胶体金试纸条技术

1、The colloidal gold strip testing technology胶体金试纸条检测技术胶体金试纸条检测技术IntroductionThe colloidal gold strip diagnosis is developed by colloidal gold immune chromatography technology. The technology was established on the basis of the immune infiltration technology in the early 90 s. It is a simple and rapid

2、 immunological detection technology. The first to use it to detect human chorionic gonadotropin (HCG)and hepatitis B virus surface antigen(HBsAg),and so on.Colloidal godIt is gold chloride acid (HAuCl4) hydrosol, gold chloride acid can aggregat into a certain size of gold particles under the action

3、of reducing agent and become a stable colloidal state by electrostatic interactions.The quality of the colloidal gold standard:Good quality colloidal gold: the solution is red, colloidal gold particle is spherical, uniform size, no edges and corners.Poor quality colloidal gold: the solution is purpl

4、e, size is differ, a variety of shapes.The principle of colloidal gold labeling: colloidal gold is negatively charged in alkaline conditions. Therefore, it can form a solid combination with protein molecules positively charged groups by electrostatic attraction.Immunogold labeling technique: it is a

5、 new type of immune markers technology. As a tracer marker, colloidal gold is applied to the antigen-antibody reactionThe principle of the colloidal gold strip diagnosis technologyUsing the nitrocellulose membrane as the carrier and making use of the capillary effect of microporous membrane, we add

6、liquid on one side of the membrane, then the liquid move to the other end slowly. After the combination of antigen-antibody, we can take advantage of the colloidal gold color reaction to detect antigen or antibody.It is the most commonly method to detect antigen by using double clamp methodholding w

7、ire（T）The line of control line (C)The line of control line (C)Colloidal gold padabsorbent paperSample padNC membraneTomographic directionPVC baseplateStrip structuremeasure When measuring, we make the bottom of test paper strip immerse in the liquid samples, then the bottom absorbs liquid and move t

8、owards the upper end. when the liquid flow through the C , the immune gold complex dissolve on the strip.If there are a test specific antigen in the specimen, it can be combined with immune antibodies in the gold complexes. when the antigen-antibody complex flows to the test area, it can be captured by solid phase antibody and show reaction red line on the membrane.When excess immune gold compounds flow to the reference area and combine with solid-phase anti-mouse IgG(monoclonal antibodies in the immune gold compound