RANDOX质控血清使用说明.doc

RANDOX质控血清使用说明使用前准备1 小心打开瓶盖，避免内容物的损失2 每一瓶用5ml重蒸水复溶3 轻轻晃动，使干粉充分溶解4 大约30分钟后，即可使用5 注意不要剧烈摇动瓶子稳定性复溶后常温（25）可稳定24小时；2-8可保存一周。-20冰冻保存可达一个月以上注意：不要反复冻融注意事项1 ALP的水平当超过稳定期时会升高，建议复融后1小时内进行检测（室温条件下）。2 血清中胆红素对光敏感，注意避光保存。3 总ACP、前列腺ACP和醛缩酶项目稳定性可通过下面的措施得到提高：在每血清中加入一滴（25-30l）0.7M的醋酸溶液，室温下稳定2小时，2-8保存2天，冰冻条件下可保存一个月以上。4 该质控血清不可做校正品用。备注原文说明书中列有各类生化分析仪的平均值（MEAN OF ALL INSTRUMENTS）和10余种仪器的详细数值，请根据您的仪器对照查询。以下是各项目方法学的中英文对照，具体数值请依据使用试剂盒的方法原理在原文说明书中查询。项目方法METHODAlbumin溴钾酚绿Bromocresol Green溴钾酚紫Bromocresol Purple干化学 Dry ChemistryBicaebonate(co2)比色法Colorimetric滴定法Differential Rate pH change酶法Enzymetic电极法ISE干化学Dry ChemistryBile Acids酶比色法EnzymaticColorimeticDirect Bilirubin重氮法Diazo with Sulphanilic Acid干化学Dry ChemistryTotal BilirubinDCA法(二氯苯胺为底物)Diazo with Dichloroaniline(DCA)DPD法(重氮基二氯苯为底物)Dichlotophenyl Diazonium(DPD) 重氮法( DMSO 为催速剂)Diazo with Sulphanilic Acid干化学Dry ChemistryCalcium原子吸收Atomic Absorption偶氢砷法Arsenazo OCPC法Cresolphthalein complexoneMTB(甲基百里酚兰)Methylthymol blue干化学Dry ChemistryChloricle电量法Coulometry比色法Colorimetric离子间接电极法ISE Indirect离子直接电极法ISE DirectCholesterol同位素光谱测定Isotope Dilution-Mass Spectrometry氧化酶法 CHOD-PAPCholesterol Oxidase干化学Dry ChemistryCopper原子吸收Atomic Absorption比色法ColorimetricCreatinine同位素光谱测定Isotope Dilution-Mass Spectrometry苦味酸法(去蛋白)Alkaline picrate with deproteinization苦味酸法(不去蛋白)Alkaline picrate without deproteinizationTrinder 酶法Creatinine PAP methodRANDOX 酶法 (340nm)Enzymatic UV干化学Dry ChemistryGlucose同位素光谱测定Isotope Dilution-Mass SpectrometryGOD/O2-BeckmanGOD/O2-Beckman己糖激酶法Hexokinase氧化电极法Oxygen electrodeGOD PAP法Glucose oxidaseD-3-Hydroxybutyrate (D-3-羟丁酸)RANDOX试剂Tris buffer 100mM pH8.5Iron (铁)亚铁嗪法(需沉淀)Colorimetric with precipitation(Ferrozine Method)亚铁嗪法(不需沉淀)Colorimetric without precipitation(Ferrozine Method)TIBC去除过量的游离铁法Removal of Excess Free IronFe +未饱和铁Fe+UIBC(Saturation with Iron)Lactate(乳酸)酶比色法Enzymatic ColorimetricLithium(锂)火焰光度法Flame Photometry原子吸收法 Atomic Absorption离子电极法ISEMagnesiumCalmgite为染料Calmagite二甲苯胺蓝为染料Xylidyl BlueNEFA(非脂类脂肪酸)比色法ColorimetricOsmolaity(渗克分子浓度)计算法Calculated凝固点降压法Freezing Point depression蒸气压法Vapour PressurePhosphorus磷钼酸酶法(690nm)Phosphomolybdate enzymatic磷钼酸还原法(340nm)Phosphomolybdate readuction UVPhospholipids(磷脂)酶比色法Enzymatic Colorimetric钼酸盐-钒酸盐反应Molybdate/Vanadate ReactionPotassium(钾)酶法Enzymatic离子直接电极法ISE Direct离子间接电极法ISE IndirectPSA(前列腺特异抗原)亚培AxsymAbbott Axsym梅里埃DPCBiomerieux Vidas DPC ImmuliteSodium(钠)酶法Enzymatic离子直接电极法ISE Direct离子间接电极法ISE IndirectTotal Lipids(总脂类)酶比色法(硫酸磷酸香草醛)Enzymatic SulfophosphovanillinTotal Protein双缩脲反应(终点法)Biuret reaction,endpoint双缩脲反应(动力法)Biuret reaction,kinetic贝克曼 CX/4/5/7系列Biuret reaction CX4/5/7TriglyceridesGPO-PAP法(去游离TG)Lipase/GPO-PAP Color without Glycerol CorrectionGPO-PAP法(不去游离TG)Lipase/GPO-PAP Color with Glycerol Correction甘油激酶法UV(去游离TG)Lipase/Glycerol Kinase UV Endpoint without Glycerol Correction甘油激酶法UV(不去游离TG)Lipase/Glycerol Kinase UV Endpoint with Glycerol Correction甘油脱氢酶法Lipase/Glycerol DehydrogenaseUrea电导法Astra-conductivityO-邻苯二甲脱水酶法O-Phthalaldehyde脲酶终点法Urease,Endpoint脲酶动力法Urease kenetic波氏比色法Urease Berthelot脲酶次氯酸盐法Urease HypochloriteUric Acid尿酸酶紫外法(340nm)Uricase,Catalase 340nm磷钨酸还原法Reduction Methods氧化酶比色法(加抗坏血酸)Uricase peroxidase,with Ascorbate 氧化酶比色法(不加抗坏血酸)Uricase peroxidase without AscorbateRandox(546nm)/BMRandox 546nm / BM Uric Acid plusZinc比色法ColorimeticAcid Phosphatase (总ACP)以1-磷酸萘酚为底物的动力法1-Naphthyl Phosphate,Kinetic以1-磷酸萘酚为底物的动力法(以戊二醇为激活剂)1-Naphthyl Phosphate,Kinetic with Pentane Diol Activation对硝基磷酸做为底物p-NitrophenylphosphateAcid Phosphatase/Prostatic(前列腺酸性磷酸酶)以1-磷酸苯酚为底物的动力法1-Naphthyl Phosphate,Kinetic以1-磷酸苯酚为底物的动力法(以戊二醇为激活剂)1-Naphthyl Phosphate,Kinetic with Pentane Diol Activation对硝基磷酸做为底物p-NitrophenylphosphateAldolase(醛缩酶)紫外法(20min)UV Test 20minALPIFCC法(AMP为缓冲液)p-Nitropherrylphosphate,AMPDGKC法(DEA为缓冲液)p-Nitropherrylphosphate,DEAALT/GPTDGKC法DGKCSCE法(常用于双试剂)SCEIFCC法(底物中加入磷酸吡哆醛)IFCC withPyridoxal-5-phosphateIFCC法(底物中不加入磷酸吡哆醛)IFCC NO Pyridoxal-5-phosphateAmylaseRANDOX试剂(苯甲基阻断)Randox Benzylidene BlockedRANDOX亚甲基阻断PNP-G7法Randox Ethylidene BlockedBM和RANDOX”71”系列的EPS法Boehringer EPS and Randox “71” SeriesBM和RANDOX的EPS法(液体试剂)Randox EPS Liquid/BM EPS Liquid拜耳(Bayer)试剂Bayer/Technicon blocked Maltoheptaoside生物-梅里埃试剂Biomerieux blocked MaltoheptaosideSigma试剂Sigma blocked Maltoheptaoside长征试剂Trace blocked Maltoheptaoside贝克曼系列Beckman Synchron CX4/5/7 blocked Maltotetraose SubstratesPancreatic Amylase(胰腺淀粉酶)BM和RANDOX的EPS法Boehringer EPS and Randox “71” SeriesAST/GOT同ALT/GPTSame with ALT/GPTCholinesterase(CHE)丁酰硫代胆碱为底物Colorimetic Butyrylthiocholine乙酰硫代胆碱为底物Colorimetic AcetyrylthiocholineCK(CPK)DGKC法(单试剂)DGKC,serum startDGKC法(双试剂)DGKC,substrate start-GT以-谷氨酰-3-羧基-硝基苯胺为底物Gamma Glutamyl-3-Carboxy-4-nitroanilide以-谷氨酰-P-硝基苯胺为底物Gamma Glutamyl- 4-nitroanilideGLDHDGKC法DGKC-HBDHDGKC法DGKCLAP速率法(405nm)NAGELLDHP法:DGKCPL,DGKCP法:SCE(双试剂)PL,SCEP法:SFBCPL,SFBCL法:常用方法LPLipase(脂肪酶)浊度法Turbidimetric with Colipase 项目名称方法METHODCortisol皮质醇放射免疫法RIADigoxin地高辛免疫荧光法Rolarisation FluoroimmunoassayFolate叶酸同位素蛋白结合检测Competitive Protein Binding AssayGentamycin庆大霉素免疫荧光法Rolarisation FluoroimmunoassayParacetamol对乙酰氨基酚免疫荧光法Rolarisation FluoroimmunoassaySalicylate水扬酸盐TrinderColorimetric TrinderTheophylline茶碱免疫荧光法Rolarisation FluoroimmunoassayTobramycin吐霉素免疫荧光法Rolarisation FluoroimmunoassayApolipoprotein A-1载脂蛋白A1免疫比浊法ImmunoturbidimetricApolipoprotein B载脂蛋白B免疫荧光法ImmunoturbidimetricIgALgA免疫荧光法Imm