SIRT7-inhibitor-97491-DataSheet-生命科学试剂-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•Agonists•ScreeningLibrarieswww.MedChemESIRT7inhibitor97491Cat.No.:HY-135899CASNo.:1807758-81-1分⼦式:C₁₅H₁₂ClN₃O分⼦量:285.73作⽤靶点:Sirtuin;Apoptosis作⽤通路:CellCycle/DNADamage;Epigenetics;Apoptosis储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:500mg/mL(1749.90mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.4998mL17.4990mL34.9981mL5mM0.7000mL3.4998mL6.9996mL10mM0.3500mL1.7499mL3.4998mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存⽅式和期限。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的⼯作液，建议您现⽤现配，当天使⽤；澄的储备液可以根据储存条件，适当保存；以下溶剂前的百分⽐指该溶剂在您配制终溶液中的体积占⽐)：1.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥4.17mg/mL(14.59mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性SIRT7inhibitor97491⼀种有效的SIRT7抑制剂，IC50为325nM，SIRT7inhibitor97491降低SIRT7去⼄酰化酶活性，这种作⽤存在剂量依赖性。SIRT7inhibitor97491通过在K373/382处进⾏⼄酰化来提⾼p53的1/2MasterofSmallMolecules—您⾝边的抑制剂⼤师www.MedChemE稳定性，从⽽防⽌肿瘤进展。SIRT7inhibitor97491通过caspase途径促进凋亡(apoptosis)[1]。IC50&TargetSIRT7325nM(IC50)体外研究SIRT7inhibitor97491(1-10μM)reducescellgrowthinMES-SAcells,withoutcausingcytotoxicityinHEK293cells[1].CellProliferationAssay[1]CellLine:HumanuterinesarcomaMES-SAcellsConcentration:1,5,10μMIncubationTime:72hoursResult:Ledtomorethan50%decreaseincellproliferationatconcentrationsof5and10μM.CellCytotoxicityAssay[1]CellLine:HumanembryonickidneycelllineHEK293cellsConcentration:1,5,10μMIncubationTime:24hoursResult:HEK293cellswerealmostunaffected.体内研究SIRT7inhibitor97491(2mg/kg;intraperitoneallyinjected;for3weeks,exceptonweekends)inhibitstumorgrowthinxenograftmice[1].AnimalModel:Balb/cnudemice(18-20g;6-8weeksold)withMES-SAcells[1]Dosage:2mg/kgAdministration:Intraperitoneallyinjected;for3weeks,exceptonweekends.Result:Inhibitedcancergrowthinvivo.REFERENCES[1].Ji-HyeKim,etal.IdentificationofaNovelSIRT7InhibitorasAnticancerDrugCandidate.BiochemBiophysResCommun.2019Jan8;508(2):451-457.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuse