限制性内切酶保护碱基表

PCR设计弓I物时酶切位点的保护碱基表酶寡核苷酸序列切割率％2hr20hrGGTCGACC00AccICGGTCGACCG00CCGGTCGACCGG00CACATGTG00AflIIICCACATGTGG>90>90CCCACATGTGGG>90>90GGCGCGCC>90>90AscIAGGCGCGCCT>90>90TTGGCGCGCCAA>90>90CCCCGGGG50>90AvaICCCCCGGGGG>90>90TCCCCCGGGGGA>90>90CGGATCCG1025BamHICGGGATCCCG>90>90CGCGGATCCGCG>90>90CAGATCTG00BglIIGAAGATCTTC75>90GGAAGATCTTCC25>90GGCGCGCC00BssHIIAGGCGCGCCT00TTGGCGCGCCAA50>90BstEIIGGGT(A/T)ACCC010AACTGCAGAACCAATGCATTGG00BstXIAAAACTGCAGCCAATGCATTGGAA2550CTGCAGAACCAATGCATTGGATGCAT25>90CATCGATG00ClaIGATCGATC00CCATCGATGG>90>90CCCATCGATGGG5050

EcoRIGGAATTCCCGGAATTCCGCCGGAATTCCGG>90>90>90>90>90>90GGGGCCCC>90>90HaeIIIAGCGGCCGCT>90>90TTGCGGCCGCAA>90>90CAAGCTTG00HindIIICCAAGCTTGG00CCCAAGCTTGGG1075GGGTACCC00KpnIGGGGTACCCC>90>90CGGGGTACCCCG>90>90MluIGACGCGTC002550CGACGCGTCGNcoICCCATGGG005075CATGCCATGGCATGCCATATGG00CCCATATGGG00NdeICGCCATATGGCG00GGGTTTCATATGAAACCC00GGAATTCCATATGGAATTCC75>90GGGAATTCCATATGGAATTCCC75>90GGCTAGCC00NheICGGCTAGCCG1025CTAGCTAGCTAG1050TTGCGGCCGCAA00ATTTGCGGCCGCTTTA1010NotIAAATATGCGGCCGCTATAAA1010ATAAGAATGCGGCCGCTAAACTAT2590AAGGAAAAAAGCGGCCGCAAAAGGAAAA25>90NsiITGCATGCATGCA10>90>90>90CCAATGCATTGGTTCTGCAGTTTTAATTAA00PacIGTTAATTAAC025CCTTAATTAAGG0>90

GTTTAAAC00PmeIGGTTTAAACC025GGGTTTAAACCC050AGCTTTGTTTAAACGGCGCGCCGG75>90GCTGCAGC00TGCACTGCAGTGCA1010PstIAACTGCAGAACCAATGCATTGG>90>90AAAACTGCAGCCAATGCATTGGAA>90>90CTGCAGAACCAATGCATTGGATGCAT00CCGATCGG00PvuIATCGATCGAT1025TCGCGATCGCGA0101010SacICGAGCTCGSacIIGCCGCGGC0500>90TCCCCGCGGGGAGTCGACGTCAAAAGGCCATAGCGGCCG00C1050SalIGCGTCGACGTCTTGGCCATAGCGGCCG1075CGGACGCGTCGACGTCGGCCATAGCGGCCGCGGAASeaIGAGTACTC10752575AAAAGTACTTTTCCCGGG010SmaICCCCGGGG010CCCCCGGGGG1050TCCCCCGGGGGA>90>90GACTAGTC10>90SpeIGGACTAGTCC10>90CGGACTAGTCCG050CTAGACTAGTCTAG050GGCATGCC00SphICATGCATGCATG025ACATGCATGCATGT1050

AAGGCCTT>90>90StuIGAAGGCCTTC>90>90AAAAGGCCTTTT>90>90CTCTAGAG00XbaIGCTCTAGAGC>90>90TGCTCTAGAGCA75>90CTAGTCTAGACTAG75>90CCTCGAGG00XhoICCCTCGAGGG1025CCGCTCGAGCGG1075CCCCGGGG00XmaICCCCCGGGGG2575CCCCCCGGGGGG50>90TCCCCCCGGGGGGA>90>90Apal(类型:TypeIIrestrictonenzyme)识别序列：5GGGCC93'BamHI(类型:TypeIIrestrictionenzyme)识别序列:5'GAGATCCBglll(类型：TypeIIrestrictionenzyme)识别序列：5'AAGATCT3'EcoRI(类型:TypeIIrestrictionenzyme)识别序列：5'GAAATTC3'HindHI(类型:TypeIIrestrictionenzyme)识别序列：5'AAAGCTT3'Kpnl(类型：TypeIIrestrictionenzyme)识别序列:5'GGTACAC3'NcoI(类型：TypeIIrestrictionenzyme)识别序列：5'CACATGG3'NdeI(类型:TypeIIrestrictionenzyme)识别序列：5'CAATATG3'NheI(类型:TypeIIrestrictionenzyme)识别序列：5'GACTAGC3'NotI(类型:TypeIIrestrictionenzyme)识别序列：5'GCAGGCCGCSacI(类型:TypeIIrestrictionenzyme)识别序列：5'GAGCTAC3'Sall(类型:TypeIIrestrictionenzyme)识别序列：5'GATCGAC3'SphI(类型：TypeIIrestrictionenzyme)