荧光染料激发和发射光谱表.xls

荧光素名称激发波长 nm 发射波长 nm 备注 1 5 IAEDANS336490 1 8 ANS372480 4 Methylumbelliferone385502 5 carboxy 2 7 dichlorofluorescein504529 5 Carboxyfluorescein 5 FAM 492518 5 Carboxynapthofluorescein pH 10 512 598563 668Ratio Dye pH 5 Carboxytetramethylrhodamine 5 TAMRA 542568 5 FAM 5 Carboxyfluorescein 492518 5 HAT Hydroxy Tryptamine 370 415520 540 5 Hydroxy Tryptamine HAT 370 415520 540 5 ROX carboxy X rhodamine 578604 5 56 67 7591 5 TAMRA 5 Carboxytetramethylrhodamine 548552 5 54 42 2568 6 Carboxyrhodamine 6G518543 6 CR 6G518543 6 JOE520548 7 Amino 4 methylcoumarin351430 7 Aminoactinomycin D 7 AAD 546647 7 Hydroxy 4 methylcoumarin360 449 455 9 Amino 6 chloro 2 methoxyacridine412 430471 474 ABQ344445 Acid Fuchsin540630 ACMA 9 Amino 6 chloro 2 methoxyacridine 412 430471 474 Acridine Orange DNA502526 Acridine Orange RNA460650 Acridine Orange both DNA RNA440 480520 650 Acridine Red455 600560 680 Acridine Yellow470550 Acriflavin436520 Acriflavin Feulgen SITSA355 425460 Aequorin Photoprotein 466 Photoprotein AFPs AutoFluorescent Protein Quantum Biotechnologies see sgGFP sgBFP Alexa Fluor 350 346442 3 34 42 2441 Alexa Fluor 430 431540 Alexa Fluor 488 495 492519 520 Alexa Fluor 532 531 532553 554 Alexa Fluor 546 556 557572 573 Alexa Fluor 568 577 578603 Alexa Fluor 594 590 594617 618 Alexa Fluor 633 632650 Alexa Fluor 647 647666 Alexa Fluor 660 668698 Alexa Fluor 680 679702 Alizarin Complexon530 560 580580 624 645 Alizarin Red530 560580 Allophycocyanin APC 630 645655 660 AMC AMCA S345445 AMCA Aminomethylcoumarin 345425 3 34 47 7444 AMCA X353442 Aminoactinomycin D555655 Aminocoumarin 346442 3 35 50 0445 Aminomethylcoumarin AMCA 345425 3 34 47 7444 Anilin Blue600 Anthrocyl stearate360 381446 APC Allophycocyanin 630 645655 660 APC Cy7625 650755 APTRA BTC Ratio Dye Zn2 466 380520 530Ratio Dye Zn2 APTS424505 Astrazon Brilliant Red 4G500585 Astrazon Orange R470540 Astrazon Red 6B520595 Astrazon Yellow 7 GLL450480 Atabrine436490 ATTO TAG CBQCA465560 ATTO TAG FQ486591 Auramine460550 Aurophosphine G450580 Aurophosphine450 490515 BAO 9 Bisaminophenyloxadiazole 365395 BCECF high pH 492 503520 528 BCECF low pH 482520 Berberine Sulphate430550 Beta Lactamase409 447 520 BFP blue shifted GFP Y66H 381 382 383445 447 448 blue shifted GFP Y66H Blue Fluorescent ProteinBlue Fluorescent Protein BFP GFP FRET Bimane398490 Bisbenzamide360461 Bisbenzimide Hoechst 360461 bis BTC Ratio Dye Zn2 455 405529 505Ratio Dye Zn2 Blancophor FFG390470 Blancophor SV370435 BOBO 1462481 BOBO 3570602 Bodipy 492 515490515 Bodipy 493 503533549 Bodipy 500 510509515 Bodipy 505 515502510 Bodipy 530 550528547 Bodipy 542 563543563 Bodipy 558 568558569 Bodipy 564 570564570 Bodipy 576 589579590 Bodipy 581 591584592 Bodipy 630 650 X625642 Bodipy 650 665 X647665 Bodipy 665 676605676 Bodipy Fl504 505511 513 Bodipy FL ATP505514 Bodipy Fl Ceramide505511 Bodipy R6G SE528547 Bodipy TMR542574 Bodipy TMR X conjugate544573 Bodipy TMR X SE544570 Bodipy TR589617 Bodipy TR ATP591620 Bodipy TR X SE588616 BO PRO 1462481 BO PRO 3544570 Brilliant Sulphoflavin FF430520 BTC Ratio Dye Ca2 464 401533 529Ratio Dye Ca2 BTC 5N atio Dye Zn2 459 417517 532Ratio Dye Zn2 Calcein494517 Calcein Blue373440 Calcium Crimson 588 589611 615 Calcium Green501 506531 Calcium Green 1 Ca2 Dye506531 Ca2 Dye Calcium Green 2 Ca2 506 503536 Ca2 Calcium Green 5N Ca2 506532 Ca2 Calcium Green C18 Ca2 509530 Ca2 Calcium Orange549 575 576 Calcofluor White385 395 405437 440 445 Carboxy X rhodamine 5 ROX 576601 Cascade Blue 377420 3 39 98 8423 3 39 99 9 Cascade Yellow 399550 4 40 00 0552 Catecholamine410470 CCF2 GeneBlazer CFDA494520 CFP Cyan Fluorescent Protein430 433 436 453 474 475 476 501 Cyan Fluorescent Protein CFP YFP FRET Chlorophyll480650 Chromomycin A436 460470 Chromomycin A445575 CL NERF Ratio Dye pH 504 514540 Ratio Dye pH CMFDA494520 Coelenterazine Ca2 Dye bioluminescence 429465 Ca2 Dye bioluminescence native molecule Coelenterazine cp Ca2 Dye 430442 Ca2 Dye bioluminescence Coelenterazine f 437473 Ca2 Dye bioluminescence Coelenterazine fcp452 Ca2 Dye bioluminescence Coelenterazine h 437464 Ca2 Dye bioluminescence Coelenterazine hcp 433444 Ca2 Dye bioluminescence Coelenterazine ip441 Ca2 Dye bioluminescence Coelenterazine n 431467 Ca2 Dye bioluminescence Coelenterazine O460575 Coumarin Phalloidin387470 C phycocyanine CPM Methylcoumarin384469 Methylcoumarin CTC400 450602 CTC Formazan Cy2 489506 Cy3 1 8554568 Cy3 5 581598 Cy3 514566 5 55 52 2570 5 55 54 4 Cy5 1 8649666 Cy5 5 675695 Cy5 649 666 670 Cy7 710 743767 805 Cyan GFP433 453 475 501 cyclic AMP Fluorosensor FiCRhR 500517 CyQuant Cell Proliferation Assay480520 Cell Proliferation Assay Dabcyl453 Dansyl340578 Dansyl Amine337517 Dansyl Cadaverine335518 Dansyl Chloride372518 Dansyl DHPE336517 Dansyl fluoride356 none DAPI359461 Dapoxyl403580 Dapoxyl 2374574 Dapoxyl 3373574 DCFDA504529 DCFH Dichlorodihydrofluorescein Diacetate 505535 DDAO463607 DHR Dihydorhodamine 123 505534 Di 4 ANEPPS496705 Di 8 ANEPPS non ratio 488605 4 49 98 8713 DiA 4 Di 16 ASP 456591 Dichlorodihydrofluorescein Diacetate DCFH 505535 DiD Lipophilic Tracer644665 Lipophilic Tracer DiD DiIC18 5 644665 DIDS341415 Dihydorhodamine 123 DHR 505535 DiI DiIC18 3 549 551565 Dinitrophenol349 DiO DiOC18 3 484 487501 502 DiR748780 Lipophilic Tracer DiR DiIC18 7 750779 DM NERF high pH 497 510540 Ratio Dye pH DNP349 Dopamine340 490 520 DsRed558583 Red fluorescent protein DTAF494520 DY 630 NHS621660 Hemicyane label for proteins and DNA DY 635 NHS634664 Hemicyane label for proteins and DNA EBFP383447 Enhanced Blue Fluorescent Protein ECFP436474 Enhanced Cyan Fluorescent Protein EGFP488 498507 516Enhanced Green Fluorescent Protein ELF 97345530 Eosin524545 Erythrosin529 532554 555 Erythrosin ITC529555 Ethidium Bromide510 523595 605 Ethidium homodimer 1 EthD 1 528617 Euchrysin430540 EukoLight Europium III chloride EYFP513 520527 532Enhanced Yellow Fluorescent Protein Fast Blue360440 FDA494520 Feulgen Pararosaniline 570625 FIF Formaldehyd Induced Fluorescence 405433 FITC490 494520 525 FITC Antibody493517 Flazo Orange375 530612 Fluo 3480 506 506520 527 Fluo 4494516 Fluorescein FITC 490 494520 525 Fluorescein Diacetate494520 Fluoro Emerald495524 Fluoro Gold Hydroxystilbamidine 361536 Fluor Ruby555582 FluorX494520 FM 1 43 479598 FM 4 46515640 Fura Red high pH 572657 Fura Red Fluo 3 Fura 2 high calcium335505 Excitation ratio dye Fura 2 low calcium363512 Excitation ratio dye Fura 2 BCECF Genacryl Brilliant Red B520590 Genacryl Brilliant Yellow 10GF430485 Genacryl Pink 3G470583 Genacryl Yellow 5GF430475 GeneBlazer CCF2 GFP S65T 498516 GFP red shifted rsGFP 498516 GFP wild type non UV excitation wtGFP 475509 GFP wild type UV excitation wtGFP 395509 GFPuv385508 Gloxalic Acid405460 Granular Blue355425 Haematoporphyrin530 560580 Hoechst 33258345487 Hoechst 33342347483 Hoechst 34580392440 HPTS355465 Hydroxycoumarin325 360386 455 Hydroxystilbamidine FluoroGold 361536 Hydroxytryptamine400530 Indo 1 high calcium330401 Emission ratio dye Indo 1 low calcium346475 Emission ratio dye Indodicarbocyanine DiD 644665 Indotricarbocyanine DiR 748780 Intrawhite Cf360430 JC 1514529 JO JO 1530545 JO PRO 1532544 LaserPro795812 Laurodan355460 LDS 751 DNA 543712 LDS 751 RNA 590607 Leucophor PAF370430 Leucophor SF380465 Leucophor WS395465 Lissamine Rhodamine572 577591 592 Lissamine Rhodamine B577592 LIVE DEAD Kit Animal Cells494517 for more details refer Calcein Ethidium homodimer528617 to LOLO 1566580 LO PRO 1568581 Lucifer Yellow425 428528 536 540 Lyso Tracker Blue373422 Lyso Tracker Blue White466536 Lyso Tracker Green504 534511 551 Lyso Tracker Red490516 Lyso Tracker Yellow551576 LysoSensor Blue374424 LysoSensor Green442505 LysoSensor Yellow Blue384540 Mag Green507531 Magdala Red Phloxin B 524600 Mag Fura Red483 427659 631Ratio Dye Mg2 Mag Fura 2 369 329508 Ratio Dye Ca2 369 330511 491Ratio Dye Mg2 Mag Fura 5 369 330505 500Ratio Dye Ca2 369 332505 482Ratio Dye Mg2 Mag Indo 1 349 328480 390Ratio Dye Ca2 349 330480 417Ratio Dye Mg2 Magnesium Green506 507531 Magnesium Orange550575 Malachite Green628 Marina Blue362459 Maxilon Brilliant Flavin 10 GFF450495 Maxilon Brilliant Flavin 8 GFF460495 Merocyanin555578 Methoxycoumarin360410 Mitotracker Green FM490516 Mitotracker Orange551576 Mitotracker Red578599 Mitramycin450470 Monobromobimane398490 Monobromobimane mBBr GSH 398500 Monochlorobimane380461 MPS Methyl Green Pyronine Stilbene 364395 NBD466539 NBD Amine450530 Nile Red515 555 559590 640 Nitrobenzoxadidole465 510 650 Noradrenaline340 490 520 Nuclear Fast Red289 530580 Nuclear Yellow365495 Nylosan Brilliant Iavin E8G460510 Oregon Green503522 Oregon Green 488 X494517 Oregon Green 503522 Oregon Green 488490 493514 520 Oregon Green 500497517 Oregon Green 514506526 Pacific Blue405455 Pararosaniline Feulgen 570625 PBFI340 380420 Excitation ratio dye PE Cy5488670 PE Cy7488 755 767 PerCP488675 PerCP Cy5 5488710 PE TexasRed Red 613 488613 Phloxin B Magdala Red 524600 Phorwite AR360430 Phorwite BKL370430 Phorwite Rev380430 Phorwite RPA375430 Phosphine 3R465565 PhotoResist365610 Phycoerythrin B PE 546 565575 Phycoerythrin R PE 565578 PKH26 Sigma 551567 PKH67496520 Chroma PMIA341376 Pontochrome Blue Black535 553605 POPO 1433457 POPO 3533574 PO PRO 1435455 PO PRO 3539567 Primuline410550 Procion Yellow470600 Propidium Iodid PI 305 536 538617 PyMPO412 415561 564 570 Pyrene360387 Pyronine410540 Pyronine B540 590560 650 Pyrozal Brilliant Flavin 7GF365495 QSY 7560 Quinacrine Mustard440510 Red 613 PE TexasRed 488613 Resorufin571 584 585 RH 414532716 Rhod 2552576 Rhodamine550573 Rhodamine 110496 497520 Rhodamine 123507529 Rhodamine 5 GLD470565 Rhodamine 6G525555 Rhodamine B540625 Rhodamine B 200523 557595 Rhodamine B extra550605 Rhodamine BB540580 Rhodamine BG540572 Rhodamine Green502527 Rhodamine Phallicidine558 542575 565 Rhodamine Phalloidine542565 Rhodamine Red570590 Rhodamine WT530555 Rose Bengal525 540550 600 R phycocyanine R phycoerythrin PE 565578 rsGFP473509 red shifted GFP S65T S65A471504 S65C479507 S65L484510 S65T488511 Sapphire GFP395511 SBFI340 380420 Excitation ratio dye Serotonin365 520 540 Sevron Brilliant Red 2B520595 Sevron Brilliant Red 4G500583 Sevron Brilliant Red B530590 Sevron Orange440530 Sevron Yellow L430490 sgBFP 387450 sgBFP super glow BFP 387450 Quantum s SuperGlo GFP AFPs sgGFP 474488 sgGFP super glow GFP 474509 Quantum s SuperGlo GFP AFPs SITS336436 Ion Channels SITS Primuline 395 425450 SITS Stilbene Isothiosulphonic Acid 365460 SNAFL calcein506 535535 620Ratio Dye pH SNAFL 1508 540543 623Ratio Dye pH SNAFL 2514 543546 630Ratio Dye pH SNARF calcein552 574590 629Ratio Dye pH SNARF1576 548635 587Excitation and emission ratio dye Sodium Green506 507532 Na K SpectrumAqua433 53480 55Vysis SpectrumGreen497 30 509 31538 44 524 56Vysis SpectrumOrange559 38 560588 48Vysis Spectrum Red587 587 35612 612 51 SPQ 6 methoxy N 3 sulfopropyl quinolinium 344443 Stilbene335440 Sulphorhodamine B can C520595 Sulphorhodamine G Extra470570 SYTO 11508 510527 530Dye for DNA RNA SYTO 12499 500522 519Dye for DNA RNA SYTO 13488 491509 514Dye for DNA RNA SYTO 14517 521549 547Dye for DNA RNA SYTO 15516 518546 555Dye for DNA RNA SYTO 16488 494518 525Dye for DNA RNA SYTO 17621634 Dye for DNA SYTO 18490 493507 527Dye for DNA RNA SYTO 20512530 Dye for DNA SYTO 21494517 Dye for DNA SYTO 22515535 Dye for DNA SYTO 23499520 Dye for DNA SYTO 24490515 Dye for DNA SYTO 25521556 Dye for DNA SYTO 40420441 Dye for DNA SYTO 41430454 Dye for DNA SYTO 42433460 Dye for DNA SYTO 43436467 Dye for DNA SYTO 44446471 Dye for DNA SYTO 45452484 Dye for DNA SYTO 59622645 Dye for DNA SYTO 60652678