降解菌短密木霉对土壤酶活性的影响

1、降解菌短密木霉对土壤酶活性的影响降解菌短密木霉对土壤酶活性的影响8023202103-0009-04Effects of Trichoderma brevicompactum on Soil Enzyme ActivityLIU Qing1， DENG Airong1*， DENG Shilin1， BAO Tong1， LIU Wei21.College of Forestry， Northeast Forestry University， Harbin 150040; 2.Huludao Forestry Development Service Center， Huludao 125000

2、Abstract：In order to investigate the effects of Trichoderma brevicompactum and the interaction of Trichoderma brevicompactum， soybean and imazethapyr on the activities of sucrase and catalase in soil， 3.5-dinitrosalicylic acid method and potassium permanganate titration were used to determine the ac

3、tivities of sucrose and catalase in soil respectively. The results showed that， when Trichoderma brevicompactum was added to the soil， in 5-20 days， sucrase activity showed a trend of activation with the highest activity of 53.85 mg/kg in 10 days and the activation rate of 169.94%. Catalase activity

4、 showed a trend of activation-inhibition-recovery. The highest catalase activity was 2.47 mg/g and the activation rate was 5.71%. Then it was inhibited. The lowest catalase activity was 2.30 mg/g and the highest inhibition rate was 17.86%. After planting soybean， adding Trichoderma brevicompactum an

5、d 100 mg/kg imazethapyr， soil sucrose activity showed the trend of activation-recovery-activation-inhibition. The highest sucrase activity was 60.09 mg/kg in 10 days， the activation rate was 201.21%， and the sucrase activity was in habited in 20 days with the highest sucrase activity of 68.12 mg/kg

6、and the inhibition rate of 62.43%. Soil catalase activity showed a trend of inhibition-recovery-activation， the lowest activity was 2.43 mg/g in 20 days， the highest inhibition rate was 17.98%， the highest activity was 2.70 mg/g in 40 days， and the highest activation rate was 9.46%.Keywords：Trichode

7、rma brevicompactum; sucrase; catalase0引言土壤酶是土壤生态系统代谢的重要驱动力，土壤中进行化学和生物反应是在酶的催化下进行的。土壤微生物和土壤酶活性改变作为检测土壤质量改变的指标之一，反映了土壤生态系统中微生物参與物质转化和循环的能力，因此土壤酶受到人们越来越多的关注1-2。土壤酶参加土壤中全部的生化反应，在物质转化、能量代谢和污染土壤修复等过程中发挥着重要作用3。同时土壤酶作为一项生态毒理指标，常用于评价土壤健康及生态环境风险4。有讨论说明，土壤酶可参加有机化合物的降解，因此，它能在治理污染土壤方面得到应用5。重复使用一种或几种类似化学结构的农药，可能导

8、致农药的快速降解，很快丧失药效，土壤酶参加上述过程，被称为“激活生物降解6。土壤酶降解土壤中的有机化合物的机理，一般认为涉及几个过程：降解、聚合、合成和偶联的酶促反应，形成腐殖质复合体，从而削减土壤中有害有机物的聚积。 3结论与商量本讨论说明：在土壤中加入短密木霉后，土壤蔗糖酶活性呈现出激活恢复抑制的趋势，且与对比组差异显著P0.05。过氧化氢酶活性呈现激活抑制恢复的趋势，且与对比组差异显著P0.05。依据课题组前期讨论结果，加入短密木霉后，10 d时短密木霉数量最多，其后占比渐渐下降，且土壤中的真菌总数量恢复到对比水平22。上述结果与短密木霉在土壤中的数量改变有直接关系。这与陈建爱等23的讨论结果基本一致，黃绿木霉T1010制剂处理日光温室番茄连作土壤，可有效提高土壤酶