Frozen-Section-Embedding-Medium-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEFrozenSectionEmbeddingMediumCat.No.:HY-DY3004作⽤靶点:BiochemicalAssayReagents作⽤通路:Others储存⽅式:Roomtemperaturefor1year,protectfromlight.Donotfreeze*Insolvent:-80°C,6months;-20°C,1month(Room

temperaturefor1year,protectfromlight.Donotfreeze)BIOLOGICALACTIVITY⽣物活性FrozenSectionEmbeddingMedium(OCT)⼀种⽔溶性聚⼄⼆醇和聚⼄烯醇混合物，⼴泛⽤于免疫组织化学实验。FrozenSectionEmbeddingMedium在冰冻切⽚过程中⽀撑组织，增加组织连续性，减少皱缩和碎⽚化。体外研究Guidelines(Thefollowingareourrecommendedprotocols.Thisprotocolisforreferenceonlyandshouldbemodifiedaccordingtoyourspecificneeds.)Method1:1.Removethetissueblockfromthesucrosesolutionandimmerseitina1:1volumeratiomixtureof20g/LsucroseandFrozenSectionEmbeddingMediumembeddingagent.Soakatroomtemperaturefor2hours.2.TransfertheabovetissueintoFrozenSectionEmbeddingMediumembeddingmediumandsoakatroomtemperaturefor4hours;thenreplacewithfreshFrozenSectionEmbeddingMediumembeddingmediumandcontinuesoakingatroomtemperaturefor6hours.3.Placethespecimenonthestageandsectionitusingacryostat,toathicknessof10μm.Mountthesectionsontopretreatedglassslidesandstoreat-20°C.MethodTwo:1.Setthecryostatoperatingtemperaturetotherangeof-10°Cto-25°C.2.Applyalayerofembeddingmediumtothesurfaceofthesampleholder/disc,thengentlypressthetissueintothedesignatedpositiononthesampleholder/disc,andapplyappropriateembeddingmediumaroundthetissue.Note:1.Tissueorembeddingblocksmaydetachfromthesupportduetoinsufficientembeddingmediumor1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEmisalignmentofthetissuewithintheembeddingmedium.2.Sectiontearingmaybeduetooverfreezingofthetissueordamageoruncleanlinessofthecuttingedge.3.Sectionthawingmaybeduetoincorrecttemperatureofthecoldchamberandthecuttingedge.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:4