C12FDG-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEC12FDG(solution)Cat.No.:HY-DY1033CASNo.:138777-25-0分⼦式:C₄₄H₅₅NO₁₆分⼦量:853.92作⽤靶点:FluorescentDye作⽤通路:Others储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性C12FDG(5-Dodecanoylaminofluoresceindi-β-D-Galactopyranoside)(solution)⽤于β-半乳糖苷酶检测的亲脂性绿⾊荧光底物。C12-FDG⽐Fluoresceindi(β-D-galactopyranoside)(HY-101895)对动物细胞β-半乳糖苷酶的活性测定更敏感(Ex/Em=488/523nm)。溶剂及浓度：DMSO：10mM体外研究Guidelines(Followingisourrecommendedprotocol.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds).1.Solutionpreparation[2]1.1StoragesolutionStorage:Afteraliquoting,storeat-20°Cor-80°Cinthedarkandavoidrepeatedfreezingandthawing.1.2PreparationofworkingsolutionDilutewithPBS(orotherbuffer).Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:Theworkingsolutionshouldbepreparedandusedimmediately,andprotectedfromlight.2.Fluorescentsenescence-associatedβ-galactosidase(SA-β-Gal)assay:2.1Culturecellsin6-,12-,24-,or96-wellplatesatadensityof5×105cells/mLovernight.Incubatethecellsaccordingtoyournormalprotocol.2.2Washcellsby200μLofPBSonceandfixwith100μLoffixationsolution(2%formaldehyde/0.2%1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEglutaraldehydeindistilledwater)atroomtemperaturefor5min.2.3Washcellsby200μLofPBStwotimes,andstainwith100μLof33μMC12FDG(inPBS,pH=6.0)for10min,andwith200μLofHoechstsolution(1μg/mLHoechst33342(HY-15559)inPBS,pH6.0)for10min.2.4Imagethesecellsbya20×objectiveand360-nm(Hoechst33342)and480-nm(C12FDG)excitationfilters,andmonitorthrough460-nmand535-nmemissionfilters,respectively.REFERENCES[1].PlovinsA,etal.Useoffluorescein-di-beta-D-galactopyranoside(FDG)andC12-FDGassubstratesforbeta-galactosidasedetectionbyflowcytometryinanimal,bacterial,andyeastcells.ApplEnvironMicrobiol.1994Dec;60(12):4638-41.[2].UdonoM,etal.Quantitativeanalysisofcellularsenescencephenotypesusinganimagingcytometer.Methods.2012Mar;56(3):383-8.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Fo