CN119751638A 一种猴降钙素原重组蛋白、单克隆抗体及其制备方法和应用

(12)发明专利申请(10)申请公布号CN119751638A(21)申请号202510269257.5司(72)发明人胡勤芹杨飞张念元王振平权利要求书1页说明书9页序列表(电子公布)附图1页(54)发明名称23检测项目明显升高不升高或略升高明显升高快，幅度更大略升高明显升高明显升高45[0021]实施例1gagcggcagcctgcatgcggcgccgtttcgcgatggccgcgaaagcagcccggatcctgctgctggcggcgctggtgcaggattatgtgcagatgaacaaatttgaactgggcagcagcctggatagcaaacgcagcaaacgctgcggcaacctggatgcgtgcattaacaaatttgcgacctttccgcagaccgcgattggcgtgggcgcgccgatgagcagcgatcgccgctatgtgagcatgc进行初步粗纯化，再利用离子交换层析得到纯化后的重组蛋白即为猴降钙素原重组蛋白，67编号猴降钙素原1234567898actgagctgcgtggcgagcggcacccgcggcggctttaccagcatgacctgggtgcgctggaatggattgcgagcatttatggcagcggcaaccgcacctattatccggatattagccgcgatgatgcgaaaagcattctgtatctgcagatgaacagcctgcgcagcgaagattgcgtgcgcggcagctattatttttatagcaacttttttgatacctatggccagggcgtggaccctgaactgcaaagcgagccagaactatcgcggcaacctggcgtggtatcagcaaaatttctgatttttaacagcaaaagcctggaaaccggcgtgccgagccgctttaattttaccctgaccattagcagcctgcagccggaagatgtggcgacctatttttcacctttggcggcggcaccaaac[0046]SEQIDNo.8:caggtgaccctgaaagaaagcggcctgacctgcagctttagcggcaccctggatcgcacctatttttatggcgtgagctaaaggcctggaatggctggcgcatattacctttaacggcaccaaacgctataac9ccattagcaaagataccagcagcaaccaggtgtttctgaaaattaccagcgtggttattgcgcgcgcggcagctttgatcgcaccagctattttctggtgtggggcgcgggcaccacgaccattagctgcaaagcgagccagaccaacagctatacccgctatagctatagggccagccgccgaaactgctgatttatgcggcgagcaacctggaaagcggcattccggcagcggcaccgattttaccctgaacattcatccggtggaagaagaagatgcggcgcggcggcagcccgtatacctttggcggcggcaccaaactgaaatttagcccgtttctggcgctgagcattctggtgctgctgcaggcgggcagccggattatgtgcagatgaaagcgagcgaactggaacaggaacaggaaaccgaaggcagagcaaacgctgcggcaacctgagcacctgcatgctgggcacctatacccaggacgcagaccgcgattggcgtgggcgcgccgggcaaaaaacgcgatatgagcagcgat[0051]表2灵敏度测试数据标曲浓度冲液冲液PBS缓冲液192345/16//71/08//[0053]表3灵敏度测试数据标曲浓度192345//6//7//08//