dysadherin在肿瘤中的表达及其意义

? dysadherin ? 2002Ino1Ecadherin dysadherindysadherin dysadherin ,dysadherindysadher indysadher in ? 1? dysadherin ? dysadherincDNA178O 12dysadherin 312 dysadherinT dysadherin cDNAPLC/PRF/5dysadherin dysadherin1 ? 2? dysadherin ? dysadherinactin organizationEcadherin dysadherinCCL2 dysadherin ? 2.1? dysadherin ? dysadherin 13 ? Shimamura3dysadherin dysadherin(siRNAsmall interfering RNA)Panc1dysadherin dysadherin dysadherin dysadherinCapan1 6dysadherin dysadherindysadherin dysadherin cDNAsiRNAdysadherin dysadherinSCID dysadherinCapan1SCID35 dCapan1mocktransfectan teddysadherinCapan1 ? dysadherin ? 2.2? dysadherinEcadherin ? dysadherinEcadherin Ino 1dysadherindysadherin cDNAPLC/PRF/5mRNAdysadherin Western blotdysadherin cDNAdysadherin cDNAEcadherinNorthern blotsdysadherin cDNAEcadherin dysadherinEcadherin Ecadherin mRNA611 dysadherinEcadherin dysadherinEcadherin1 ? ,Ino 1 dysadherin cDNAPLC/PRF/5 E cadherin 13dysadherinEcadherin Ecadherin1 ? dysadherinOEcadherinTsui ji2dysadherinOO dysadherin EcadherindysadherinO benzylGalNAc dysadherindysadherinO dysadherinEcadherinO EcadherindysadherinOEc adherin ? 459 1011 dysadherinEcadherin 3dysadherin EcadherinEcadherin dysadherinEcadherin dysadherin ? 2.3? dysadherin ? dysadherinCCL2Nam 14Ecadherindysadherin Ecadherin, Ecadherindysadherin dysadherinMDAM B231dysadherinCCL2 dysadherinNFB(nuclear factorB pathway)CCL2dysadherinCCL2 CCL2MDAMB231 ,dysadherinCCL2 the prometastatic effect ? 3? dysadherin3.1? ? dysadherin Shimamura4dysadherinmAb NCCM53125 dysadherin dysadherin dysadherin dysadherin125 0 20%2321% 50%3851% 100%640 20%dysadherin41.621% 50%22.251% 100%11.30 20%dysadherin7/23511 dysadherin0 20%dysadherin21% 50%51% 100%dysadherin Ecadheri n50% 100% dysadherinEcadherin9.1 0 50%dysadherinEcadherin 31.7dysadherin Ecadherin ? 3.2? ? dysadherin Shimada5276dysadherin dysadherin dys adherin EcadherindysadherinEcadherin dysadherinEcadherin dysadherin 3.3? ? Sato692511031 dysadherindysadherin 10dysad herindysadherin dysadherin5112dysadherin 2874 dysadherin12193 dysadherin,dysadherin dysadherinpTNMpT4dysa dherinpT3pT2pT1 (P0.001)dysadherindysadherinpN1 pN0P0.001M1dysadherinM0 P0.011dysadherin dysadheri n ? 3.4? ? Wu7206dysadherin dysadherin dysadherindysadher indysadherin dysadherinP 0.04FIGOdysadhe rin ? 3.5? ? dysadherinAoki88 2dysadherin 51%50%dysadherindysadheri nP0.003 P0.0099P0.0036dysadherin EcadherinP0.0043 ,P0.0028dysadherin ? 3.6? ? 91011 dysadherindysadherin ? 4? ? ? dysadherin dysadherin dysadherin dysadherin dysadherin ? ? 1INO Y,GOTOH M,SAKAMOTO M,et al.dysadherin,a cancerassociated cell membrane glycoprotein,downregulates Ecadherin and promotes metastasisJ.Proc Natl Acad Sci U S A,2002,99(1):365370. 2TSUIJI H,TAKASAKI S,SAKAMOTO M,et al.Aberrant Oglycosylation inhibits stable expression of dysadherin,a carcinomaassociated antigen,and facilitates cell adhesionJ.Glycobiology,2003,13(7):521527. 3SHIMAMURA T,YASUDA J,INO Y,et al.dysadherin expression facilitates cell motility and metastatic potential of human pancreatic cancer cellsJ.Cancer Res,2004,64(19):69896995. 4SHIMAMURA T,SAKAMOTO M,INO Y,et al.dysadherin overexpression in pancreatic ductal adenocarcinoma reflects tumor aggressiveness: relationship to Ecadherin expressionJ.J Clin Oncol,2003,21(4):659667. 5SHIMADA Y,YAMASAKI S,HASHIMOTO Y,et al.Clinical significance of dysadherin expression in gastric cancer patientsJ.Clin Cancer Res,2004,10(8):28182823. 6SATO H,INO Y,MIURA A,et al.dysadherin: expression and clinical significance in thyroid carcinomaJ.J Clin Endocrinol Metab,2003,88(9):44074412. 7WU D,QIAO Y,KRISTENSEN G B,et al.Prognostic significance of dysadherin expression in cervical squamous cell carcinomaJ.Pathol Oncol Res,2004,10(4):212218. 8AOKI S,SHIMAMURA T,SHIBATA T,et al.Prognostic significance of dysadherin expression in advanced colorectal carcinomaJ.Br J Cancer,2003,88(5):726732. 9TAMURA M,OHTA Y,TSUNEZUKA Y,et al.Prognostic significance of dysadherin expression in patients with non small cell lung cancerJ.J Thorac Cardiovasc Surg,2005,130(3):740745. 10NISHIZAWA A,NAKANISHI Y,YOSHIMURA K,et al.Clinicopathologic significance of dysadherin expression in cutaneous malignant melanoma: immunohistochemical analysis of 115 patientsJ.Cancer,2005,103(8):16931700. 11NAKANISHI Y,AKIMOTO S,SATA Y,et al.Prognostic significance of dysadherin expression in tongue cancer: immunohistochemical analysis of 91 casesJ.Appl Immunohistochem Mol Morphol,2004,12(4):323328. 12KYZAS P A,STEFANOU D,BATISTATOU A,et al.dysadherin expression in head and neck squamous cell carcinoma: association with lymphangiogenesis and prognostic significanceJ.Am J Surg Pathol,2006,30(2):185193. 13RIMM D L,KOSLOV E R,KEBRIAEI P,et al.1(E)catenin is an actinbinding and bundling protein mediating the attachment of Factin to the