遗传学翻转课堂：实验设计

Anti-VEGF/VEGFR2axisenhancesradio-resistanceviaregulatoryT-celldepletion

2016-11-31.RadiationenhancesregulatoryTcellrepresentation2.Tregcontributetotheresistanceoftumortoradiation3.VEGFA-VEGFRpathwayblockadecaninhibitregulatoryT-cellproliferation4.RaisingmyhypothesisContents1.RadiationenhancesregulatoryTcellrepresentationHerreraFG,etal.CACancerJClin.2016Aug29.ImmuneMechanismsTriggeredbyTumorIrradiationHerreraFG,etal.CACancerJClin.2016Aug29.RadiotherapyReprogramstheTumorMicroenvironmentReportedClinicalCasesofAbscopalEffectsofRadiotherapyinCombinationWithImmunotherapyHerreraFG,etal.CACancerJClin.2016Aug29.Tregcellsincreasetemporarilyafterlocalirradiation.KachikwuEL,etal.IntJRadiatOncolBiolPhys.2011Nov15;81(4):1128-35.Theradiation-inducedincreaseinTregcellsisdosedependent.KachikwuEL,etal.IntJRadiatOncolBiolPhys.2011Nov15;81(4):1128-35.Lowradiationdosestothewholebodyreducedspleensize,weight,andcellnumberswhileincreasingtheproportionofsplenicTregulatory(Treg)cells.KachikwuEL,etal.IntJRadiatOncolBiolPhys.2011Nov15;81(4):1128-35.DifferentresponsesofCD4+CD25highTcellsandCD4+CD25-Tcellstoirradiationinvitro.QuY,etal.RadiatRes.2010;173:148-157.FlowcytometryanalysisofBcl-2expressioninCD4+CD25highTcellsandCD4+CD25-Tcells.QuY,etal.RadiatRes.2010;173:148-157.ShimizuJ,etal.NatImmunol.2002Feb;3(2):135-42.PriceJG,etal.NatImmunol.2015Oct;16(10):1060-8.LiuS,etal.AmJCancerRes.2015Oct15;5(11):3276-85.2.TregcontributetotheresistanceoftumortoradiationSchematicoftheprotocolforcombinationtreatmentofradiationandlow-doseCTXoranti-CD25mAbSonCH,etal.IntJRadiatOncolBiolPhys.2015Jun1;92(2):390-8.Low-doseCTXoranti-CD25mAbtreatmentsignificantlyenhancesthetherapeuticefficacyofradiationtherapySonCH,etal.IntJRadiatOncolBiolPhys.2015Jun1;92(2):390-8.Low-doseCTXoranti-CD25mAbtreatmentcombinedwithradiationtherapysignificantlyprolongssurvivaltime.SonCH,etal.IntJRadiatOncolBiolPhys.2015Jun1;92(2):390-8.Tregsinterferewithirradiation-inducedk1106cellapoptosis.LiQ,etal.CellMolImmunol.2015;12:366-72.LiuS,etal.AmJCancerRes.2015Oct15;5(11):3276-85.3.VEGFA-VEGFRpathwayblockadecaninhibitregulatoryT-cellproliferationAnti-VEGF-AtreatmentssignificantlyreduceTregproportionsandnumbersTermeM,etal.CancerRes.2013Jan15;73(2):539-49.TermeM,etal.CancerRes.2013Jan15;73(2):539-49.SunitinibtreatmentssignificantlyreduceTregproportionsandnumbersTermeM,etal.CancerRes.2013Jan15;73(2):539-49.SunitinibtreatmentssignificantlyreduceTregproportionsandnumbersTregsproliferateinresponsetoVEGF-Astimulation.TermeM,etal.CancerRes.2013Jan15;73(2):539-49.Tregsintumor-bearingmiceexpressVEGFreceptorsTermeM,etal.CancerRes.2013Jan15;73(2):539-49.Tregsintumor-bearingmiceexpressVEGFreceptorsandtheirproliferationisVEGFR-2–dependent.TermeM,etal.CancerRes.2013Jan15;73(2):539-49.VoronT,etal.FrontOncol.2014Apr2;4:70.4.RaisingmyhypothesisTianS,etal.CancerSci.2011;102:1374–80.Anti-VEGF/VEGFR2axisenhancesRadio-resistanceviaregulatoryT-celldepletion1体内研究照射后及外周血、脾脏、引流淋巴结Treg绝对含量及百分比

2检测荷瘤小鼠Treg表达VEGFR2情况

3加阿帕替尼后检测外周血、脾脏、引流淋巴结Treg绝对含量及百分比

4体外研究阿帕替尼联合放疗对Treg功能的影响

5放疗和阿帕替尼联合方式：“时间窗”，续贯或同步