Biofilm细菌生物膜适用医学生

1、1会计学Biofilm细菌生物膜适用医学生细菌生物膜适用医学生“In a paper in Science in 1999, we said 65 percent of all diseases in the developed world are biofilms,” Costerton said. “Now the NIH says 80 percent. ”Biofilms matrix-enclosed microbial accretions/逐渐生长逐渐生长/增加增加 that adhere to biological or non-biological surfaces.on a

2、 contact lenson a spider细菌生物膜广泛存在于自然环境中细菌生物膜广泛存在于自然环境中回肠表面的细菌生物被膜回肠表面的细菌生物被膜人体植入导管表面的细菌生物被膜细菌生物膜广泛存在于自然环境中细菌生物膜广泛存在于自然环境中真菌生物膜细菌生物膜细菌生物膜进入体内的进入体内的可能途径可能途径Biofilms matrix-enclosed microbial accretions/逐渐生长逐渐生长/增加增加 that adhere to biological or non-biological surfaces.古老：fossil record (3.25 billion ye

3、ars ago)Common：a diverse range of organismsArchaea/ 单细胞生物, bacteria，fungiRepresents a protected mode of growth that allows cells to survive in hostile environmentsCan disperse to colonize new niches/可以释放出浮游细菌，生长迅速，是潜在感染因素可以释放出浮游细菌，生长迅速，是潜在感染因素Biofilm 特点总结特点总结Biofilm 的形成过程的形成过程- 体内过程体内过程Biofilm 的形成过程

4、的形成过程- 从菌落角度从菌落角度细菌的初始吸附与细菌的初始吸附与其鞭毛、菌毛有关其鞭毛、菌毛有关Nature Rev Micro 2010像像“铠甲铠甲”一样一样生物膜的结构特点生物膜的结构特点细菌生物膜的结构特点细菌生物膜的结构特点成分成分The Biofilm MatrixNat Rev Micro 2010成分成分原理原理1、EPS的存在可以阻止抗生素作用- 细菌耐药性；2、抗免疫清除：屏蔽作用使吞噬细胞和杀伤细胞及其所分泌的酶不能发挥作用；3、细菌生活微环境改变：比如厌氧菌繁殖、H2S产生、pH改变（口臭、龋齿等）4、Quorum sensing：参阅 , by Bonnie Bas

5、ler (普林斯顿大学) 细菌生物膜与抗性和特点细菌生物膜与抗性和特点细菌生物膜细菌生物膜进入体内的进入体内的可能途径可能途径临床意义临床意义临床意义临床意义Apical periodontitis is a chronic inflammatory disorder of periradicular tissues caused by aetiological agents of endodontic origin. Persistent apical periodontitis occurs when root canal treatment of apical periodontitis

6、 has not adequately eliminated intraradicular infection. Problems that lead to persistent apical periodontitis include: inadequate aseptic control, poor access cavity design, missed canals, inadequate instrumentation, debridement and leaking temporary or permanent restorations. Even when the most st

7、ringent procedures are followed, apical periodontitis may still persist as asymptomatic radiolucencies/ 射线射线可透性可透性, because of the complexity of the root canal system formed by the main and accessory canals, their ramifications and anastomoses where residual infection can persist. P. N. R. Nair (200

8、6) International Endodontic Journal. On the causes of persistent apical periodontitis: a review与口腔的关系与口腔的关系Further, there are extraradicular factors located within the inflamed periapical tissue that can interfere with post-treatment healing of apical periodontitis. The causes have not been well cha

9、racterized, but there are six biological factors that lead to asymptomatic radiolucencies persisting after root canal treatment. (i) intraradicular infection persisting in the complex apical root canal system; (ii) extraradicular infection, generally in the form of periapical actinomycosis; (iii) ex

10、truded root canal filling or other exogenous materials that cause a foreign body reaction; (iv) accumulation of endogenous cholesterol crystals that irritate periapical tissues; (v) true cystic lesions, and (vi) scar tissue healing of the lesion生物被膜的观察研究方法生物被膜的观察研究方法 扫描电镜: Costerton等人在70年代通过扫描电镜观察到了细菌的生物被膜的存在。 激光共聚焦显微镜: 分子生物学方法： Huth et al2008/docs/schultz_eurowound.pdfSEMcoccineutrophilbugThanksAdiosCiao谢谢谢谢Flush your teeth dailyBrush your teeth 3 times a day细菌生物膜细菌生物膜进入体内的进入体内的可能途径可能途径The Biofilm MatrixNat Rev Micro 2010成分成分原理