【精品】度冷丁对肠道平滑肌运动机能的影响99

度冷丁对肠道平滑肌运动机能的影响二系二班 6组度冷丁l度冷丁学名哌替啶，又称作唛啶、地美露。其盐酸盐为白色、无嗅、结晶状的粉末，能溶于水，一般制成针剂的形式。作为人工合成的麻醉药物，度冷丁普遍地使用于临床，它对人体的作用和机理与吗啡相似，但镇痛、麻醉作用较小，仅相当于吗啡的 1 10-1 8，作用时间维持 24 小时左右。毒副作用也相应较小，恶心、呕吐、便秘等症状均较轻微，对呼吸系统的抑制作用较弱，一般不会出现呼吸困难及过量使用等问题。目的l研究其对动物离体和在体肠道平滑肌运动机能的影响，并初步探讨其作用机理材料l药品：度冷丁（ pethidine）纳洛酮（ naloxone） ，由浙江大学医学院提供l动物：豚鼠 1只 ;l 小鼠六只，体重 20.1 1.4g以上动物雌雄兼用，由浙江大学医学院提供仪器l张力换能器l微机生物信号采集仪l麦氏浴槽方法l 1.1离体肠道平滑肌实验 豚鼠禁食 24h，致死后迅速制备回肠平滑肌标本，置 32摄氏度恒温 Kreb氏液中，持续通以 95氧气和 5二氧化碳混合气体，负荷 1g（肠管），通过张力换能器，记录平滑肌（纵行肌）收缩张力。稳定 20分钟后加杜冷丁 0.2ml,记录收缩曲线，换液，稳定 10min；加杜冷丁 0.2ml,当收缩达最大时加纳洛酮 0.2ml，待收缩曲线平稳后加入同等量杜冷丁，记录收缩曲线。方法l1.2肠推进运动实验小鼠随机分组，禁食 18h后，按 0.2ml/10g容量 iv(尾静脉）给药，杜冷丁、杜冷丁 +纳洛酮；对照组等容量生理盐水。给药后10min，按 0.4ml/10g ig酚红糊剂（含酚红0.1mg/ml）。此后，分别于 10， 30， 60min处死动物，取出全段小肠，度量小肠中酚红糊前沿至幽门的长度，计算下指标：小肠酚红推进率酚红糊推进距离（ cm） /幽门至回盲部长度（ cm） 100表 1 度冷丁对豚鼠小肠平滑肌张力的影响组 最大收 缩张 力（ g） 度冷丁 度冷丁 +纳 洛 酮123456_x s表 2 度冷丁对小鼠小肠酚红移动率的影响组别 剂 量 酚 红 移 动 率 （ ml/10g） 10min 20min 30min杜冷丁 组杜冷丁 +纳 洛 酮组生理 盐 水 组原理l哌替丁 Pethidine （度冷丁）为人工合成镇痛药，与吗啡相似，作用于阿片受体而发挥作用。能中度提高胃肠道平滑肌及括约肌张力，减少推进性蠕动，但因作用时间短，故不引起便秘，也无止泻作用。能引起胆道括约肌痉挛，提高胆道内压力，但比吗啡弱 。Thank you 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