Liothyronine-sodium-Triiodothyronine-sodium-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemELiothyronine sodiumCat. No.: HY-A0070CAS No.: 55-06-1Synonyms: Triiodothyronine sodium; 3,3,5-Triiodo-L-thyronine sodium; T3sodium分式: CHINNaO分量: 672.96作靶点: Thyroid Hormone Receptor作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn

2、solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 42 mg/mL (62.41 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.4860 mL 7.4299 mL 14.8597 mL5 mM 0.2972 mL 1.4860 mL 2.9719 mL10 mM 0.1486 mL 0.7430 mL 1.4860 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和

3、期限。体内实验请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (3.09 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)1/2 Master of Sma

4、ll Molecules 您边的抑制剂师www.MedChemESolubility: 2.08 mg/mL (3.09 mM); Clear solution3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.08 mg/mL (3.09 mM); Clear solutionBIOLOGICAL ACTIVITY物活性 Liothyronine sodium种有效的甲状腺激素，可以结合并激活 1 甲状腺激素受体 (TR1)。IC50 & Target TR1 1 2体外研究 Liothyronine (T3, 100 nM) stimulate

5、s the proliferation of hepatocarcinema cells in which TR1 isoverexpressed 1. Liothyronine binds to human 1 thyroid hormone receptor (hTR1), and change itsconformation. Liothyronine promotes growth, induces differentiation and regualtes metabolic effects 2.PROTOCOLCell Assay 1 Thyroid hormone deplete

6、d (Td) serum is prepared. The growth of hepatocarcinoma cells in methylcellulose isperformed. To determine the effect of Liothyronine (T3) on the growth of cells, cells are plated at a density of3 104 cells/60 mm dish on day 0, and incubated in medium containing 5% regular serum, 5% Td or 5% Tdand 1

7、00 nM T3. The colony formation in methylcellulose is scored 3 weeks after initial plating 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Lin KH, et al. Stimulation of proliferation by 3,3,5-triiodo-L-thyronine in poorly differentiated hu

8、man hepatocarcinoma cellsoverexpressing beta 1 thyroid hormone receptor. Cancer Lett. 1994 Oct 14;85(2):189-94.2. Bhat MK, et al. Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3,5-triiodo-L-thyronine.Biochem Biophys Res Commun. 1993 Aug 31;195(1):385-92.McePdfHeightCaution: Product has not been fully validated for medical applications