Citric-acid-monohydrate-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECitricacidmonohydrateCat.No.:HY-N1428ACASNo.:5949-29-1分⼦式:C₆H₁₀O₈分⼦量:210.14作⽤靶点:Antibiotic;EndogenousMetabolite作⽤通路:Anti-infection;MetabolicEnzyme/Protease储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1monthBIOLOGICALACTIVITY⽣物活性Citricacidmonohydrate⼀种天然防腐剂和⾷品酸味增强剂。Citricacidmonohydrate诱导细胞凋亡(apoptosis)和细胞周期停滞在G2/M期和S期。Citricacidmonohydrate通过降低抗氧化酶活性引起肝脏的氧化损伤。Citricacidmonohydrate对⼩⿏造成肾毒性[1][2][3]。IC50&TargetHumanEndogenousMetabolite体外研究Citricacidmonohydrate(0-12.5mM;24h)showsantiproliferativeactivityinadosedependentmanner[3].Citricacidmonohydrate(12.5mM;72h)inducesapoptosisandcellcyclearrestatG2/MphaseandSphaseinadosedependentmanner[3].Citricacidmonohydrate(12.5mM;48h)increasestheexpressionofFAS,BAX,BID,AIF,EndoG,cytochromec,PARP,GADD153,GRP78andcaspase-3,-8,-9,anddecreasesofBCL-2andBCL-Xl[3].CellViabilityAssay[3]CellLine:HaCaTcellsConcentration:0,2.5,5,7.5,10,12.5mMIncubationTime:24hResult:Inhibitedthecellviabilityinadosedependentmanner.CellCycleAnalysis[3]1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECellLine:HaCaTcellsConcentration:12.5mMIncubationTime:0,12,24,48,72hResult:InducedapoptosisandcellcyclearrestatG2/MphaseandSphaseinadosedependentmanner.WesternBlotAnalysis[3]CellLine:HaCaTcellsConcentration:12.5mMIncubationTime:12,24,48hResult:IncreasedtheexpressionofFAS,BAX,BID,AIF,EndoG,cytochromec,PARP,GADD153,GRP78andcaspase-3,-8,-9,anddecreasedofBCL-2andBCL-Xl.体内研究Citricacidmonohydrate(120,240,and480mg/kg;i.p.)significantlydecreasesGSH-PxactivityandinducesanincreaseintheMDA(malonyldialdehyde)levelsinmouseliver[1].Citricacidmonohydrate(120,240,and480mg/kg;i.p.)inducesapoptosisbyincreasescaspase-3activityinadose-dependentmannerinmousehepatocytes[1].Citricacidmonohydrate(120,240,and480mg/kg;i.p.;weeklyfor3weeks)causesrenaltoxicityinmice[2].AnimalModel:20gmaleKunmingmice[2]Dosage:120,240,480mg/kgAdministration:I.p.;weeklyfor3weeksResult:T-SODandGSH-Pxactivitiesinthetreatedgroupsdecreasedwithincreasingdosesofcitricacid,NOSactivitytendedtoincrease,andH2O2andMDAcontentsgraduallydecreased.户使⽤本产品发表的科研⽂献•NewJChem.03Aug2022.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].ChenX,etal.Studyoninjuryeffectoffoodadditivecitricacidonlivertissueinmice.Cytotechnology.2014Mar;66(2):275-82.[2].ChenX,LvQ,LiuY,DengW.Effectsofthefoodadditive,citricacid,onkidneycellsofmice.BiotechHistochem.2015Jan;90(1):38-44.[3].YingTH,etal.Citricacidinducescell-cyclearrestandapoptosisofhumanimmortalizedkeratinocytecellline(HaCaT)viacaspase-andmitochondrial-dependentsignalingpathways.AnticancerRes.2013Oct;33(10):4411-20.McePdfHeightCaution:Producthasnotbeenfullyvalidat