LT-104A-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemELT-104ACat.No.:HY-179407CASNo.:2574572-03-3分⼦式:C₃₃H₃₄O₇分⼦量:542.62作⽤靶点:Phosphodiesterase(PDE)作⽤通路:MetabolicEnzyme/Protease储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性LT-104A⼀种有效的PDE4抑制剂，可提⾼细胞内cAMP⽔平（EC50=1.9μM），并抑制PDE4D3活性(IC50=9.3μM)。LT-104A能激活cAMP-PKA-CREB抗炎通路并抑制NF-κB相关因表达(Il1bandNos2)，可⽤于炎症相关疾病研究[1]。IC50&TargetPDE4D39.3μM(IC50)体外研究LT-104A(1-30μM;24h)reducesLPS(HY-D1056)-inducedIl1bandNos2mRNAexpressioninJ774A.1macrophages[1].LT-104A(1-30μM;24h)decreasesLPS-inducednitricoxideproductioninJ774A.1macrophages[1].LT-104A(0.1-30μM;30min)increasesintracellularcAMPlevelsinHEK293cellsinaconcentration-dependentmanner(EC50=1.9μM)[1].LT-104A(0.1-30μM;6h)enhancesCRE-luciferaseactivitythroughactivationofthecAMP-PKA-CREBsignalingpathwayinHEK293cells[1].LT-104A(0.1-100μM)inhibitsrecombinantPDE4D3enzymaticactivitywithanIC50of9.3μM[1].RealTimeqPCR[1]CellLine:LPS(1μg/mL,24h)inducedJ774A.1macrophagesConcentration:1,3,10,30μMIncubationTime:30minbeforeLPStreatmentfor24h.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEResult:ReducedLPS-inducedIl1bandNos2mRNAexpression.REFERENCES[1].CoffeyC,etal.IdentificationofanovelPDE4inhibitorinspiredbyleoligin-derivedlignans.EurJMedChem.2026;302(Pt1):118285.doi:10.1016/j.ejmech.2025.118285McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;021-58955