简介Waters UPLC故障排除

简介WatersUPLC故障排除第1页/共53页WatersUPLCTroubleshootingTraining

第2页/共53页AgendaACQUITYUPLCFamily：UPLC&H-ClassWatersUPLCSystemUPLCSolventManagement

(BSM)UPLCSampleManagement

(SM)WatersACQUITY

H-ClassSystemQuaternarySolventManager(QSM)SampleManager-FlowThroughNeedle(SM-FTN)WatersUPLCDetectorTroubleshootingTrainingWatersUPLCPDAandPDAeλTUVFLRELSDOpenDiscussion

第3页/共53页ACQUITYUPLCFamily第4页/共53页ACQUITYUPLCSystem第5页/共53页ACQUITYUPLCSystemDetectors:Opticaland/orMassSpecTunableUVorPhotodiodeArrayOptimizedflowcellforUPLCHighspeeddatasamplingColumnManager:InnovativepivotdesignPositionscolumntodetectorE-CordconnectionSampleManager:LowdispersionXYZZ’FormatFastcycletimesLowcarryoverPlatesand/orvialsOptionalSampleOrganizerBinarySolventManager:HighpressureblendingBinarygradientsFoursolventchoicesOn-linedegassingLowdispersiondesignUPLCpressurecapabilitiesSampleOrganizer:

(option)Expandscapacity(22/15/8)ShuttlesplatefeedHeated/chilledSystemConsiderations:SmallFootprintRedesignedtubingandfittingsConsolidatedwastemanagementIntegratedsystemdiagnosticsConnectionsInsightremotediagnostics第6页/共53页A1A2B1B2WasteFluidics

andConnectionsTubingmanagementChromatographicsolvents:A1/A2,B1/B2TwoSampleManagerwashsolventsSealWashCommonfittingsandtoolsforusersColumninlets/outletsandfittingsFingertight,¼turn(use1/4”wrench)Dedicatedtubing3piececompressionscrewandferruleSmallfootprint–13½”wide24.25”withSampleOrganizer第7页/共53页UPLC

SolventManager第8页/共53页ACQUITYUPLCSystem

SolventDelivery

15KpsiperformanceAccurateandpreciseflowratesAccurateandprecisecompositionLowdelayvolumeforgradientapplications<120µLSystemVolumeLongtermreliability第9页/共53页BinarySolventManagerComponentsFilter/Mixer/TeeAssembly“A”PumpAccumulator“A”PumpSolventSelectValve“B”PumpSolventSelectValve“A”PumpPrimary“B”PumpPrimary“B”PumpAccumulatorSixChannelDegasser第10页/共53页IntelligentIntakeCheckValves第11页/共53页ACQUITYUPLCSystem

FromSerialtoParallelFlowTwoindependentlydrivenprimaryandaccumulatorpistondrivesSeparatedrivemotorsTwotransducersTwoinletcheckvalvesFourSolventsCapabilityA1orA2andB1orB2TwosolventspergradientSixChannelDegasser4chromatographiceluents2solventsforSampleManagerStrong&weakwash“B”“A”B2B1A2SolventA1第12页/共53页BinarySolventManager

InteractiveDisplay第13页/共53页RecommendedSolventsListACQUITYUPLCSystemrecommendedSolvents

Methanol Methanol/watermixtures Water Acetonitrile/watermixtures Acetonitrile(ACN) Isopropanol(IPA)Samplediluents(inadditiontothesolventslistedabove)

Dimethylsulfoxide(DMSO) Dimethylformamide(DMF)Additives/Modifiers

0.2%formicacid 0.1%trifluoroaceticacid(TFA) 0.1%triethylamine(TEA) 0.1%hexafluorobutericacid 10mMphosphatebuffer 10mMammoniumbicarbonate 50mMammoniumhydroxide 50mMammoniumacetate 0.1%Ethylenediaminetetraaceticacid(EDTA)Cleaners

Phosphoricacid(=30%) Sodiumhydroxide(=1M)ACQUITYUPLCSystemnon-recommendedSolvents

Methylene-Chloride Chloroform Strongacids>5% EthylAcetate Toluene Chlorinatedsolvents:(Trichlorobenzene)

第14页/共53页UPLC

SampleManager第15页/共53页SampleManagerFluidicsTray Pressure TransducerValveManifold

DriveGears

WashSyringesWeakWashStrongWashMeteringSyringeDriveAssembly第16页/共53页MeteringSyringeValve3WayRotaryValveMeteringSyringeAssemblySampleSyringe50uLvolumePN410001348100µLvolumePN700002570250µLvolumePN410001347forusewith50µLloop第17页/共53页ColumnHeatingCompartment第18页/共53页ACQUITYIn-LineFilter19第19页/共53页ColumnIn-LineFilterAssembly第20页/共53页ColumnIn-LineFilterAssembly第21页/共53页ColumnHeating第22页/共53页SampleManagerFlowPath第23页/共53页AvailableLoopsDescription P/N Status 1µLLoop 430001516 Optional2µLLoop 430001264 Optional5µLLoop 430001233 Optional10µLLoop 430001326 Standard20µLLoop 430001320 Optional50µLLoop 430001325 Optional100µLLoop 170001792 (Kit)Optional250µLLoop 176001780 (Kit)Optional第24页/共53页VolumeConfigurationMenuBeforerunningtheCharacterizeNeedleandLoopVolumefunction,makesurethepropervolumesareconfiguredintheVolumesConfigurationmenu.TheabovevaluesarestandardforcurrentlyshippingACQUITYsystem.第25页/共53页RecommendedInjectionVolumes,

LoopSizesandInjectionModesLoopVolume(uL)PartialLoopw/NeedleOverfillModeRecommendedsampleinjectionvolumerange(uL)PartialLoop(PressureAssistMode)Recommendedsampleinjectionvolumerange(uL)FullLoopModeRecommendedsampleinjectionvolume(uL0Automaticoverfillfactor1uL0.2to0.75NotRecommended1.05.82uL0.4to1.5NotRecommended2.05.65uL1.0to3.8NotRecommended5.0510uL2.0to7.52.0to5.010.0420uL4.0to15.04.0to10.020.0350uL10.0to37.510.0to25.050.02100uL20.0to75.020.0to50.0100.02250uL40.0to150.040.0to125.0250.01.5RecommendedPLNOinjectionvolume20%to75%ofloopvolumeRecommendedPartialLoopInjection20%to50%ofloopvolume第26页/共53页ACQUITYH-ClassSystemQSMQuaternarySolventManagerSM-FTNSampleManager-FlowThroughNeedleCH-AHighTemperatureColumnHeaterActivePre-HeatingDetectorsTUV,PDAePDA,FLR,ELSD第27页/共53页TheACQUITYH-ClassSystemconsistsofthefollowing:QuaternarySolventManager(QSM)FoursolventlowpressuregradientmixingSupportsUPLCPressuresLowgradientdelayvolumeSM-FTN(SampleManager-FlowThroughNeedle)Oneinjectionmode-directinjecttypeMuchsimpler-JustspecifyinjectionvolumeExcellentrecoveryandprecisionCH-AHightemperaturecolumnheaterwithactivepre-heatingACQUITYH-ClassSystem第28页/共53页QSMFluidics-FrontViewPrimaryHeadAccumulatorHeadVentValve100ulMixerGradientProportioningValvePairsLowPressureManifoldDegasserChambersSealWashPump第29页/共53页QSMConsoleTopLevelMenu第30页/共53页ACQUITYH-ClassSystem第31页/共53页第32页/共53页SMDIFrontPanelFluidicsMeteringSyringeValveCheckValveandUnionInjectValveWashSolventInletLine(notdegassed)LeakSensorConnectionsSampleMeteringSyringe第33页/共53页SDMIInjectValveConnectionsToCheckValveToCH-AandColumnStationarySideTubeFromPumpToPressureTransducerToSampleNeedle第34页/共53页SampleCompartmentPowerandStatusLEDsPlateSelectorSwitchRotaryTray(PlateHolder)MagneticDoorSensorPowerSwitch第35页/共53页SM-FTNInteractiveDisplayMenu第36页/共53页CH-AImprovedDoorGasketAddedTubingGasket(topandbottom)For3rdpartyMassSpecatleftsideofACQUITYStack.AllowsuseofexistingPassivePre-heaterNowirestoconnectActivePre-heater第37页/共53页InstallingtheActiv