Phenol-Red-sodium-salt-Phenolsulfonephthalein-sodium-salt-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEPhenol Red sodium saltCat. No.: HY-D0169ACAS No.: 34487-61-1Synonyms: Phenolsulfonephthalein sodium salt分式: CHNaOS分量: 376.36作靶点: Others作通路: Others储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect f

2、romlight)溶解性数据体外实验 H2O : 100 mg/mL (265.70 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6570 mL 13.2852 mL 26.5703 mL5 mM 0.5314 mL 2.6570 mL 5.3141 mL10 mM 0.2657 mL 1.3285 mL 2.6570 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY

3、物活性 Phenol Red sodium salt溶性的pH指剂，在pH 6.8（ ）8.2（红）范围内。体外研究Phenol Red sodium salt is commonly employed as a cell tissue culture pH marker. Under normal conditions,phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminantswill cause a change in pH resul

4、ting in a change in indicator color. Phenol red bears a structural resemblanceto some nonsteroidal estrogens and has significant estrogenic activity at the concentrations (15-45 M) at1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEwhich it is found in tissue culture media. Phenol red binds to the e

5、strogen receptor of MCF-7 human breastcancer cells and stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in adose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breastcancer cells 1. Phenol red is a simple, rapid and inexp

6、ensive method for the measurement of hydrogenperoxide produced by cells in culture. The assay is based on the horseradish peroxidase-mediated oxidationof phenol red by hydrogen peroxide which results in the formation of a compound demonstrating increasedabsorbance at 610 nm 2.PROTOCOLCell Assay 12 P

7、henol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seededinto T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks

8、 are harvested andcounted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, whichcontains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 M),tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell numbe

9、r is monitored as a functionof time 1. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Berthois Y, et al. Phenol red in tissue culture media is a weak estro

10、gen: implications concerning the study of estrogen-responsive cells inculture. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500.2. Pick E, et al. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods.1980;38(1-2):161-70.McePdfHeightCaution: Product has not been fully valid