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Improving the quality and safety of frozen muscle foods by emerging freezing technologies: A review,intruduction,Freezing is one of the most wide spread used preservation methods for meats including sh meat. Traditional freezing methods such as air blast freezing and cryogenic freezing could induce some quality deterioration such as damage to cell structure, increased drip loss, and poor sensory value. Therefore, novel freezing methods have been developed to minimize the disadvantages of traditional freezing methods.,High pressure freezing,01,Electrically and magnetically assisted freezing,02,Ultrasound-assisted freezing,03,Antifreeze proteins,04,CONTENT,conclusion,05,1,High pressure freezing,PART ONE,1.High pressure freezing,High pressure freezing (HPF) utilizes different states of water under pressure. As shown in Fig. 1 under high pressures and different temperatures, water usually form multiple kinds of ice (I-VI) by different phases. Therefore, HPF can be classied into three types: high pressure induced freezing (HPIF, ABEFHI in Fig. 1), high-pressure shift freezing (HPSF, ABEFG in Fig. 1) and high-pressureassisted freezing (HPAF, ABCD in Fig. 1) with HPSF being the most common option.,HPF can not only improve freezing process and the quality of frozen food, but can also have positive effects on food safety due to microbial inactivation,Figure 1. Phase diagram of water (ABCD for pressure assisted freezing, ABEFG for pressure shift freezing, and ABEFHI for pressure induced freezing) (Cheng et al.,2015).,2,Electrically and magnetically assisted freezing,PART TWO,Electrically assisted freezingMagnetically assisted freezingElectromagnetic assisted freezing,2.Electrically and magnetically assisted freezing,the orientating or rotating movements of polar molecules become stronger under the impact of the electric eld,Electrically assisted freezing,a magnetic eld can change the freezing characteristics of water molecules,Magnetically assisted freezing,induce the dipole rotation, which cause disturbance in ice nucleation, formation and growth,Electromagnetic assisted freezing,In recent years, efforts have been made to apply electromagnetic assisted during freezing for controlling the formation and growth of ice crystals and thus three technologies have emerged: electric eld, magnetic eld, and electro-magnetic eld assisted freezing methods.,1,2,3,1.Electrically assisted freezing,From the thermodynamic point of view, when relative change in the Gibbs free energy for the system became negative, the nucleation process takes place spontaneously.,orlowska (2009)studied the change of Gibbs free energy during liquid crystallization under an applied electrically assisted freezing (EF), and showed that the free energy of a system under freezing decreases due to the polarization of molecules in the system affected by the strength of the applied eld.,Therefore ice nucleation process occurs when the Gibbs free energy of the system is minimized, thus explaining the reason of applying an electric eld to lead to the early formation of ice crystals and the enhancement of the nucleation process in the system,Therefore, freezing under electric eld or electric disturbance, both OEF and SEF show positive effects on the quality of frozen model foods,2.Magnetically assisted freezing,The mechanism of the inuence of magnetically assisted freezing (MF) on food samples is closely associated with the magnetism of water molecules, and the linkage between water molecules in the samples would become closer as more numbers of hydrogen bonds are formed, which makes the network become more stable (Cai et al., 2009), inducing an increase of the thermal conductivity and freezing point of water (Inaba et al., 2004). Therefore, MF seems to have the ability to control the supercooling and the subsequent growth of ice crystals.,A commercial unit for applying magnetic elds in freezing biological tissues was patented and developed a Japanese company (ABI Corporation Ltd, Chiba, Japan), in which induction coils and permanent magnets are used to develop a weak oscillating magnetic eld, leading to delay of the formation of ice crystals in supercooled water.,3.Electromagnetic assisted freezing,Similar to EF and MF, microwave assisted freezing is based on inherent characteristics of water, in which microwaves can induce the dipole rotation, which cause disturbance in ice nucleation, formation and growth.,As discussed above, electrical and magnetic impacts could interact with water molecules, rearrange the hydrogen bonds and make the network of water stronger.,However, in order to achieve the same effects, more powerful magnetic elds are needed, since it was proved that electric elds were more efcient in altering the conformation of water network compared with the magnetic eld, which meant that the impact of electromagnetic wave on water molecules was mostly resulted from the electrical disturbances,Chaplin (2013) suggested that, besides microwaves at 915 and 2450 MHz, radiofrequency radiation (RF) at 27.12 MHz or even at extremely low frequency of 3300 Hz can affect water molecules signicantly and lastingly.,3.Electromagnetic assisted freezing,01,02,03,Microwave is not the only kind of electromagnetic wave that can exert force on water molecules,Therefore, it seems that electromagnetic waves in several frequency bands should be able to assist the freezing process in varying degrees.,3,Ultrasound-assisted freezing,PART THREE,3.Ultrasound-assisted freezing,Ultrasound assisted freezing is mainly based on the cavitation and micro-streaming effects generated by the ultrasound. It has the advantages of intensifying heat transfer, promoting the formation of ice crystal and fracturing large ice crystals. The cavitation effect would produce and even made cavities or bubbles collapse violently, with microbubbles acting as nuclei for formation of ice crystals and the collapse of cavitation bubbles enhancing the mass and heat transfer. Although studies show that, as a potential tool, ultrasound can be used to make ice crystals in model foods smaller, applications of ultrasound assisted freezing are still limited, in particular few investigations are focused on meat freezing.,4,Antifreeze proteins,PART THREE,4.Antifreeze proteins,In the tissues and blood of the shes inhabiting polar and northern coastal waters, a family of proteins called antifreeze proteins (AFPs) existed to prevent shes from freezing, which can decrease the freezing point and have thermal hysteresis activity It is generally accepted that AFPs can interfere with propagation of water molecule to surface of ice crystal, limiting the spaces for ice lattice to grow and thus reducing the stability of the surface at the ice water interface . Therefore, AFPs can modify and even control the growth and aggregation of ice crystals, during temperature uctuations to inhibiting nucleation formation and altering growth rate and habit of ice crystals,5,conclusion,PART five,This r
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