Pitstop-2-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEPitstop2Cat.No.:HY-115604CASNo.:1419320-73-2分⼦式:C₂₀H₁₃BrN₂O₃S₂分⼦量:473.36作⽤靶点:Apoptosis作⽤通路:Apoptosis储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:62.5mg/mL(132.03mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.1126mL10.5628mL21.1256mL5mM0.4225mL2.1126mL4.2251mL10mM0.2113mL1.0563mL2.1126mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Pitstop2⼀种⽹格蛋⽩抑制剂，可通过与⽹格蛋⽩的末端域相关联来抑制⽹格蛋⽩介导的内吞作⽤(CME)。Pitstop2有潜⼒⽤于抗癌的研究。体外研究Pitstop2(20-40μM;30min)inhibitstheendocytosisoftransferrininJ774A.1macrophagesanddoesnotcompromisecellviability[1].Pitstop2(20-40μM;30min)doesnotaffecttheinternalizationofcholeratoxinBinJ774A.1macrophages1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].Pitstop2(0.001-100μM;6h)inhibitsthemitoticspindleandimpairsmitoticprogressioninHeLacells[2].Pitstop2(1-30μM;24h)inducesapoptosisandinhibitscellgrowthindividingcancercells[2].Pitstop2(1-30μM;48h)doesnotaffectthegrowthandviabilityofnon-tumourigenicNIH3T3fibroblasts[2].CellViabilityAssay[2]CellLine:HeLacellsConcentration:1,3,10,30μMIncubationTime:24hoursResult:ReducedthetotalnumberofviableHeLacellsmarkedlyinadose-dependentmanner.户使⽤本产品发表的科研⽂献•Immunity.2022Aug9;55(8):1370-1385.e8.•BioengTranslMed.26July2022.•Traffic.2022Nov22.•ResearchSquarePreprint.2021Oct.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].KumarGA,et,al.LeishmaniadonovaniInternalizesintoHostCellsviaCaveolin-mediatedEndocytosis.SciRep.2019Sep2;9(1):12636.[2].SmithCM,et,al.Inhibitionofclathrinbypitstop2activatesthespindleassemblycheckpointandinducescelldeathindividingHeLacancercells.MolCancer.2013Jan17;12:4.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.