雨课堂学堂在线学堂云《细胞生物学实验（南方科技）》单元测试考核答案

第1题WhichofthefollowingisNottheessentialequipmentforcellculture?ACellculturehoodBCentrifugeCSpectrophotometerDIncubator第2题WhyisCO2oftenusedinanincubatortoculturemammaliancells?AtocontrolthepHBtocontrolorpreventviral/bacterialcontaminationCtocontrolcellgrowthDtocontroloxygenconsumption第3题ThecellcultureenvironmentincludesAThegascompositionBMediaandmediacomponentsCTheplasticthecellsareattachedtoDAlltheabove第4题第5题Whichoftheannulardiaphragmsshouldbeusedunder4xobjectivelens(PhL)whenusingphase-contrastmicroscope?APhLannulardiaphragmBPh1/PhCannulardiaphragmCPh2annulardiaphragm第6题Whichofthefollowingmicroscopytechniquesreliesonthespecimeninterferingwiththewavelengthoflighttoproduceahighcontrastimagewithouttheneedfordyesoranydamagetothesample?AConventionalbrightfieldlightmicroscopyBPhasecontrastmicroscopyCElectronmicroscopyDFluorescencemicroscopy第7题第8题第9题Whatprocessdoesthefollowingdiagramrepresent?APrimaryCellCultureBCloningCCellpropagationDCellpreservation第10题Afterthefirstsubculture,theprimarycultureiscalledACloneBdaughtercellsCcelldebrisDcellLine第11题____________referstotheremovalofcellsfromamulticellularorganismalongwithfewgrowthfactorsandgrowninafavorableenvironment.ACellCultureBCellDivisionCCellIsolationDCellproliferation第12题WhichofthefollowinginNOTthepartofgrowthmediumforanimalculture?ASerumBInorganicsaltsCStarchDCarbonsource第13题Namethetypeofculturewhichispreparedbyinoculatingdirectlyfromthetissueofanorganismtoculturemedia?ASecondarycellcultureBPrimarycellcultureCCelllinesDCellstrains第14题Whatisacellline?ATransformedcellsBMultilayercultureCMultiplegrowthofthecellsDSubculturingofprimaryculture第15题Plottingasemi-logarithmicgraphoftherateofcellproliferationovertimeAgrowthcurveBlogphaseCcontactinhibition第16题Inthesecondaryculture,cellsareobtainedfromAprimarycultureBorgancultureCphenotypiccultureDtheorganism第17题第18题Whichofthefollowingcelllinesisepithelial-likemorphology?ACHO-K1BNIH/3T3CU-937第19题Whichofthefollowingcelllinesisfibroblast-likemorphology?ACHO-K1BNIH/3T3CU-937第20题Whichofthefollowingcelllinesislymphoblast-likemorphology?ACHO-K1BNIH/3T3CU-937第21题Whatisthepurposeofaddingtrypsintocellcultureintheexperiment?ADivisionofcellsBCultureofcellsCStainingofcellSDDeaggregationofcells第22题Whichofthefollowingisthepurposeofusingcentrifugeforcellculture:AToseparateproteinBTospindowncellsCToextractDNAfromcellsDToremoveculturemedium第23题Thetotalnumberofcellsinacultureiscountedusingthetrypanblueexclusionassayandisfoundtobe6.8x106cells/ml.Eachwellina6wellplaterequires2x105cells.Howshouldthesolutionbedilutedsothat1mlcanbeaddedtoeachwell?A1:6.8then1:10B1:10then1:2C1:3.4then1:10D1.3.4then1:2第24题Thetotalnumberofcellsinacultureiscountedusingthetrypanblueexclusionassayandisfoundtobe2.3x106cells/ml.Thecultureisdiluted1:23andthen100μlseededperwellintoa96wellplate.Whatisthefinalcelldensityperwell?A1x105B2.7x104C2.7x105D1x104第25题第26题第27题Whatarethetwomajortypesofcellculturecontaminants?I.ChemicalII.OrganicIII.BiologicalIV.MacroscopicAIandIIIBIIandIIICIandIVDIIIandIV第28题第29题TypesofcontaminationthatyoucannotseewithastandardinvertedmicroscopeincludethefollowingAFungalBBacterialCMycoplasma第30题Oh,no!Oneofyourdishesofmammaliancellshasbacterialcontamination!Whatisthemostlikelyreason?AYouforgottoaddpenicillinandstreptomycintoyourmediaBYouexposedthecellstotrypsinfortoolongCYoutouchedthedishDYoufedthecellstoooften第1题Whatisthedifferencebetweentransienttransfectionandstabletransfection?ATransienttransfectionintroducesforeigngeneticmaterialforashortperiodoftime,whilestabletransfectionintegratesthematerialintothehostcell'sgenome.BTransienttransfectionusesphysicalmethods,whilestabletransfectionuseschemicalmethods.CTransienttransfectiononlyproducessmallamountsofprotein,whilestabletransfectionproduceslargeramounts.DTransienttransfectionisonlyusedinresearch,whilestabletransfectionisusedinindustryandmedicalapplications.第2题Whatisthepurposeofusingaselectablemarkerintransfection?AToidentifycellsthathavetakenupforeigngeneticmaterialBTopreventthegrowthofuntransfectedcellsCTotrackthemovementofforeigngeneticmaterialwithinthecellDAlloftheabove第3题WhichofthefollowingisNOTacommonchemicaltransfectionreagent?ALipofectamineBPolyethylenimineCAntibioticsDCalciumphosphate第4题Whichtypeoftransfectionisthemostefficientintermsofforeigngeneticmaterialuptake?AChemicaltransfectionBPhysicaltransfectionCViraltransfectionDThereisnoclearwinneramongthedifferenttypesoftransfection第5题ADNAvaccineis:AADNAmoleculethatisrecognizedbyanantibody.BAvaccinethatworksbystimulatingtheimmunesystemtorecognizepathogenDNAsequences.CAvaccinethatisadministeredasDNA;theDNAisthenexpressedtoproduceaprotein,whichstimulatesanimmuneresponse.第6题Whattypeofmoleculesarecommonlyusedtodeliverforeigngeneticmaterialintoacellduringtransfection?APlasmidsBRNACProteinsDLiposomes第7题Genetransfertoanimalcells.Thedifferencebetweentransfectionandtransductionis:AIntransfection,thetransgeneisinsertedinaplasmid,whileintransduction,thetransgeneisinsertedinaviralgenome.BTransfectioninvolvesthetransferofnakedDNAintothecellwhiletransductioninvolvespackagingtheDNAintoavirusparticle,whichtheninfectsthecell.CThereisnodifference-thetermsaresynonymous.第8题Whatistheprocessofintroducingforeigngeneticmaterialintoacellcalled?ATransductionBTranscriptionCTranslationDTransfection第9题第10题WhatisGFP?AAdyeusedtostainDNAforfluorescencemicroscopy.BAfluorescenceproteinusedtotagproteinsofinterestincells.CAstructurefeatureofprokaryoticcells.DAtypeofpowerfulelectronmicroscope.第11题Whatwillaffecttheefficiencyoftransfection?ATypeofcellsBGenerationofcellsCPurityofplasmidDNADConfluencyofcellsEMethodoftransfectionFAlloftheabove正确答案：F第12题第13题第14题第15题Whichsubstanceiscommonlyusedasafluorescentdyeinbiomedicalresearch?AMercuryBSodiumchlorideCFluoresceinDCalciumcarbonate第16题Whichnaturalphenomenonisanexampleoffluorescence?AThebluecoloroftheskyBTherainbowCBioluminescenceinfirefliesDRedcolorofripestrawberries第17题Influorescence,theemittedlighthas:AThesamewavelengthastheabsorbedlightBAlongerwavelengththantheabsorbedlightCAshorterwavelengththantheabsorbedlightDNorelationshipwiththeabsorbedlight'swavelength第18题WhichofthefollowingisNOTrequiredforasubstancetoexhibitfluorescence?AHightemperatureBAbsorptionoflightCEmissionoflightDElectronictransitions第19题Whatisfluorescence?ATheemissionoflightoflowerenergyafterabsorbinghigherenergylightBTheemissionoflightoflowerenergyafterabsorbinghigherenergylightCTheemissionoflightofhigherenergyafterabsorbinglowerenergylightDTheemissionoflightwiththesameenergyastheabsorbedlight第三章章节测试第1题第2题第3题WhichofthefollowingwouldresultinNOchangeinosmoticpressureacrossamembrane?AThesolutescandiffusethroughtheporesandtheconcentrationofsolutesisthesameonbothsidesofthemembrane.BTheconcentrationofsolutesisthesameonbothsidesofthemembrane.CWaterismovingwithitsconcentrationgradient.DThesolutescandiffusethroughthepores.正确答案：AB第4题第5题Whichofthefollowingsolutionscontainstheleastsolute?AHypertonicBIsotonicCHypotonic第6题Whichofthefollowingisnotrequiredforosmosistooccur?AcellularenergyBselectivelypermeablemembraneCWaterDconcentrationgradient第7题Whichofthefollowingisleastlikelytoincreasetherateofdiffusion?AhigherconcentrationofmoleculesBsmallmoleculesizeChightemperatureDsmallconcentrationgradient第8题第9题Whichofthefollowingismostlikelytomovethroughthecellmembranebyfacilitateddiffusion?ACO2BO2CsmalllipidsDNa+第10题Whichofthefollowingisnotapassiveprocess?AfacilitateddiffusionofglucoseBNa+/K+pumpCoxygendiffusionDosmosis第11题Certainhormonesbindtoreceptorsattheplasmamembraneto“deliver”theirmessagetothecell.Whatfunctionoftheplasmamembraneisthis?APhysicalbarrierBCommunicationCSelectivelypermeableDCell-to-cellrecognition第12题Whichofthefollowingisnotamajorfunctionofproteinsinthecellmembrane?AformingchannelsBanchoringcellstootherstructuresCformingtheentireglycocalyxDactingasreceptors第13题第14题第15题Thebloodcellsmembraneactsasatruesemipermeablebarrierforasalt.Thusanisoosmoticsolutionofthissaltisalsotermedisotonic:ATrueBFalse第16题VitaminCsolutionwithaconcentrationof5.05%(w/v)isiso-osmoticwithblood.However,thissolutioncauseshemolysis.Thus,thissolutionisalsoisotonic:ATrueBFalse第17题第四章章节测试第1题Howmanychromosomesareshowninthispicture?Howmanychromatids？A4chromosomes,4chromatidsB4chromosomes,8chromatidsC8chromosomes,4chromatidsD4chromosomes,8chromatids第2题第3题第4题Whichofthefollowingcouldnotbethesourceforhumankaryotypeanalysis?ALymphocytesBBonemarrowCTumorcellsDMatureredbloodcells第5题第6题第7题第8题第9题Whenyoudoakaryotypeanalysis,thecellsshouldbearrestinwhichphase?AmetaphaseBProphaseCanaphaseDtelophase第五章章节测试第1题Whichcellsareconsideredimmortal?ABloodcellsBCardiacmusclecellsCStemcellsDNeurons第2题Whichisasourceofadultstemcells?ABonemarrowBAmnioticfluidCEmbryosDGametes第3题Whatisthedevelopmentalpathwaybywhichthehematopoieticstemcellgivesrisetoamacrophage?Athehematopoieticstemcelldivides,givingrisetoadaughtercellthatremainsastemcellandadaughtercellthatdifferentiatesdirectlyintoamacrophage.Bthehematopoieticstemcellcangiverisetoeitheralymphoidprogenitororamyeloidprogenitor;themyeloidprogenitordifferentiatesdirectlyintoamacrophage.CThehematopoieticstemcellcanfollowtwopaths,onecalledlymphoidandtheothercalledmyeloid,eitherofwhichcanultimatelygiverisetomacrophagesandothercellDThehematopoieticstemcellgivesrisetoavarietyofcellsreferredtoasgranulocytes,anyoneofwhichcandifferentiatefurtherintoamacrophage.Ethehematopoieticstemcellmustfirstgenerateacellofthemyeloidlineage(asopposedtothelymphoidlineage),thenagranulocyte/macrophageprogenitor,thenamonocyte,andfinallyamacrophage.正确答案：E第4题Whichofthefollowingcellswouldbeconsidereddifferentiated?ABlastomereBMusclecellCStemcell第5题第6题第7题Whenyoudoakaryotypeanalysis,thecellsshouldbearrestinwhichphase?AmetaphaseBProphaseCanaphaseDtelophase第六章章节测试第1题第2题第3题第4题第5题Whichistheblanksample(cellproliferationisinhibited)inthisexperiment?AHU(hydroxyurea)-,EdU(5-ethynyl-2’-deoxyuridine)-BHU-,EdU+CHU+,EdU+第6题Whichisthecontrolsample(cellproliferationisinhibited)inthisexperiment?AHU(hydroxyurea)-,EdU(5-ethynyl-2’-deoxyuridine)-BHU-,EdU+CHU+,EdU+第七章章节测试第1题Cyclin-dependentkinase(Cdk)andcyclinworktogethertoregulatethecellcycle.ThediagrambelowshowsthelevelsofCdkandcyclinthroughoutthecellcycle.WhichstatementbestexplainshowCdkandcyclincontrolthecellcycle?ACdkandcyclinaresynthesizedduringtheSandG2phasesanddegradeattheendoftheMphase,becausebothproteinsneedtobepresentinhighlevelsforthecelltoentertheMphaseandbothmustdegradeforthecelltoentertheG1phase.BAnallostericinhibitorbindstoCdkattheendoftheMphaseandallowsthecelltoentertheG1phase,becausewithouttheinhibitionofCdk,thecellwouldremainintheMphaseandbeunabletogrow.CCyclinissynthesizedduringtheSandG2phasesandisdegradedattheendoftheMphase,sothecyclin-CdkcomplexcannotcontinuetoregulatemitoticprocessesandthecellenterstheG1phase.DAslevelsofcyclinincreasethroughouttheMphase,cyclinservesasanegativefeedbackinhibitorbyblockingthefunctionofCdksothattheMphaseceasesandtheG1stagebegins.第2题第3题第4题Whichofthefollowinggroupsofproteinsassociatewithkinasesandaresynthesizedanddegradedatspecificpointsduringthecellcycle?ACyclinsBGrowthfactorsCCyclindependentkinasesDSurvivalfactors第5题HowdoesasomaticcellthathasjustcompletedtheSphaseofitscellcyclecompareinrespecttoitsnumberofchromosomesandamountofDNAwithagameteofthesamespecies?AIthastwicethenumberofchromosomesandtwicetheamountofDNABIthasthesamenumberofchromosomesbuttwicetheamountofDNACIthastwicethenumberofchromosomesandfourtimestheamountofDNA.DIthasfourtimesthenumberofchromosomesandtwicetheamountofDNA第6题第7题Whenacyclin-dependentkinase(Cdk)bindstoacyclinprotein,thetwoformaproteincomplexthatregulatesaportionofthecellcycle.ManyorganismshavemultipletypesofCdksandcyclinsthatworktogethertoguideacell’sprogressthroughthephasesofthecellcycle.Onecyclin-Cdkcomplexiscalledmaturation-promotingfactor(MPF).MPFallowsthecelltoadvancefromtheG2stageofthecellcycletotheMphasebyactivatingmanyotherproteins.Anothercyclin-Cdkcomplex,referredtoasS-Cdk,activatesproteinsduringtheSphaseofinterphase.WhichpredictionwouldbethemostlikelyresponsetohighlevelsofS-CdkcomplexandlowlevelsofMPFinacell?AAnenzymethatdegradesthenuclearenvelopewouldbeactivated,becausethisdegradationoccursearlyintheMphase.BAnenzymethatinitiatesDNAreplicationwouldbeactivated,becauseDNAmustbereplicatedbeforecelldivisionoccursintheMphase.CAnenzymethatinitiatesthetightcoilingofchromatinintothickthreadswouldbeactivated,becausethiscoilingoccursbeforechromosomescanbedivided.DAnenzymethatbeginsspindleassemblywouldbeactivated,becausespindleassemblyisimportantinthesegregationofsisterchromatidsduringanaphase.第8题Inflowcytometry,whatisforward-scatterameasureof?AGranularityBFluorescenceintensityCSizeDDensity第9题Inflowcytometry,whatisside-scatterameasureof?AGranularityBFluorescenceintensityCSizeDDensity第10题DNAreplicationduring_____ofthecellcycle.AG1BSCMDG2第11题Whichofthefollowingmutatedproteinsismostlikelytocauseacelltobecomecancerous?AAnoveractiveversionofproteinthatstimulatescelldivisionBAnunderactiveversionofaproteinthatstimulatesgrowthhormonereceptorsCAnoveractiveversionofaproteinthatpromoteapoptosisDAnunderactiveversionofaproteinthatpromotescellcycleprogression第八章章节测试第1题第2题Monocytesmovefromthesystemiccirculatorysystemintogeneralconnectivetissues,wheretheydifferentiateintowhatphagocyticcelltype?AMacrophageBTcellsCBcellsDNeutrophil第3题Whichtypeofimmunecellisthefirsttoarriveatthesiteofaninjurytostartcleaningup?AMacrophageBMonocyteCNeutrophilDdendriticcell第4题Whichofthefollowingarereasonsthatacellmightengageinphagocytosis?ATofeedBTodestroyaninvaderCTocleanupcellulardebrisDAllanswersarecorrect第九章章节测试第1题1.

Whatdoyouhavetoadjusttoensurethatfluorescencefromonechanneldoesn’tspilloverintotheotherchannel?ACompensationBVoltageCGatesDThreshold第2题第3题Inaflowcytometeryouwillseethecellslackingboththelabelsin____________quadrant.A1stB2ndC3rdD4th第4题第5题Polyclonalgoatanti-mouseIgGantibodyconjugatedtohorseradishperoxidaseis_____.ATheprimaryantibody.BTheantigenthatprimaryantibodybindsto.CThesecondaryantibody.DTheantigenthat