Sal003 - Phosphatase 抑制剂 - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemESal003Cat. No.: HY-15969CAS No.: 1164470-53-4分式: CHClNOS分量: 463.21作靶点: Phosphatase作通路: Metabolic Enzyme/Protease储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 100 mg/mL (215.88 mM)* means

2、soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.1588 mL 10.7942 mL 21.5885 mL5 mM 0.4318 mL 2.1588 mL 4.3177 mL10 mM 0.2159 mL 1.0794 mL 2.1588 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您

3、现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (5.40 mM); Suspended solution; Need ultrasonic2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (5.40 mM); Clear solution1/3 Master of Small Molecules 您边的抑制剂师ww

4、w.MedChemEBIOLOGICAL ACTIVITY物活性 Sal003效、特异性的细胞渗透性 eIF2 磷酸酶抑制剂。Sal003 salubrinal 的衍物 1。IC50 & Target eIF2 phosphatase 1体外研究 Sal003 (20 M; 1-12 hours) sharply increases eIF2 phosphorylation in mouse embryonic fibroblasts (MEFs)2.Eukaryotic translation initiation factor 2 (eIF2) phosphorylation by Sal

5、003 (10 M; 1 hour) enhancessubtilase cytotoxin (SubAB)-induced apoptotic signaling 1.Sal003 promotes eIF2 phosphorylation leads to impairment of synaptic plasticity and memory 1.Apoptosis Analysis 2Cell Line: HeLa cellsConcentration: 10 MIncubation Time: 1 hourResult: Phosphorylated eIF2 and thus en

6、hanced SubAB-induced apoptotic signaling.Western Blot Analysis 1Cell Line: Mouse embryonic fibroblasts (MEFs)Concentration: 20 MIncubation Time: 1 hour, 3 hours, 6 hours, 12 hoursResult: Sharply increased eIF2 phosphorylation in mouse MEFs.体内研究 Sal003 (20m; intrahippocampal injection; 8 minutes) imp

7、airs contextual memory in vivo 1.Animal Model: Rats (300-325g) 1Dosage: 20 MAdministration: Intrahippocampal injection; 8 minutesResult: Impaired contextual memory.REFERENCES1. Costa-Mattioli M, et al. eIF2alpha phosphorylation bidirectionally regulates the switch from short- to long-term synaptic p

8、lasticity andmemory. Cell. 2007 Apr 6;129(1):195-206.2. Yahiro K, et al. Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulumkinase-dependent proteasome pathway in HeLa cells. Infect Immun. 2012 May;80(5):1803-14.2/3 Master of Small Molecules 您边的抑制剂师www.MedChemEMcePdfHeightCaution: Product has not been fully validated for medical application