兽医专业英语（扬州大学）l

LessonFifteenSalmonellaTesting

精选pptNewwordsandphrasesSalmonellaBacteriologicalImmunologicalEnzymicChromogenicImmunochromatographicListeriaFloraAgarPeptoneMagneticStreakLuminescentReagentGiardiaCryptosporidiumProtozoa(zoon)StreptavidinImmunosorbentBrothImmobilizeInoculation精选pptTextThefoodmarketcontinuestogrowwiththeintroductionofnewproductsandnovelmethodsofpresentation.Theincreaseinsamplenumbershasprovidedimpetus(推动力)forthedevelopmentofnewtestswhicharedesignedtostreamlinethetestmethodsandprovidereliableandeconomicalresultstoensureboththebacteriologicalsafetyandlong-termstabilityofthefoodproducts.精选pptImmunological,enzymicandmolecularbiologicaltechniquesarenowappearingastestmethodsinadditiontotheroutineagarplatedetectionmethods.Althoughprovidinggreatsensitivityandspecificity,manymethodsstillrequiresignificantcapitalinvestmentinequipmentandskilledpersonnelinordertoattainbesttestperformance.精选pptForSalmonellatestingtheabilitytoprovidequick,sensitiveandaccuratetestresultsisimportanttoensurethatthisdiverseandcommonlyencounteredgroupofpathogensisnotdistributedintothehumanfoodchain.精选pptForqualitycontrolofrawmaterialsthekeyproblemisoftenthedetectionoflownumbersofviablepathogensinthepresenceofmuchgreaternumbersofbackgroundflora.Thisisasignificantproblemifthefinalproductisnotrequiredtoreceivefurthercooking.Incaseswherefoodproductsarepartiallycooked,thenchilledpriortosale,theproblembecomesoneofdetectionrequiringatestmethodwithhighsensitivitytothetargetorganism.精选pptConventionaltestsrequireaperiodofnonselectiveenrichment

followedbyafurtherperiodofenrichmentinaselectivegrowthmedium,whereconditionsfavourgrowthofthetargetorganism,whichcansubsequentlybeculturedbyplatingontoaselectiveagarplate.精选pptThesearchforlownumbersoftargetorganismshasalsobeensignificantlyimprovedbytheuseofimmuno-linkedmagneticbeads,whichcanbeusedto“select”specifictargetorganismsfromamixedculture.Themagneticbeadsarelinkedwithanantibodywhichisspecificforthetargetorganism.Thesebeadsdonotpossessanynativemagnetismbutexhibitmagneticpropertiesonlywhenintroducedintoamagneticfield.精选pptNowtheimmuno-linkedmagneticbeadscanbeusedtocapturetargetorganisms,andthebeadscanthenbeseparatedfromthemixturebyapplicationofastrongmagneticfieldandtheunwantedmixturediscarded.Thebeadpreparationcanthenbewashedandusedforfurtheranalysis,either

bystreakingontoselectiveagarplates

or

bycombinationwithluminescentreagents

inordertorapidlydetectlowlevelsofthetargetorganism.精选pptMagneticbeadsareavailablefordetectionofSalmonella,andE.coliO157:H7.SimilarpreparationsarealsoavailablefordetectionofGiardiaandCryptosporidium,protozoawhichposesignificantproblemsforthewaterindustry.UseofStreptavidinlinkedbeadsprovidesanopportunitytocoatmagneticbeadswithspecificantibodiesofinterest,thusopeningthewayfornewmethoddevelopmentanddetectionoflesscommonmicroorganisms.精选pptAlternativeimmunosorbenttechnologyformsthebasisofaquickandsimpletestdesignedtoscreenenrichmentbrothsamplesforthepresenceofSalmonellaorganisms(antigen).SpecificantibodiesareimmobilizedonapermeablesupportandusedtocaptureSalmonellaantigenswhichmaybepresentinthetestsample.Allthenecessarytestreagentsarepresentinthetestdeviceandthetestcarriedoutsimplybyintroductionofafewdropsofthesampleenrichmentbrothintothetestwell.精选pptThesamplediffusesthroughthereactiontracktogiveatestandcontrolresult----visibleasacolouredlineintheviewingwindow.Testscanbecarriedoutonenrichmentbrothsamplesafteronly24hourswithavisibleresult10~15minutesafterinoculationofthedevice.

精选ppt鸡沙门氏菌的分离鉴定及药物敏感试验SeparationIdentificationandDrugSusceptibilityTestofAvianSalmonella摘要：从新疆南疆地区某鸡场采集到的14份具有沙门氏菌病病变特征的病料中分离出沙门氏菌10株.Abstract:14sampleswithaviansalmonellosiswerecollectedfromsouthernareaofXinjiang，and10strainsofchickenSalmonellawereseparated．标题精选ppt通过形态观察、生化试验、血清学鉴定及动物致病性试验，结果表明，10株沙门氏菌的血清型为O2、O9，这两种血清型沙门氏菌对5日龄雏鸡具有很强的致病作用.Theresultsofmorphologicalobservation，biochemicaltests，serotypeidentificationandpathogenicitytestsshowedthatO2andO9werethedominantserogroupsandbothserogroupswerehighpathogenicforchickens5daysold．精选ppt药敏试验结果表明，沙门氏菌对硫酸庆大霉素最敏感，其次是头孢唑啉钠，敏感率分别为100％和90％，其对乙酰螺旋霉素和罗红霉素产生耐药性.Thedrugsusceptibilitytestindicatedthatthesensitivitiesofthe10strainstogentamycinsulfateandcefazolinsodiumwere100％and90％，respectively．Andtheywerehighresistancetoacetylspiramycinandroxithromycin．关键词：鸡；沙门氏菌；分离；鉴定；药物敏感试验Keywords:chicken；Salmonella；separation；identification；drugsusceptibilitytest精选pptRapidHygieneTestingTechnologyRapidhygienetestingtechnologies,suchasthoseofthemarketleaderBiotrace,aremostlybasedonthemeasurementofadenosinetriphosphate(ATP).ATPischemicalfoundinalivingcells.So,ifATPispresentonasurfacethatappearstobecleantothehumaneye,somefoodresiduesorothercellularmaterialmustbepresentcreatingapotentialbreedinggroundforbacteria.精选pptHarnessingthisprinciple,theATPb