生化双语试卷1(西北).pdf

西西 北北 农农 林林 科科 技技 大大 学学 2003 2004 学年第一学期生物技术 生物工程专业 生 物 化 学 双语教学 课程期末考试AB卷 A卷卷 命题教师命题教师 赵惠贤 审题教师审题教师 考试班级 考生姓名 考生学号 考生成绩 一一 Term explanations chose five of seven to finish 20 points 4 for each the active site of an enzyme Tm oxidative phosphorylation glycolysis promoter codon an operon 二二 Fill in the blank 10 points 0 5 for each 1 The competitive inhibitor binds reversibly to the active site of an enzyme Thus there is in the Vmax of the enzyme but Km 2 Photosynthesis uses solar energy to synthesize carbohydrate from carbon dioxide and water In the light reactions the light energy drives the synthesis of and In the dark reactions carbon fixation reactions carbohydrate is synthesized 3 Glycolysis is a series of reactions that take place in of all prokaryotes and eukaryotes and it converts one molecule of into giving a net yield ATPs 4 Three amino acids and contribute to most of the ultraviolet UV absorbance of proteins which absorb maximally at 280nm 5 During DNA replication synthesizes multiples primers on the lagging strand and elongates the lagging strands at its 3 end then removes the lagging strand RNA primers and finally joins the lagging strand fragment together 6 Of 64 codons of protein biosynthesis 和 are stop codons is the initation codon 7 The sequence of a DNA fragment is GCTACTAAGC the base sequence of its complementary strand is 1 三三 Mono choice 15 points 1for each 1 The linkage style of the glycosidic bond in pseudouridine molecule is A C C B C N C N N D All above are wrong 2 Allosteric enzymes are A monoenzymes B oligomeric enzymes C multienzyme system D michaelis Menten enzyme 3 Which of the following compounds isn t belong to high energy compound A glucose 6 phosphate B ATP C PEP D succinyl CoA 4 Which of the following amino acids has positive charge at physiological pH A Cys B Glu C Lys D Trp 5 In animal organs the most important intermediate which make fatty acid from glucose is A pyruvate B ATP C acetyl CoA D acetoacetate 6 Which one of the following reductant is used for fatty acid synthesis A NADH B NADPH C FADH2 D Ferredoxin red 7 Which one of the following amino acids can directly convert into ketoglutarate A Asp B Ala C Glu D Gln 8 The oxidative decarboxylation of pyruvate is catalized by A a monomeric enzyme B a oligomeric enzyme C the multi enzyme complex D Michaelis menten enzyme 9 When was the semi conservative mechanism of DNA replication demonstrated experimentally by Meselson and Stahl A in 1944 B in 1953 C in 1958 D in 1966 10 hn RNA refers to A the collection of pre mRNAs B the collection of pre tRNAs C the collection of pre rRNAs D the population of snRNAs 11 Which of the following RNA doesn t required post transcription modification to have biological function A tRNA B rRNA C prokaryotic mRNA D eukaryotic mRNA 12 If the sequence of a sense strand DNA is 5 ACTGTCAG 3 the sequence of RNA corresponding will be A 5 CUGACAGU 3 B 5 UGACAGUC 3 C 5 ACUGUCAG 3 D 5 GACUGUCA 3 13 A known anticodon of a tRNA molecule is 5 CUG3 the codon it recognizes is A GAC B CAG C IAC D CAI 14 Which of the following component can recognize promoter site and correctly initiate transcription A factor B factor C core enzyme D dnaB protein 15 Proteins synthesis is in the direction of A N terminal to C terminal B C terminal to N terminal C 3 to 5 end D 5 to 3 end 2 四四 True or False 15 points 1 5 for each 1 All DNA in eukaryotic cell are located in nucleus 2 Competitive inhibitors don t affect Km of an enzyme to the substrate it acts on 3 Pentose phosphate pathway can yield ATP so it can take the place of citric acid cycle and as a major source of providing energy for organism 4 All enzymes involved in citric acid cycle locate in mitochondrial matrix 5 The Glycosidic bonds link between base and ribose in nucleotide molecules are the style of C O 6 All DNA molecules in eukaryotes are in nucleus 7 Either in E coli or in eukaryotes RNA primers are removed by DNA polymerase I during DNA replication 8 The mRNAs either from prokaryotes or from eukaryotes are polycitronic 9 All energy used for protein synthesis is directly supplied by ATP 10 Every species of organism have a set of their own specific codons 11 In eukaryotes tRNA rRNA and mRNA are synthesized by a common RNA polymerase 12 Glycolysis can always take place when O2 lacks or not 13 In eukaryotes mRNAs need to be processed after transcription 14 A mutate of E coli lacking of DNA polymerase can normally replicate its chromosome DNA 15 The direction of genetic message following can only is from DNA to RNA and not from RNA to DNA 五五 Question and answer chose five of six to answer 30 points 6 for each 1 What are the action forces maintaining three dimensions structures or tertiary structure of proteins 2 What is enzyme inhibition What are competitive and noncompetitive inhibition What s the differences between them 3 What factors do ensure the fidelity of DNA replication 4 What s the citric acid cycle or tricarboxylic acid cycle and its biological sense or it role in organism 5 How was the semi conservative mechanism of DNA replication demonstrated experimentally by Meselson and Stahl 6 What are the techniques used in genetic code deciphering How did they work 3 六六 Calculation 10 points The following data were the record of a reaction catalyzed by