复方中药复明片对兔视网膜脱离后视网膜组织形态的影响

1、复方中药复明片对兔视网膜脱离后视网膜组织形态的影响         11-03-04 13:49:00     编辑：studa20                   作者：彭清华，刘娉，彭俊，姚小磊，谭涵宇【摘要】  目的：研究复方中药复明片对兔实验性视网膜脱离后视网膜组织形态结构的影

2、响。方法：用有色家兔72只，设正常对照A组、模型B组、西药对照C组、复明片D组，每组9只。B，C，D组制作视网膜脱离模型。造模后第1d下午开始给药，A组与B组均予温开水灌胃，C组予西药混合溶液灌胃，D组予复明片混悬液灌胃，皆5mL/kg，1次/d。造模后7，14，21d观察各组视网膜复位情况，取材后光镜下观察视网膜组织形态学结构变化。结果：复明片能促进脱离视网膜复位,减轻视网膜组织及细胞的形态学损伤，改善视功能。结论：复明片有保护视网膜组织结构的作用。 【关键词】  复明片；益气养阴活血利水法；视网膜脱离模型；组织结构；兔    AstractAIM:

3、To investigate effects of fuming tablets(FMT) on morphology of retinal tissue after retinal detachment(RD) of rabbits. METHODS: Seventytwo rabbits were divided into normal group (group A), model group (group B), western medicine group(group C), FMT group(group D). Each group had 9 rabbits.Group B,C,

4、D were made RD model. After models were made one day they were given medicine. Group A and B were administrated intragastrically with warm water. Group C was administrated intragastrically with western medicine solution. Group D was administrated intragastrically with FMT suspension, once a day. Aft

5、er models were made, the retina recovery and the changes of morphology of retinal tissue on the 7th, 14th, and 21st day, were observed with microscope. RESULTS: FMT can promote the retinal reattachment, reduce the morphology damage of retina cells and tissue and improve sight. CONCLUSIONS: FMT has e

6、ffects of protecting retinal tissue structureKEYWORDS: fuming tablets; method of qibenefitting, yinfostering, and blood invigorating and diuresisactivating; retinal detachment model; tissue structure; rabbit0引言        益气养阴活血利水法能促进视网膜脱离(retinal detachment, RD)术后患者视网

7、膜的复位，提高其术后视功能1。为了探讨其作用机制，我们观察具有益气养阴活血利水作用的复明片对兔实验性视网膜脱离后视网膜组织形态结构的影响如下。    1材料和方法    1.1材料 成年健康有色家兔72只，雌雄各半，由湖南中医药大学实验动物中心提供。复明片由黄芪、生地、茯苓、车前仁、地龙等益气养阴活血利水中药按现代制剂制备工艺制成，由湖南中医药大学第一附属医院药剂科提供。使用时研成粉末，温开水混合成浓度为100g/L的混悬液。温开水混合成每升水含VitB 1276mg，ATP 1102mg，维脑路通734mg的溶液。

8、0;   1.2方法 造模2后1d下午开始给药，A正常对照组与B模型对照组均予温开水灌胃，C西药对照组予西药混合溶液灌胃，D复明片治疗组予复明片混悬液灌胃，皆5mL/kg，1次/d。造模后7，14，21d，各小组取对应兔6眼，用30g/L戊巴比妥1mL/kg耳缘静脉注射麻醉兔，摘除眼球，生理盐水冲洗干净。在冰生理盐水中，沿角巩缘剪开眼球达360°，弃角膜、晶状体、玻璃体，保留视网膜连同脉络膜巩膜组织。取各组对应编号中的3眼置于40g/L多聚甲醛固定，12h后修片，切取颞侧脱离部位视网膜（正常对照组切取相应部位），再置于新的40g/L多聚甲醛固定液，石蜡切

9、片，HE染色。光镜下观察视网膜组织病理形态学改变。    2结果    2.1术后7d病理形态学 正常对照组视网膜组织各层结构清晰、完整，排列整齐。光感受器细胞(photoreceptor cells, PRC)与视网膜色素上皮细胞(retinal pigment epithelial cells, RPE cell)连接紧密，形态正常，无变性。内、外核层及神经节细胞(retinal ganglion cell, RGC)数量和排列正常，无空泡样变性，神经纤维层清晰。Müller细胞框架纤维结构完整（图1）。模型对

10、照组局部有浆液性扁平RD 3眼，裂孔处出现视网膜下纤维增生2眼。所有眼PRC与RPE cell结合疏松，视网膜不同层间均可见空泡样变性，其内可见少量有色素的吞噬细胞，PRC层结构排列紊乱，缺失变薄，细胞数目明显减少，大量空泡样变性，外节几乎全部变性脱落；内外核层、网状层结构紊乱，细胞变大、水肿、空泡样变，有核周空泡和核固缩；RGC部分水肿，神经纤维层出现空泡；部分Müller细胞纤维支架消失。PRC层和RPE cell层间有浆液性渗出液，其中RPE cell肥大、增生、游离，少量淋巴样细胞浸润。裂孔处出现视网膜下纤维增生眼脉络膜组织水肿2眼，纤维增生，原裂孔周围感光细胞(receptor cell, RC)外节脱落（图2A）。西药对照组视网膜已复位1眼，局部有浆液性扁平RD 2眼，