大鼠脑挫伤后HIF1α蛋白表达的法医学研究

大鼠脑挫伤后HIF1蛋白表达的法医学研究 【摘要】目的探讨大鼠脑挫伤后hifla蛋白表达的规律。方法参照feeney，s法建立大鼠脑挫伤模型，分 别于伤后2h，6h，12h，24h，2d，3d，5d，7d，14d取脑，运用免疫组化sabc法观察hifla蛋白的表达。 结果对照组偶见 阳性反应细胞。损伤后2h，即可观察到hifla蛋白表达。6h后表达明显增强，24h达高峰，随后阳性反应细胞逐渐减 少，7d后仍可见少量表达，14d后恢复。表达呈弥漫性，以大脑皮层、海马、脑干最为明显，不同部位的变化趋势一致。 各实验组与对照组比较有统计学意义(p 间的推断。 【关键词】脑挫伤；法医学；hifla；免疫组织化学；损伤经过时间 【】d9191 【】a 【】10079297(20xx)o1007603 study on the expression of hifla protein after cerebral contusion in rats and its forensic impficafionk伽g dengfeng,liu yanjun，wang yingyuanschool offorensic ndicine，shanxi medical university，taiyuan 030001，china 【abstract】objective to explore the expression of hifla protein in diferent time after cerebral contusion() methods based on feeneys model of and took the samples at 2h，6h，12h，24h，2d，3d，5d，7d，14d after brain injurythe expression of hifla protein was observed by immunohistochemical sabc and image analysis techniques results the expression of hif-la protein could be detected at 2h after injury，increased at 6h and peaked at 24hpositive cells de creased with the prolongation of injury timelittle expression was found at 7d the control group and the 14d group showed negative stainingthe expression of hifla protein was found in wide range but significant in cortex，hippocampus and brain stemthe changing tendency of diferent areas was sameconclusion expression of hifla protein is corre lated with post-traumatic intervals after in rats， suggesting that the expression of hif-let may be served as the mark ers for timing of in forensic practice 【key words】cerebral contusion，forensic medicine，hypoxia inducible factor-let，immunohistochemistry，brain in jurytime 低氧诱导因子(hypoxia inducible factor一1，hif一 1)是一种随着细胞内氧浓度变化而调节基因表达的 转录激活因子。缺氧时它能诱导多种缺氧应激蛋白 基因的表达，从而介导各种缺氧适应性反应。它被证 实与神经细胞在缺氧状态下的生