常见酶切位点与保护碱基

1、不同内切酶对识别位点以外最少保护碱基数目的要求不同内切酶对识别位点以外最少保护碱基数目的要求酶宜核甘酸序列切割率孑n久口i且又妇/2hr20hrAccIGGTCGACC00CGGTCGACCG00CCGGTCGACCGG00AfllllCACATGTG00CCACATGTGG>90>90CCCACATGTGGG>90>90-一AscIGGCGCGCC>90>90AGGCGCGCCT>90>90-.TTGGCGCGCCAA>90>90】JAvalCCCCGGGG50>90111I'|1,1了fCCCCCGGGGG>9

2、0>90>9011n1.*:JI!TCCCCCGGGGGA>90BamHICGGATCCG1z-1025CGGGATCCCG>90>90CGCGGATCCGCG>90>90BglllCAGATCTG00GAAGATCTTC,-"I75>90GGAAGATCTTCC25>90BssHIIGGCGCGCC00AGGCGCGCCT00TTGGCGCGCCAA50>90BstEllGGGT(A/T)ACCC010BstXIAACTGCAGAACCAATGCATTGG00AAAACTGCAGCCAATGCATTGGAA2550CTGC

3、AGAACCAATGCATTGGATGCAT25>90ClalCATCGATG00GATCGATC00CCATCGATGG>90>90CCCATCGATGGG5050EcoRIGGAATTCC>90>90CGGAATTCCG>90>90CCGGAATTCCGG>90>90HaelllGGGGCCCC>90>90AGCGGCCGCT>90>90TTGCGGCCGCAA>90>90HindlllCAAGCTTGCCAAGCTTGGCCCAAGCTTGGG00100075KpnlGGGTACCC00GGGGTA

4、CCCC>90>90CGGGGTACCCCG>90>90MlulGACGCGTC00CGACGCGTCG2550NcolCCCATGGG00CATGCCATGGCATG5075NdelCCATATGG00CCCATATGGG00CGCCATATGGCG0°iGGGIIICAIAIGAAACCC00GGAATTCCATATGGAATTCC75>90GGGAATTCCATATGGAATTCCC75>90NhelGGCTAGCC00CGGCTAGCCG1025CTAGCTAGCTAG1050切割率%酶寡核苜酸序列2hr20hrNotlTTGCGGCCGC

5、AA00AIIIGCGGCCGCIIIAjs-Z11010AAATATGCGGCCGCTATAAA1010ATAAGAATGCGGCCGCTAAACTAT5if：2590AAGGAAAAAAGCGGCCGCAAAAGGAAAA25>90NsiliTGCATGCATGCA10>90CCAATGCATTGGTTCTGCAGTT>90>90PaclTTAATTAA00GTTAATTAAC025CCTTAATTAAGG0>90PmelGIIIAAAC00GGIIIAAACC025GGGIIIAAACCC050AGCIIIGIIIAAACGGCGCGCCGG75>90

6、PstlGCTGCAGC00TGCACTGCAGTGCA1010AACTGCAGAACCAATGCATTGG>90>90AAAACTGCAGCCAATGCATTGGAA>90>90CTGCAGAACCAATGCATTGGATGCAT00PvuICCGATCGGATCGATCGATTCGCGATCGCGA010002510SacICGAGCTCG1010SacIIGCCGCGGC00TCCCCGCGGGGA50>90SallGTCGACGTCAAAAGGCCATAGCGGCCGC00GCGTCGACGTCTTGGCCATAGCGGCCGCG1050G1075ACGC

7、GTCGACGTCGGCCATAGCGGCCGCGGAAScaIGAGTACTC1025AAAAGIACIIII7575一.SmaICCCGGG010CCCCGGGG010CCCCCGGGGG1050TCCCCCGGGGGA>90>90SpeIGACTAGTC10>90GGACTAGTCC10>90CGGACTAGTCCG050CTAGACTAGTCTAG050SphIGGCATGCC00CATGCATGCATG025ACATGCATGCATGT1050StuIAAGGCCTT5iy*。/>90>90GAAGGCCTTC>90>90AAAAGGCCIIII>90>90XbaI-CTCTAGAG00GCTCTAGAGC>90>90TGCTCTAGAGCA75>90CTAGTCTAGACTAG75>90Xho