光源在园艺与药用植物和人工虫草室内培养中的应用ppt课件

LED光源在园艺与药用植物和 人工虫草室内培养中的应用 华南师范大学生命科学学学院 李海航,博士、教授、博导 2014年6月21日，广州 广东省植物生长调节剂协会与广州市果树科学研究所 2014年植物生长调节剂与现代农业研讨会 植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光 不同的生物及不同生长发育期需要不同的光质与光强 降温 光在植物与食用菌等的室内培养中广泛应用 光照 研究内容 非洲菊组织培养 不定芽诱导、芽增殖和生根 铁皮石斛和金钗石斛组培繁殖 原球茎增殖、生长、分化 虫草子实体形成,生长,功能成分 子实体的诱导形成 生长：高、鲜重、干重 生物活性物质: 虫草素,多糖,类胡萝卜素 Explant: Dedifferentiation callus Redifferentiation Single shootCluster shoots Rooting Plantlets Multiplication Division Adventitious buds LED treatment Shoot multiplication procedure Compared to WFL, no significant differences were observed in the treatments of V1,V2, V5 and V6. V3 and V4 decreased the FW of the shoots. V6 increased dry weight significantly. Fresh weight and dry weight Height and number of shoots All LEDs could increase the height of shoots except V5. But No.of shoots/explant had no significant different. Chlorophyll content All LEDs induced chlorophyll biosynthesis significantly, compared with WFL W V1V2V3V4V5V6WFL 35 days cultured in vitro under LED V1 V4 V3 V6 V2 V5 WFL 35 days cultured in vitro under LED Explant: Dedifferentiation callus Redifferentiation Rooting Plantlets Results Root induction Receptacle Multiplication Division Adventitious buds Cluster shoots Root induction under LED Single shoot V3 treatment decreased FW and DW. V2 treatment increased FW and DW significantly. Fresh weight and Dry weight Heights of shoots under V1, V2, V3 and V4 were significantly higher than control. V2 had the most number of roots compared with others. Shoot height and root number V3, V4 and V5 promoted the root activity significantly. V2 increased the maximum root length. Root activity and maximum root length V1V2V3V4 V5V6WFL Seedlings 35 d after rooting induction Survival rateHeight (cm)No. of leaves V1 V2 V3 V4 V5 V6 WFL 100% 100% 100% 100% 100% 100% 100% 9.460.22 a 9.770.22 a 8.190.34 b 9.540.30 a 8.020.27 b 8.250.48 b 7.090.23 c 5.190.23 b 4.940.11 b 6.060.19 a 6.000.35 a 5.310.24 b 6.190.20 a 4.600.27 b Results Transplantation 15 days after transplantation, no difference on seedlings survival rates. All LED treated seedlings were significant higher and number of leaves increased. V4 V1 V5 V2 V6 V3 WFL Transplanted seedlings grown for 15 days Among 6 LEDs, V1,V2 and V6 were suitable for shoot multiplication and V2 was good for root induction. Survival rates of seedlings from all LED treatments had no difference after seedling transplantation. But the seedlings grown under LEDs were higher with more leaves than WFL. Conclusions Jie Ye 叶婕Hongmei Bao 包红梅QingshengYe 叶庆生 II - Dendrobium officinale组培繁殖 Tissue culture process shoots Plantlets(1-2cm) seedling (7-8cm) germination 1 month growth and differentiation 2 month seeds growth 1 month protocorms shoot elongation 3 month Plantlets 27LED treatment FW of plantlet(g) bc abc c c After 90 days growth, FW of plantlet under V6 was the highest, but there were no significant differences compared to control. 30 days60 days90 days 0.1 0.0 0.2 0.3 V1 V3 V2 V4 V6V5 FL a d d aaba a b abab a ab a c cbcab FW of plantlets V6 DW of plantlet(g) DW of plantlets under V6 was significantly higher than others after 90 days treatment. 30 days60 days90 days 0.00 0.01 0.02 0.03 ab ab ab b a aa a a a a V1V2 V3V4 V6V5 FLab a c abcaba a ab DW of plantlets c V6 Height of plantlet(cm) There were some different in plantlet height after 30 and 60 days irradiation under LED, the different disappeared after 90 days. 30days60days90days 0.5 0.0 1.5 2.0 V1V2 V4 V6 V3 V5 FL a a a a bab a a c bc a a a a a bcbc a bcbc Height of plantlets a 90天后差异不显著 Stem diameter (cm) bcbc cd Similar results were observed in stem diameter 30days60days90days 0.0 0.2 0.3 V2 V4 V6 V1 V3 V5 FL d cd a ab ab b d ab a a a a ab ab abab a b Stem diameter V6 Tillering Compared with other treatments, number of tillering under V6 was significantly higher than FL. 60days90days 0 1 2 a a V1V2 V3V4 V6V5 FL a a a a b bc a bc bc d c a Tillering 分蘖 V6 V6 Roots of plantlet After 60 days cultivation, there were more roots under V6. 60days90days 2 0 4 V1 V3 V2 V4 V5V6 FL a aa a a cd a ab b d ab c a Roots number per plantlets a Growth of plants after 30 days under LED. Growth of plants after 60 days cultivation under LED. Growth of plants after 90 days cultivation under LED. Among 6 LED, V6 were suitable for plantlet growth, it promoted the DW, diameter, tillering and rooting in Dendrobium officinale. Conclusions III - Cordyceps militaris ZhiliYi 易智莉Haihang Li 李海航 本实验室培养 的蛹虫草 选出对蛹虫草的生长、产品质量最优的光质， 为LED光源在蛹虫草栽培中的运用提供理论和应用基础。 不同的光照时间蛹虫草色素的变化 初步探究虫草色素的光诱导形成过程与机理。 重 复 3 次 原基诱导形成 材料与方法 材料与方法 重 复 3 次 子实体生长实验 材料与方法 甲醇浸提 光照组组合光质质红红/蓝蓝/远红远红 1远红远红 光/ 2红红光/ 3红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 424 4红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 325 5红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 334 6红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 145 7红红光+蓝蓝光+远红远红 光红红蓝蓝远红远红 = 163 8蓝蓝光/ 9白光红红蓝蓝远红远红 = 631 10荧荧光灯（对对照）红红蓝蓝远红远红 = 460 LED光照处理 结果与讨论 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 1. 黑暗、红红光、远红远红 光下不能形成 子实实体原基 2. 蓝蓝光和含蓝蓝光的LED组组合都能 诱导诱导 原基形成。表明蓝蓝光在子实实 体原基诱导诱导 形成中起决定性作用 3. LED白光（R/B=2/1）和 LED处处 理（R/B=2/1）对对原基的诱导诱导 形 成较较好 1.光质对子实体原基形成的影响 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 处处理鲜鲜重最高 其他LED处处理组组与荧荧光对对照组组 无显显著差异 远红远红 光下鲜鲜重最低 结果与讨论 2.光质对子实体生长（鲜重）的影响 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 （R/B/Fr=4/2/4）干重较较高 远红远红 光下干重最低,其次是红红 光 -处处理都含蓝蓝光，干重无 显显著差异，但都高于远红远红 光和红红 光 结果与讨论 3.光质对子实体生长（干重）的影响 结果与讨论 各处处理的子实实体平均高度无统统 计计学差异 处处理的高度略高于其它 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 4.光质对子实体生长（平均高度）的影响 I. FR (100%) II. R (100%) VII. R:B:FR 1:6:3 VIII. B (100%) III. R:B:FR 4:2:4 IV. R:B:FR 3:2:5 V. R:B:FR 3:3:4 VI. R:B:FR 1:4:5 IX. LED white X. Fluorescent LED白光虫草素含量最高，比对对 照高62%； 其次是LED红红光，比对对照高60% 其他LED组组合比荧荧光对对照都有不 同程度的提高。 红红光有利于提高虫草素的含量， 蓝蓝光对对虫草素积积累的作用不显显著。 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 结果与讨论 5.光质对虫草素含量的影响 62% 60% 各光照处处理下虫草多糖的含 量无统计统计 学差异。 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 结果与讨论 6.光质对虫草多糖含量的影响 R Fr B W FL R/B/Fr=4/2/4 R/B/Fr=3/2/5 R/B/Fr=3/3/4 R/B/Fr=1/4/5 R/B/Fr=1/6/3 LED蓝蓝光下类类胡萝萝卜素含量最高 ，比对对照组组提高了51.6%。 LED远红远红 光下类类胡萝萝卜素含量最 低，比荧荧光对对照低54.3%。 其次是红红光，比对对照低46.4%。 蓝蓝光和含蓝蓝光的LED处处理，类类胡 萝萝卜素都比对对照高，且蓝蓝光比例 越高，类类胡萝萝卜素含量越高。 结果与讨论 7.光质对类胡萝卜素含量的影响 R 光诱导色素合成 光诱导子实体原基形成 （形态建成） 色素的形成