Methoxy-X04-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEMethoxy-X04(solution)Cat.No.:HY-DY1045CASNo.:863918-78-9分⼦式:C₂₃H₂₀O₃分⼦量:344.41作⽤靶点:Amyloid-β作⽤通路:NeuronalSignaling储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性Methoxy-X04(solution)⼀种荧光染料，可以穿过⾎脑屏障，选择性地结合在致密中⼼淀粉样蛋⽩Aβ斑块中的β-折叠层。Methoxy-X04保留对淀粉样蛋⽩Aβ(AAβ)原纤维的体外结合亲和⼒(Ki=26.8nM)。Methoxy-X04具有荧光性，可对阿兹海默症(AD)⼤脑死后切⽚中的斑块、缠结和脑⾎管淀粉样蛋⽩进⾏染⾊，具有良好的特异性[1][2]。溶剂及浓度：DMSO：5mg/mL体内研究Methoxy-X04(5-10mg/kg,i.p.,oncetime)differentiatesindividualplaquesinPS1/APPmicewithin30to60min[2].1.Methoxy-X04SolutionInjection24hrpriortoperfusion,weighthemiceandgiveeachmouseanintraperitonealinjectionofmethoxy-X04atadoseof10mg/kgofbodyweight.2.TranscardialPerfusionoftheInjectedMice3.BrainSectioningUsingaVibratome4.SectionScreeningforthePresenceofMethoxy-X04-stainedPlaquesExamineeachsectionsuccessively,toavoiddryingoutthesections,usingthefollowingprocedure:AddadropletofPBStoamicroscopeslidewithadisposablepipette,andplacethesectiononthedroplet.Examinethesectionunderafluorescentmicroscope(excitation340-380nm)toidentifyregionscontainingMethoxy-X04labeledAβplaques.REFERENCES1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].KlunkWE,etal.ImagingAbetaplaquesinlivingtransgenicmicewithmultiphotonmicroscopyandmethoxy-X04,asystemicallyadministeredCongoredderivative.JNeuropatholExpNeurol.2002Sep;61(9):797-805.[2].BishtK,etal.CorrelativeLightandElectronMicroscopytoStudyMicroglialInteractionswithβ-AmyloidPlaques.JVisExp.2016Jun1;(112):54060.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;