膜泡运输的结构基础PPT课件.ppt

Figure13-3MolecularBiologyoftheCell(GarlandScience2008),IntracellularVesicularTraffic,1,Locationofmicrotubule-associatedproteins,2,Majormicrotubule-associatedproteins,3,ThecellstransfectedwithtautypeL4,4,TheimportanceofMAPsforneuriteformation,5,Cross-sectionelectronmicrographsofMAP-inducedprocessesinSf9cells,6,MAP2,MAP2C,tau,MAP2invitro,Tauinvitor,7,OrganizationofMTbundlesbyMAPs.SpacingofMTsdependsonMAPs,InsectcellexpressingMAP2,Insectcellexpressingtau,J.Chenetal.Nature.1992,8,Cytoskeletalarchitecture,AXON,DENDRITE,9,KinesinandDynein,10,Microtubule-basedtransportandintracellulartraffickingpathways,Hirokawaetal.,2009,11,12,Haradaetal.,JCB,1998,Golgivesiculationandlysosomedispersionincellslackingcytoplasmicdynein,13,Molecularmotor-kinesin,14,StructuresofN-kinesins,M-kinesinsandC-kinesins,15,PhylogenicanalysisofallKIFsexpressedinmouse/humans,D.melanogaster,C.elegans,andS.cerevisiae.,16,17,Thestructureandphylogenyofmajormousekinesins,Hirokawaetal.,2009,18,QWangetal.,CellRes.2010,19,PrincipalmembersofKIFsobservedbylowanglerotaryshadowing,Hirokawaetal.,2005,20,KIFsbindtocargoesthroughadaptororscaffoldingproteincomplexes,Hirokawaetal.,2005,21,22,23,24,25,Tanakaetal.,Cell.93:1174-1158.1998,26,27,KIF3isfunctionallydiversefamilyofmotor,Left/rightdeterminationRetinitispigmentosaPolycystickidneydisease(PKD),Tumoursuppressoradenomatouspolyposiscoli(APC)FodrinSmallGTP-bindingproteindissociationfactorSmgGDSPAR-3,NonakaS.etal.,1998Cell,95:829837,28,29,30,31,32,Kartagener综合征又称支气管扩张-鼻旁窦炎-内脏转位综合征,33,TheKIF3MotorTransportsN-cadherinandOrganizestheDevelopingNeuroepithelium,34,Hypertrophyandinvadingtumor-likerosettephenotypeofkap3cKOmouse,35,KAP3-deficientleadstomalignanttransformationofneuroepithelium,36,N-cadherinandbeta-cateninlevels