课件：高美华《医学免疫学》..ppt

Experiment 2,Precipitation,Definition: Soluble Ag + Ab Ag-Ab complex Classification: Precipitation reaction in solution Precipitation reaction in gels Immunoelectrophoresis Technique Immunoturbidimetry,Precipitation Reactions In Gels,Agar、Agarose Precipitation reactions between soluble antigens and antibodies can take place in semisolid media such as agar gels. When soluble antigen and antibodies are placed in wells cut in the gel, the reactants diffuse in the gel and form gradients of concentration, with the highest concentrations closest to the wells. Some where between the two wells, the reacting antigen and antibodies will be present at proportions that are optimal for formation of a precipitate.,Double Agar Diffusion Test,analysis of the properties of Ag Patterns of Identity Patterns of Nonidentity Patterns of Partial Identity: spur,single agar diffusion test,The wells contain antigen at different concentrations, while the antibodies are distributed uniformly in the agar gel. Thus, the precipitin line is replaced by a precipitin ring around the well. The distance the precipitin ring migrates from the center of the antigen well is directly proportional to the concentration of antigen in the well.,Method,Result,Counter Immunoelectrophoresis,pH88.6 buffer,Ag： PI =5 MW：small Electric field force :cathodeanode，strong Electroosmosis: anodecathode Electric field force Electroosmosis Direction of phoresis: cathodeanode,Ab： PI =7 MW：large Electric field force :cathodeanode，weak Electroosmosis: anodecathode Electric field force Electroosmosis anodecathode, Cathode,+Anode,Ag,Ab,Result,Rocket Immunoelectrophoresis,Immunoelectrophoresis,Immunoelectrophoresis involves separating a mixture of proteins in an electrical field (electrophoresis) followed by their detection with antibodies diffusing into the gel.,a small drop of human serum is placed in a well cut in the center of a slide that is coated with agar gel. The serum is then subjected to electrophoresis, which separates the various components according to their mobilities in the electrical field. After electrophoresis, a trough is cut along the side of the slides, and antibodies to human serum proteins are placed in the trough.,The antibodies diffuse in the agar, as do the separated serum proteins. At an optimal antigen:antibody ratio for each antigen and its corresponding antibodies, they form precipitin lines. Comparison of the pattern and intensity of lines of normal human serum with the patterns and intensity of lines obtained with sera of patients may reveal an absence, overabundance, or other abnormality of one or more serum proteins.,后面内容直接删除就行 资料可以编辑修改使用 资料可以编辑修改使用 资料仅供参考，实际情况实际分析,主要经营：课件设计，文档制作，网络软件设计、图文设计制作、发布广告等 秉着以优质的服务对待每一位客户，做到让客户满意！ 致力于数据挖掘，合同简历、论文写作、PPT设计、计划书、策划案、学习课件、各类模板等方方面面，打造全网