埃希菌属均课件

MedicalMicrobiologyDepartmentofMicrobiology,HMUDepartmentofMicrobiology,HarbinMedicalUniversity第二篇致病性细菌第14章埃希菌属Escherichia微生物学教研室张文莉DepartmentofMicrobiology,HarbinMedicalUniversity教学大纲熟悉肠道杆菌的共同特征及分类致病性大肠埃希菌的种类和致病机制掌握大肠埃希菌的卫生细菌学检查及其意义了解大肠埃希菌的微生物学检查法DepartmentofMicrobiology,HarbinMedicalUniversity形态染色0.4-0.7µm1-3µm短杆菌革兰染色阴性有鞭毛有菌毛DepartmentofMicrobiology,HarbinMedicalUniversityDepartmentofMicrobiology,HarbinMedicalUniversity培养特性与生化反应

兼性厌氧营养要求不高生化反应活泼，发酵多种糖类绝大多数发酵乳糖IMViC试验结果为++--DepartmentofMicrobiology,HarbinMedicalUniversity大肠埃希菌于普通营养平板培养生长的菌落

DepartmentofMicrobiology,HarbinMedicalUniversity复杂的抗原构造DepartmentofMicrobiology,HarbinMedicalUniversity所致疾病肠道外感染泌尿道感染最多见

化脓性感染腹泻DepartmentofMicrobiology,HarbinMedicalUniversity肠产毒素型大肠埃希菌（ETEC）致病物质肠毒素菌毛粘附素内毒素K抗原所致疾病5岁以下婴幼儿腹泻旅游者腹泻DepartmentofMicrobiology,HarbinMedicalUniversityDepartmentofMicrobiology,HarbinMedicalUniversityDepartmentofMicrobiology,HarbinMedicalUniversityDepartmentofMicrobiology,HarbinMedicalUniversity肠侵袭型大肠埃希菌（EIEC）致病物质不产肠毒素，侵袭结肠粘膜上皮细胞所致疾病主要侵犯较大儿童和成人腹泻呈脓血便，有里急后重诊断EIEC无动力、生化反应和抗原结构DepartmentofMicrobiology,HarbinMedicalUniversity肠致病型大肠埃希菌（EPEC

）致病物质不产生肠毒素及其他外毒素无侵袭力所致疾病婴幼儿腹泻DepartmentofMicrobiology,HarbinMedicalUniversity肠出血型大肠埃希菌（EHEC）亦称为vero毒素大肠埃希菌致病物质菌毛：粘附作用毒素：vero毒素所致疾病出血性结肠炎溶血性尿毒综合征DepartmentofMicrobiology,HarbinMedicalUniversityThe“lifecycle”ofE.coliO157:H7

5-10%prevalenceinanimals40%prevalenceinfarmsDepartmentofMicrobiology,HarbinMedicalUniversity

肠集聚型大肠埃希菌EAggEC致病物质毒素粘附素所致疾病引起婴儿持续性腹泻，脱水，偶有血便DepartmentofMicrobiology,HarbinMedicalUniversity微生物学检查法标本分离培养与鉴定肠道外感染肠道内感染DepartmentofMicrobiology,HarbinMedicalUniversity卫生细菌学检查大肠菌群指数每1000(g)ml水中大肠菌群数大肠菌群细菌总数每1ml(g)样品中细菌总数我国卫生标准大肠菌群细菌总数DepartmentofMicrobiology,Ha