两种制备方法的紫癜方治疗ITP模型小鼠的药效研究

1、两种制备方法的紫癜方治疗ITP模型小鼠的药效研究         10-08-18 10:38:00     作者：张林 马骥 林淑茹     编辑：studa20【摘要】  观察两种制备方法的紫癜方对ITP模型小鼠的治疗作用。方法采用免疫法建立ITP模型，设立正常组（C）、模型组（M）、醋酸泼尼松组（DC）、血美安组（X）、紫癜水提组（S）和紫癜工艺组（G），利用全自动多物种五分类动物血液分析仪于实验前和实验第14天检测各组

2、小鼠血小板数，实验前、第7天和14天分别记录各组小鼠体重，实验结束记录各组小鼠皮下出血程度。结果造模前各组小鼠体重和血小板计数均无显著性差异。实验第7天，M组小鼠体重低于C组，有下降趋势，但无显著性差异；DC组、X组、S组和G组小鼠体重高于M组，有上升趋势，但无显著性差异；其它各组小鼠体重比较，无显著性差异。实验第14天，M组小鼠体重和血小板计数明显低于C组，较C组出现明显瘀斑，DC组、X组、S组和G组小鼠体重均低于C组，血小板计数明显低于C组；DC组、X组、S组和G组小鼠体重和血小板计数均明显高于M组，DC组、S组和G组小鼠皮肤瘀斑较M组明显减轻，X组小鼠皮肤瘀斑与M组比较无显著性差异；X组

3、、S组和G组小鼠血小板计数明显低于DC组；S组和G组小鼠血小板计数明显高于X组；其它各组小鼠体重和血小板计数比较，无显著性差异。结论紫癜方的两种制备方法对其治疗ITP的药效作用无影响。 【关键词】  特发性血小板减少性紫癜；紫癜方；制备方法；药效研究         QbjectiveTo observe the effects of Zidian prescription extracted by two kinds of process on ITP mice. Methods Using

4、APS to inject into mice to induce the ITP model. Mice were divided into 6 groups: C, M, DC, X, S and G groups. The number of platelet on 7th day and 14th day were measured in mice. Meanwhile the weight on 7th day and 14th day and the degree of subcutaneous bleeding were recorded. ResultsThere is no

5、significant difference in weight and the number of platelet in all groups before the experiment. Compared with the C group, the weight is in down trend in M group on 7th day, the weight and the number of platelet were declined in M group on 14th day, the petechia was more clearly in M group. Compare

6、d with the M group, the weight is up trend in DC,X,S and G groups on 7th day, the weight and the number of platelet are enhanced in DC,X,S and G groups on 14th day, the petechia was decreased in DC, S and G groups significantly. Compared with the DC group, the number of platelet is decreased in X,S

7、and G groups. Compared with the X group, the number of platelet is increased in S and G groups. Conclusion Zidian prescription extracted by two kinds of extraction process has an remedial effect on ITP mouse. There is no significant difference between the two kinds of extraction process.Key words: i

8、diopathic thrombocytopenic purpura; Zidian prescription; extraction process; pharmacodynamic action    特发性血小板减少性紫癜（ITP）是一种常见的出血性疾病。本文从药效学指标比较两种制备方法提取的紫癜方治疗ITP的作用差异，从而明确紫癜方的制备方法对其效用的影响。材料与方法1  材料1.1  动物 SPF级健康BALB/C小鼠，体重为(1822)g，雌雄各半，动物许可证号：SCXK（京）20020003，由北京维通利华实验技术有限公

9、司提供。豚鼠，体重为(250350)g，普通级，46周龄，动物许可证号：SCXK（吉）20030004，由吉林省实验动物质量检测中心提供。1.2  药物 方剂紫癜方：由黄芪25g,制大黄15g,生晒参10g,阿胶25g,桃仁10g,生地10g,白芍20g,仙鹤草15g,当归10g,甘草10g组成。全部药材购自沈阳天益堂药房。    制备方法一（紫癜水提方）：方中药物常规煎煮水提，加水1500ml（药量的10倍量），浸泡30min，煎煮时间为25min，收集药液，取过滤后药物加水1200ml（药量的8倍量），按照前述方法再次煎煮，将两次煎煮后的药

10、液混合浓缩成100%（含生药1g/ml）备用。    制备方法二（紫癜工艺方）：方中大黄、人参5g和白芍用240ml（药量的6倍量）70%乙醇回流提取2次，每次2小时，回收乙醇至无醇味，形成药液一；药渣和黄芪、桃仁、生地、仙鹤草、当归和甘草加水1200ml（药量的10倍量），浸泡30min，煎煮时间为25min，收集药液，取过滤后药物加水900ml（药量的8倍量），按照前述方法再次煎煮，将两次煎煮后的药液混合为药液二，将药液一和药液二合并，浓缩成100%（含生药1g/ml）的浓缩液；人参5g粉碎过100目筛兑入浓缩液；阿胶粉碎成粗粉加热兑入。    醋酸泼尼松片由天津太平洋制药有限公司生产（国药准字H12020809）。将醋酸泼尼松片研成粉末，用生理盐水配成混悬液（0.3g/L）。血美安胶囊由贵州正鑫药业有限公司提供（国药准字Z20044441）。将血美安胶囊中颗粒状内容物用生理盐水配配成混悬液（32g/L）。1.3  试剂 完全弗氏佐剂，SIGMA原装，购自沈阳方隆生物技术有限责任公司，批号：065K891