Crystal-Violet-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECrystalViolet(solution)Cat.No.:HY-DY2001CASNo.:548-62-9分⼦式:C₂₅H₃₀ClN₃分⼦量:407.99作⽤靶点:FluorescentDye;InfluenzaVirus;Bacterial作⽤通路:Others;Anti-infection储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性CrystalViolet(solution)，也称为龙胆紫、甲紫10B，⼀种三苯甲烷类染料，碱性染料，可以和细胞核中的DNA结合，从⽽将细胞核染为深紫⾊。常⽤于⾰兰⽒染⾊来进⾏细分类，或者⽤于细胞或组织学染⾊[1]。溶剂及浓度：⽆⽔：5mg/mL(0.5%)体外研究GeneralProtocol1.PreparationofMitoSOXRedworkingsolutionDilutethe2gCrystalVioletin20mL95%ethanolandmixedwith80ml1%ammoniumoxalatesolutionsolutionfor24htoobtainworkingsolution.2.Sampleprocessing2.1Paraffinsection:Firstdewaxingwithxylenefor5-10min,thenwithfreshxylene,thendewaxingwith100%ethanolfor5minafterdewaxingfor5-10min.Then90%ethanolwasusedfor2min.Atlast,70%ethanolwasusedfor2min.DDH2O2min.2.2Frozensection:distilledwaterfor2min.2.3Tissueculturecells:fixedwith4%paraformaldehydeformorethan10min.Washwithdistilledwaterfor2min,replacewithfreshdistilledwater,andwashagainfor2min.Note:Otherfixativescanbeselectedaccordingtospecificexperiments.3.Crystalvioletstaining1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE3.1Afterthesampletobetestedistreatedwiththeabovemethod,thecrystalvioletdyeingsolutionisdirectlyaddedanddyedfor10minutesatroomtemperature(thedyeingsolutionneedstocoverthesample,andthespecificdyeingtimecanbeadjustedaccordingtotherequireddyeingresults).3.2Washwellindistilledwater.3.3Dryatroomtemperature.3.4Observeandphotographbylightmicroscope.Precautions1.Itisrecommendedtotake1-2samplesforpre-testwhenusingthisproductforthefirsttime.2.ThisproductisforR&Duseonly,notfordrug,household,orotheruses.3.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].NagayamaA.InactivationofinfluenzaAvirusbygentianviolet(GV)andGV-dyedcottoncloth,andbactericidalactivitiesoftheseagents.JInfectChemother.2006Apr;12(2):73-9.[2].Beveridge,T.J.andS.Schultze-Lam,Theresponseofselectedmembersofthearchaeatothegramstain.Microbiology,1996.142(Pt10):p.2887-95.[3].Coico,R.,Gramstaining.CurrProtocMicrobiol,2005.Appendix3:p.Appendix3C.[4].Bil,J.,etal.,Statinspotentiatecytostatic/cytotoxicactivityofsorafenibbutnotsunitinibagainsttumorcelllinesinvitro.CancerLett,2010.288(1):p.57-67.[5].XiaoX,etal.Analysisoftracemalachitegreen,crystalviolet,andtheirmetabolitesinzebrafishbysurface-coatedprobenanoelectrosprayionizationmassspectrometry.Talanta.2020Sep1;217:121064.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedical