细环病毒-从实验室到临床

1、.,细环病毒：从实验室到临床,Torquetenovirus: the human virome from bench to bedside,文献汇报,D. Focosi et al. Clinical Microbiology and Infection 22 (2016) 589-593,.,TTV: Transfusion transmitted virus or Torquetenovirus From torques and tenuis, Latin for necklace and thin, respectively 输血后肝炎（肝中病毒载量是血中的10-100倍，并且与血清A

2、LT升高相关）【现已否定该结论】,Human virome,at least 29 major species,细环病毒：首个被发现的单链环状DNA病毒,.,TTV prevalence 95% in most cases independent of age, socioeconomic standing and health conditions 来源：胎盘、呼吸道、输血 分布：T淋巴细胞 现有临床应用： TTV as a Marker of Anthropic Pollution TTV as a Marker of Immune Status,Orphan virus孤儿病毒（非病原性

3、病毒）,.,检测水源是否被污染： 现有方法为检测细菌，但是很容易出现假阳性 某些情况下即使细菌检测为阴性也不能排除被污染 检测病毒更准确 TTV分布广泛：医院空气、物件表面均可存在,TTV as a Marker of Anthropic Pollution,TTV as a Marker of Immune Status,免疫功能完整性： the ability to mount a protective immune response against an antigenic stimulation. 现有检测：抗原特异性，抗原非特异性；检测繁琐，检测方法缺乏标准化 TTV与CD8+57+

4、T淋巴细胞比例成负相关 对抗病毒药不敏感,.,TTV as a Marker of Immune Status,excessive (too many opportunistic infections and cancers),inadequate (too many episodes of cellular acute rejection),Maintenance immunosuppression,.,Why Is TTV Not Yet Part of a Diagnostic Tool Kit?,现有研究不足 无法培养 无抗体检测试剂 无动物模型 PCR方法缺乏标准化（引物设计） 特异性引物 非特异性引物,.,Take home messages TTV is a type of Orphan virus, prevalence95% in most cases TTV replicates mainly in T lymphocytes Insensitive to current antiviral drugs TTV as a Marker of Immu