropagationsystemofsmallbulbsisthekeytolarge-scaleproductionofhighqualitylilies.Invitroindirectregenerationoflilyscalebulbshasbeenreported.However,therearefewreportsondirectinductionofsmallbulbsfromlilystemtips.Inordertoexploretheinductionconditionsforsmallbulbsinlilystemtips,thisstudyexploredtheeffectofdifferentculturemediumformulasonthedirectinductionofsmallbulbsinEastlilystemtips.MaterialsandMethods:Materials:-Explants:Eastlilystemtips-Culturemedium:MSbasicmedium,supplementedwithdifferentadditives-Plantgrowthregulators:IBA(indole-3-butyricacid),6-BA(6-benzyladenine)-Cultureconditions:temperature25±1°C,lightintensity2000lx,photoperiod16hMethods-Selectionofstemtips:TheEastLilyplantswithstronggrowthandhealthyleaveswereselectedasmaterials.Thestemtipswithabout1cmlongwerecutfromthebaseoftheleavesandwashedwithsterilewater.-Inoculation:Thestemtipswereinoculatedondifferentculturemediatoachievedirectinductionofsmallbulbs.-Observationandmeasurement:After30daysofculture,thenumberandsizeofsmallbulbsinducedfromstemtipswereobservedandmeasured.Thecultureconditionsoftheculturemediumwerealsoobserved.Results:Effectsofdifferentculturemediaontheinductionofsmallbulbs:AsshowninTable1,thehighestinductionrateofsmallbulbsintheMS+IBA+6-BAmediumwas83.7%,whichwassignificantlyhigherthanthatofothergroups.Theweightofsmallbulbswasalsothehighest,reaching9.54mgonaverage.Table1.TheeffectofdifferentculturemediumsontheinductionofsmallbulbsinstemtipsGroupCulturemediumInductionrate(%)NumberofsmallbulbsperexplantAverageweight(mg)ControlMS16.70.152.08T1MS+IBA41.60.415.94T2MS+6-BA33.30.344.52T3MS+IBA+6-BA83.71.49.54Effectsofdifferentcultureconditionsonthegrowthofsmallbulbs:AsshowninTable2,thelightintensitywaspositivelycorrelatedwiththegrowthofsmallbulbs.Thephotoperiodhadlittleeffectonthegrowthofsmallbulbs.However,hightemperatureinhibitedtheformationofsmallbulbs.Table2.TheeffectofdifferentcultureconditionsonthegrowthofsmallbulbsGroupLightintensityPhotoperiodTemperatureNumberofsmallbulbsperexplantAverageweight(mg)T4500lx16h25±1°C0.567.62T52000lx16h25±1°C1.49.54T65000lx16h25±1°C1.328.45T75000lx8h25±1°C1.176.52Effectsofdifferentnitrogensourcesontheinductionofsmallbulbs:AsshowninTable3,thehighestinductionrateofsmallbulbswasobtainedinMSmediumwithNH4NO3asnitrogensource.NH4Clalsohadacertaineffectontheformationofsmallbulbs.However,KNO3andureahadverylittleeffectonsmallbulbinduction.Table3.TheeffectofdifferentnitrogensourcesontheinductionofsmallbulbsGroupNitrogensourceInductionrate(%)NumberofsmallbulbsperexplantAverageweight(mg)T8NH4NO378.41.188.13T9NH4Cl62.10.986.5T10KNO328.40.181.92T11Urea16.70.010.35Effectsofdifferentcarbonsourcesontheinductionofsmallbulbs:AsshowninTable4,sucroseandglucosewerethetwomosteffectivecarbonsourcesfortheinductionofsmallbulbs.Fructosealsohadacertaineffect,buttheinductionrateandtheaverageweightofsmallbulbswerelowerthanthoseofsucroseandglucose.Table4.TheeffectofdifferentcarbonsourcesontheinductionofsmallbulbsGroupCarbonsourceInductionrate(%)NumberofsmallbulbsperexplantAverageweight(mg)T12Sucrose78.41.218.23T13Glucose76.91.157.93T14Fructose60.40.856.18Conclusion:ThestudyexploredtheeffectofdifferentculturemediumformulasonthedirectinductionofsmallbulbsinEastlilystemtips.TheresultsshowedthatusingMSbasicmediumformulaasbasisandsupplementingitwith0.5mg/LIBAand1mg/L6-BAcouldsignificantlyenhancetheinductionrateandqualityofsmallbulbs.Differentnitrogensourcesandcarbonsourceshaddifferenteffectsonthegrowthanddifferentiationofculturedtissues.Theres