全自动血细胞分析显微镜复检规则解读及复检方法-文档在线预览

全自动血细胞分析显微镜复检规则及复检方法解读复检规则制定的原则:、复检规则制定的依据及验证结果四、复检后报告方式三、复检规则及复检方法复检规则制定的原则-1.根据该仪器对细胞形态的识别能力。-2.复检规则涉及的参数该仪器均能提供。-3.复检范围应涵盖仪器的所有参数及形态学特征。•4.在确保病理细胞不漏检、保证筛选质量的基础上尽量降低复检率，使患者能在较短的时间内获得正确的检验报告。•5.假阴性是制定复检规则的关键参数，具有诊断意义的重要参数不能出现假阴性，其他参数的假阴性率应＜5%。•6.在较低假阴性率的前提下降低假阳性率，假阳性率过高会增加工作量，延迟报告发放时间。•7.白血病患者及临床医生提出阅片需求的样本必须镜检。复检规则制定的依据及验证结果•1.根据2005年国际血液学复检专家组推荐的41条自动血细胞分析和分类复检规则。•2.根据本实验室的具体情况及SysmexXE-2100的性能特点，参考国际41条复检规则，制定了适合本实验室的17条显微镜复检规则。3.验证结果：真阳性率10.6%假阳性率16.8%真阴性率69.6%假阴性率3%复检率27.4%复检规则及复检方法4-7X109/L;7-9X109/L;10-12X109/L13-18X109/LHGBRBC:RBC直方图出现双峰或多峰WBC:WBC彡30123涂片镜检彡200g/L或＜60g/L，涂片镜检。,涂片镜检。0X109/L或＜2.5X109/L的初诊患者WBC数量的估计方法2-4/HP4-6/HP6-10/HP10-12/HP复检规则及复检方法图异趴滿明『尾部抬高觀值的縱’涂片矗方检，如结果不符，应人工计数。-PLT数量的估计方法：-如PLT数为80X109/L，RBC数为4.00X1012/L,油镜观察5-10个视野每400个RBC可见8个左右的PLT。提示PLT聚集，必需镜检。即使未提示PLT聚集，但PLT较低，特别注意观察片尾。explorerfiascopendingUpperLowervalidatecp&nmenuNamecommentWBC「ItemsWEC3PLT聚集手工计数175h~i二opyPLTAbnDStR.esearch(w)|Research(R^i||curnu1ative|q-f1ags|service|hpc■WBCQ-ifferentialRETRBCPLTZ5OFLWBC/BASOb<DIFFItemDataUnitMEUT%81.2+%LYMPH%14,0-%MONO%3.9%EQ%0.6%BAS。尨0.3%ItemDataUnitWBCRBCHGBHCTMCVMCHMCHCPLT6.204.6013641.991.129.632575ifir10A9/L10A12/Lg/L%fLpgg/L10A9/LRDW-SDRDW-CVPDWMPVP-LCRPCT46.013.9fL%fL什%%RET%RET#IRiFLFRMFRHFR%10A12/L%%%%NRBC#NREC%10A9/L/100WECSampleNo.f8251Birth厂Ward[Date12009/05/12Sexpat.id

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