Dihydrofluorescein-diacetate - Dye Reagent - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDihydrofluorescein diacetateCat. No.: HY-101893CAS No.: 35340-49-9分式: CHO分量: 418.4作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 106.5 mg/mL (254.54 mM)* means solubl

2、e, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.3901 mL 11.9503 mL 23.9006 mL5 mM 0.4780 mL 2.3901 mL 4.7801 mL10 mM 0.2390 mL 1.1950 mL 2.3901 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Dihydrofluorescein diacetate于细胞系统和细胞模型中测量氧化应激的荧光探针。体外研究D

3、ihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a betterfluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizingspecies. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures,

4、 consistent withmitochondria, in reoxygenated endothelium 1. Dihydrofluorescein diacetate is able to detect the presence of1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filamentswhich corresponded in all

5、details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROSreleased in the matrix 2. Dihydrofluorescein diacetate could be an useful and quantitative method formeasuring the oxidative potential of nanoparticle-treated cells 3.PROTOCOLCell Assay Dihydrofluorescein diacetate is us

6、ed at a final concentration of 5 M for the cell-free studies. The excitationwavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emissionwavelengths of the confocal microscope. All fluorescence measurements are performed on roomtemperature solutions. Human umbil

7、ical vein endothelium at first passage are cultured to confluence on 48well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells.Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loadedwith fluorescent probes to se

8、rve as controls. Fluorescence measurements are made on a fluorescentplatereader every 10 min at Ex 488 nm and Em 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). TheEm 510 nm and Em 538 nm values are added together 1.MCE has not independently confirmed the accuracy of these methods. They are for ref

9、erence only.户使本产品发表的科研献 Food Hydrocolloid. 2019 Feb.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2,7-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2,7-di

10、chlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free RadicBiol Med. 1999 Jul;27(1-2):146-59.2. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003Oct;120(4):319-25.3. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment ofnanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.McePdfHeightCaution: Product has not been fu