穴位肥大细胞及其颗粒物在针刺镇痛中的作用PPT课件.ppt

穴位肥大细胞及其颗粒物在针刺镇痛中的作用,2010.10.16,Outline,穴位肥大细胞在针刺镇痛效应中的作用穴位胶原纤维在针刺镇痛效应中的作用穴位注射组胺的镇痛效应手针和电针穴位信号的启动机制,Mastcellsinacupointsandsideward,THEJOURNALOFSCIENCEP0.01,P0.05vs.GroupB1;P0.01,P0.05vs.GroupD1,1.Therelationshipbetweenmastcellfunctionandacupunctureeffect,穴区组织肥大细胞脱颗粒率统计(n=6),Note:*P0.01,*P0.05vs.ControlGroup;P0.01vs.AcupunctureGroup.,ResultsfromExperiment,StainingofMastCellsinAcupointonRats,Muscle,Skin,BeforeAcupuncture,AfterAcupunctureDegranulationofcells,AfterSDCGStabilized,THEJOURNALOFSCIENCE&HEALING,2008，4（3），170-177,Whatisthekeyfactortodegranulation,Theacupunctureeffectispositiverelationshiptothedegranulationofmastcellatacupoint.Whatiskeyfactorconducethedegranulationofmastcellduringacupuncturemanipulation?Ourfocusisonthecollagenatacupoint.,2019/12/10,13,2.collagenfibersintheacupointduringmanualacupuncture,ToobservethefunctionofcollagenfibersinMAanalgesia.7Animalgroupssetting:,normalgroup(N):Normalhealthyanimalcontrolgroup(M):AnimalwithAAinjectionofcollagenaseatZusanli(ST36)group(C)manualacupuncturegroupwithrotationmanipulation(R)orlift-thrustmanipulation(L),rotationgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CR),lift-thrustgroupwithcollagenasepretreatmenttodestroyZusanli(ST36)collagenfibers(CL),lift-thrustgrouppretreatedwithcollagenasetodestroyDubi(ST35)collagenfibers(CDL),(R),(L),2019/12/10,14,2.collagenfibersintheacupointduringmanualacupuncture,Analgesiaeffectsobservedindifferentgroups,注：较正常组痛阈：*P0.01；较处理前痛阈：P0.05；较捻转组(R)痛阈变化率：P0.05；较捻转组(L)痛阈变化率：P0.05；较正常组或模型组:P0.05；较捻转组或提插组:P0.05。,2019/12/10,15,2.collagenfibersintheacupointduringmanualacupuncture,Analgesiaeffectsobservedindifferentgroups,注：较正常组痛阈：*P0.01；较处理前痛阈：P0.05；较捻转组(R)痛阈变化率：P0.05；较捻转组(L)痛阈变化率：P0.05；较正常组或模型组:P0.05；较捻转组或提插组:P0.05。,2019/12/10,16,2.collagenfibersintheacupointduringmanualacupuncture,Analgesiaeffectsobservedindifferentgroups,注：较正常组痛阈：*P0.01；较处理前痛阈：P0.05；较捻转组(R)痛阈变化率：P0.05；较捻转组(L)痛阈变化率：P0.05；较正常组或模型组:P0.05；较捻转组或提插组:P0.05。,collagenfibersofskinandmuscle,Mastcellsinskin,GroupNormal,GroupInjectControl,collagenfibersofskinandmuscle,Mastcellsinskin,2.collagenfibersintheacupointduringmanualacupuncture,ConnectiveTissueResearch,2008，50（2）:110-120,Acupunc.whirling,Acupunc.liftandthrust,collagenfibersofskinandmuscle,Mastcellsinskin,collagenfibersofskinandmuscle,Mastcellsinskin,2.collagenfibersintheacupointduringmanualacupuncture,ConnectiveTissueResearch,2008，50（2）:110-120,Mastcellsdegranulationbeforeandaftercollagendestroyed,2.collagenfibersintheacupointduringmanualacupuncture,Collagendestroyed,Collagendestroyed,ConnectiveTissueResearch,2008，50（2）:110-120,collagenfibersandmastcellsinnormalacupoint,collagenfibersenlacementandmastcellsdegranulationwhilewhirling,collagenfiberstightenedandmastcellsdegranulatedwhileliftingandthrusting,collagenfibersweredestroyedandmastcellshadnodegranulation,2.collagenfibersintheacupointduringmanualacupuncture,ConnectiveTissueResearch,2008，50（2）:110-120,2019/12/10,21,2.collagenfibersintheacupointduringmanualacupuncture,Themastcellsinacupointmaytranslatethemechanicalsignalintothebiologicalinformationofneuralsystem.,Thecollagenandmastcellsdegranulationareveryimportanttothemanualacupunctureanalgesiaeffect.,Isthisthesameaselectricacupuncture?,2019/12/10,22,2.collagenfibersintheacupointduringelectroacupuncture,手针及电针治疗前后AA大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化,注：与正常组相比较：*P0.05；与模型组相比较：P0.05；与手针组相比较：P0.05；P0.01；与电针组相比较：P0.05。,3.穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,3.穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,针刺研究，2010，35（2）：99-103,3.穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,针刺研究，2010，35（2）：99-103,3.组胺H1受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,P0.01与空白组比较（vs.control）；P0.01与模型组比较（vs.model）；P0.01与手针针刺组比较（vs.control）；P0.01与生理盐水穴位注射组比较；P0.01与组胺穴位注射组比较，P0.01与组胺H1拮抗剂穴位注射组比较。,针刺研究，2010，35（2）：99-103,3.组胺H1受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,P0.01与空白组比较（vs.control）；P0.01与模型组比较（v