前列腺素 E2作用机制 - Medchemexpress - MCE中国

1、Product Data SheetProstaglandin E2Cat. No.: HY-101952CAS No.: 363-24-6分式: CHO分量: 352.47作靶点: Prostaglandin Receptor; Endogenous Metabolite作通路: GPCR/G Protein; Metabolic Enzyme/Protease储存式: Powder -20C 3 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (283.71 mM)* means soluble, bu

2、t saturation unknown.SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 2.8371 mL 14.1856 mL 28.3712 mL5 mM 0.5674 mL 2.8371 mL 5.6742 mL10 mM 0.2837 mL 1.4186 mL 2.8371 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使

3、。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (7.09 mM); Clear solution此案可获得 2.5 mg/mL (7.0

4、9 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 25.0 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (7.09 mM); Clear solution此案可获得 2.5 mg/mL (7.09 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例

5、，取 100 L 25.0 mg/mL 的澄均匀。DMSO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合BIOLOGICAL ACTIVITY物活性 Prostaglandin E2和炎症的调节。种激素样物质，参与泛的体功能，如平滑肌的收缩和松弛，管的扩张和收缩，压的控制IC & Target EP2 Receptor Human Endogenous Metabolite体外研究 PGE2 shows inhibition of IL 2 production in the mixture of irradiated and nonirradiated T lympho

6、cytes. PGE2 (0.1-10 M) dose-dependently inhibits the production of IL 2. PGE2 acts during the inductive phase of activation of suppressorcells. Preincubation of T lymphocytes with PGE2 induces cells that suppress IL 2 production and PHA proliferation1.体内研究 PGE2 (0.3 g/k, i.p.) significantly reduces

7、the number of peritoneab macrophages undergoing phagocytosis of themethacrybate microbeads in rats2. PGE2 (0.1 mg/min, i.a.) increases renal blood flow. PGE2 produces a biphasic change in renal vascular resistance, vasodilatation starts at 0.01 mg/min and is maximal at about 3 mg/min, while atthe hi

8、ghest dose used (20 mg/min) PGE2 induces renal vasoconstriction3.PROTOCOLCell Assay 1 Lymphocytes in CM (1106 cells/mL) are ditributed in microculture plates (100 L) in triplicate in the presence ofPGE-treated T cells or medium-treated T cells and stimulated with PHA-P at various mitogenic doses. Af

9、ter 72 hr,cultures are pulsed with 1 Ci 3Hthymidine per well (specific activity 5 Ci/mM) for 16 to 18 hr, collected withamicroprecipltator, dried, and counted in a liquid scintillation counter.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Male

10、Sprague Dawley rats (200-250 g) are used throughout the study. For 3 consecutive days rats in the experimentalAdministration 2 groups receive a daily intraperitoneal injection of either PGE2 (0.3 g/kg body weight (BW), the prostaglandininhibitor mecbofenamate (10 mg/kg BW) or the prostaglandin precu

11、rsor arachidonic acid (0.3 g/ kg BW). Todetermine whether or not 0.3 g/kg BW of a fatty acid produces nonspecific effects, the biologically inactive fattyacid 11, 14, 17-eicosatrienoic acid is also administered to a group of rats. Rats in the control group receive anequivalent volume (2.0 mL/kg BW)

12、of the vehicle. On the third day, 3 mL of a suspension containing 1.2106fluorescent methacrylate microbeads/mL of PBS are injected intraperitoneally (ip) into each rat. Six hours later allanimals are given ip a regular dose of their respective treatment. Peritoneal exudate cells are harvested 19-22

13、hrlater.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Front Microbiol. 20 May 2020. J Cell Commun Signal. 2020 Jan 10. J Pediatr Surg. 2019 Feb 24. pii: S0022-3468(19)30008-9.See more customer validations on HYPERLINK www.MedChemE www.MedC

14、hemEPage 2 of 3 www.MedChemEREFERENCES1. Chouaib S, et al. The mechanisms of inhibition of human IL 2 production. II. PGE2 induction of suppressor T lymphocytes. J Immunol. 1984Apr;132(4):1851-7.2. Fernandez-Repollet E, et al. In vivo effects of prostaglandin E2 and arachidonic acid on phagocytosis of fluorescent methacrylate microbeads by ratperitoneal macrophages. J Histochem Cytochem. 1982 May;30(5):466-70.3. Haylor J, et al. Renal vasodilator activity of prostaglandin E2 in the rat anaesthetized with pentobarbitone. Br J Pharmacol. 1982 May;76(1):131-7