毒死蜱降的微生物降解及中间产物分析 论文正文

A1A0A2A3A4A5A6A7A9A10A8A12工程大学本科毕业设计（论文）专业生物工程题目毒死蜱的微生物降解及中间产物分析作者姓名导师及职称（教授）导师所在单位工程大学二零一零年五月二十六日A1A0A2A3A4A5A6A7A9A10A8A12A13毒死蜱降解菌株的降解谱及中间产物分析摘要随着世界范围内农药的广泛使用尤其是毒死蜱类有机磷农药的应用，使得农药污染成为环境污染的一个重大问题。本课题（毒死蜱降解菌株的降解谱及中间产物分析）有利于解决农药污染问题，为其提供新的思路和方法。研究毒死蜱降解菌株的降解谱及中间产物的分析在微生物农药降解领域有重要的研究和实践意义，中间产物的分析更是有利于深刻认识农药降解的整个过程。本论文实验中知，该实验中的毒死蜱降解菌株对毒死蜱降解能力较强,对50MG/L的毒死蜱最高降解率达7397,可为构建基因工程菌提供菌种资源,具有良好的研究价G1552和应用G2081G7235。在G17902过对G83G43G1552G451G5225物G8999G5242G12573环境因G4388G17839G15904G2345因G13044G3822G8712G5191实验,得G1998该降解菌株在30G263G451G83G4375G451G5225物G8999G524250MG的G7477G1226G991具有最G1351降解G5627能。该降解菌株G994G5062G6265G17959的毒死蜱生物降解的机G10714G11468G2528，G18129是G4570毒死蜱降解为G55G38G51，G1000G55G38G51G18129有G993G2528程G5242的G12227G13059。对于毒死蜱的环境污染G8847G10714，可G1209在毒死蜱污染G2318域G9163G2524G3533G1871毒死蜱降解菌和G55G38G51降解菌，G1209G7411提高环境污染的G8847G10714G6940率和G8847G10714G6940G7536，G4570毒死蜱G4448G1852降解为对环境G451生物G18129G7092污染G451G7092G11784G3363G451G7092毒的G2282G2524物。G1075可G1209G17902过G16837G2476G12591G17885G1998能G3827高G6940G451广谱降解毒死蜱G12573农药的G2476G5334降解菌株，G1209提高降解G6940G7536。关键词毒死蜱G727降解G727中间产物A15A11A14A16A17A18A19A20A21A22A23A24A25A19A26A27A13A13ABSTRACTWITHTHEWIDESPREADUSEOFPESTICIDESAROUNDTHEWORLD,ESPECIALLYORGANOPHOSPHORUSPESTICIDECHLORPYRIFOSAPPLICATIONCLASS，ITMAKEPESTICIDEPOLLUTIONISAMAJORPROBLEMOFENVIRONMENTALPOLLUTIONTHISISSUEHELPTORESOLVETHEPESTICIDEPOLLUTIONOFCHLORPYRIFOSDEGRADINGBACTERIAANDDEGRADATIONOFSPECTRALANALYSISOFINTERMEDIATEPRODUCTS,ITPROVIDENEWIDEASANDMETHODSFORTHEPESTICIDEDEGRADATIONTHEDEGRADATIONOFCHLORPYRIFOSDEGRADINGBACTERIAANDINTERMEDIATESPECTRALANALYSISHAVEIMPORTANTRESEARCHANDPRACTICALSIGNIFICANCEINTHEFIELDOFMICROBIALDEGRADATIONOFPESTICIDESANALYSISOFTHEINTERMEDIATEPRODUCTSISCONDUCIVETOAPROFOUNDUNDERSTANDINGOFTHEWHOLEPROCESSOFDEGRADATIONOFPESTICIDESFROMTHETHESISEXPERIMENT,THEEXPERIMENTALSTRAINSINTHEDEGRADATIONOFCHLORPYRIFOSCHLORPYRIFOSDEGRADATIONSTRONGITRATE7397ONTHE50MG/LOFCHLORPYRIFOSHIGHESTDEGRADATIONCONSTRUCTIONOFGENETICALLYENGINEEREDBACTERIACANPROVIDESTRAINRESOURCES,GOODRESEARCHVALUEANDAPPLICATIONPROSPECTTHROUGHONPH,SUBSTRATECONCENTRATIONANDOTHERENVIRONMENTALFACTORSMORETHANTHELEVELOFSINGLEFACTOREXPERIMENTS,THEDEGRADATIONOFSTRAINOBTAINEDIN30A28,PH75,SUBSTRATECONCENTRATIONOF50MGHASTHEBESTUNDERTHECONDITIONSOFDEGRADATIONTHESTRAINSWITHTHEREPORTEDDEGRADATIONOFCHLORPYRIFOSDEGRADATIONMECHANISMOFTHESAMEBIOLOGICALTHECHLORPYRIFOSISDEGRADATIONTOTCP,ANDTCPHASADIFFERENTDEGREEOFACCUMULATIONCHLORPYRIFOSCONTAMINATEDAREASINTHEMIXEDCULTUREOFCHLORPYRIFOSANDTCPDEGRADINGBACTERIADEGRADINGBACTERIAFORCONTROLINGCHLORPYRIFOSINENVIRONMENTALPOLLUTIONANDTOINORDERTOIMPROVEEFFICIENCYANDCONTROLOFENVIRONMENTALPOLLUTIONCONTROLEFFECTCHLORPYRIFOSWILLBECOMPLETELYDEGRADEDANDITWILLBENOPOLLUTION,NODAMAGE,NOTOXICCOMPOUNDSFORTHEENVIRONMENTANDTHELIVINGTHINGSALSOMUTATIONCANBESCREENEDBYHIGHEFFICIENCY,BROADSPECTRUMPESTICIDESSUCHASVARIATIONSINDEGRADATIONOFCHLORPYRIFOSDEGRADINGBACTERIATOENHANCEDEGRADATIONKEYWORDCHLORPYRIFOSDEGRADATIONINTERMEDIATESA29A30A31A32A33A34A35A36A37A38A39A40A41A41A41目录摘要A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42A42ABSTRACTA43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43目录A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43插图清单A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43表格清单A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43G269引言A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A431第1章绪论A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A43A432G20G20毒死蜱降解研究G13984G7235及研究意义A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A442G20G21毒死蜱研究G2469G4649G10628G10378A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A442G20G21G20毒死蜱的G10714G2282G5627G17148及毒G5627A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A442G20G21G21毒死蜱的应用G2469G4649G10628G10378A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A443G20G213毒死蜱降解的研究G2469G4649G10628A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A444第2章实验材料与方法A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A446G21G20实验药G2709G994G16809G2070A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A446G21G21实验G1214G3132G994G16786G7057A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A446G213实验方法G8505G20600A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A447G213G20G18209G2058毒死蜱降解菌的G3533G1871基A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A447G213G21G2058G3803G13543G1926G9354G9094A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A447G2133G2058G3803毒死蜱降解菌菌G5760G9094A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A447G213G23毒死蜱降解中间产物分析G1319G13007A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A447G2135毒死蜱的微生物降解G10317G5627分析G1319G13007A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A448第3章实验结论与分析A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4493G20毒死蜱G7643G1946G7366G13459的G13484G2058A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4493G21毒死蜱微生物降解G10317G5627分析研究A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4493G21G20G2033G3999G83G43G1552对毒死蜱降解的G5445G2721A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4493G21G21G3818G2164G11911源G8999G5242对毒死蜱降解的G5445G2721A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44103G213毒死蜱G2033G3999G8999G5242对毒死蜱降解的G5445G2721A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44103G21G23G6521种G18339对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G20G203G215G3533G1871G9213G5242对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G20G213G21G25G6683G5214G17728G17907对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G20G2133毒死蜱降解中间产物分析G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G203结果与讨论A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4415致谢A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4416参考文献A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A44A4417A45A46A47A48A49A50A51A52A53A54A55A56A57A51A58A59A60A61插图清单G328211毒死蜱G2282G4410G13479构G5347G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G1032G328231毒死蜱G7643G1946G7366G13459G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G1039G328232G2033G3999PHG1552对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G1039G328233G3818G2164G11911源G8999G5242对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10310G328234毒死蜱G2033G3999G8999G5242对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10311G328235G6521种G18339对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10311G328236G3533G1871G9213G5242对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10312G328237G6683G5214G17728G17907对毒死蜱降解的G5445G2721G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10312G32823824H降解的G7422G2164菌的对G10043G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10313G32823924H降解的G2164菌的G7691G2709G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10313G328231048H降解的G7422G2164菌的对G10043G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10313G328231148H降解的G2164菌的G7691G2709G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10314G328231272H降解的G7422G2164菌的对G10043G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10314G328231372H降解的G2164菌的G7691G2709G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10314G328241毒死蜱的降解过程G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G10315A62A63A64A65A66A67A68A69A70A71A72A73A74表格清单G1593211毒死蜱在G2520种G9354G2070中的G9354解G5242（25G263）G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G103G1032A62A63A64A65A66A67A68A69A70A71A72A73A75A76A75引言毒死蜱农药G1328为一种高G6940低毒的广谱G5627有机磷杀虫G2070，其为世界农业实G10628粮食增G2164G1209满足世界人口G993断增长的需求G1328G1998了重大的贡献。农药在世界范围的广泛使用G1075对世界环境产生了严重的污染。尤其，有机氯农药在环境中的残留和生物蓄G12227对G2520种生物和非生物目G7643G18129具有严重的潜在毒G5627。虽然毒死蜱类有机磷农药的降解G17907G5242比有机氯农药快，但是由于它们有能力抑G2058乙酰胆碱酯酶（ACHE）而具有急G5627毒G5627。乙酰胆碱酯酶可G1328为一种神经传递调节酶，可在交界处的突触降低乙酰胆碱的G8999G5242。当ACHE在突触灭活，例如G17902过一种有机磷农药，乙酰胆碱的G8999G5242在突触的G8999G5242仍然很高，肌肉或神经纤维持续G2469生刺激，最终导致衰竭和抽搐A77A78A79。毒死蜱的残留和降解一直是人们关注的问题。国内G3818科G4410家们对毒死蜱的降解G1328了许G3822的研究，生物降解尤其是微生物降解G1328为一类降解毒死蜱的有G6940方法日益成为科G4410家们关注和研究的焦点。对于毒死蜱的降解产物研究，ME1KLEG12573检测到了O乙基O3，5，6三氯2吡啶基硫逐磷酸酯和3，5，6三氯2吡啶酚TCP两种产物。CUSTAVOG12573检测到了O，O二乙基O3，5，6三氯2吡啶基磷酸酯，O乙基O3，5，6三氯2吡啶基硫逐助磷酸酯和TCP三种产物。WALIAG12573检测到了O,O二乙基O3，5，6三氯2吡啶基磷酸酯一种产物。申丽G12573检测到了O，O二乙基O3，5，6三氯2吡啶基磷酸酯，O，O二乙基O5，6二氯2毗陡基硫代磷酸酯，O，O二乙基O5氯2吡啶基硫代磷酸酯，O，O二乙基O2吡啶基磷酸酯，和TCP五种产物。JALAL检测到了二乙基磷酸酯，三乙基磷酸酯，O，O，O三乙基硫代磷酸酯，O，O二乙基O甲基硫代磷酸酯，二乙基3，5，6三氯2吡啶基硫逐磷酸酯，2，3，5三氯6O乙基吡啶，2，3，5三氯吡啶，和TCP七种产物。G1075有研究只检测到了TCP一种产物。本实验论文主要研究毒死蜱降解菌株对毒死蜱的降解G1209及对微生物降解毒死蜱的中间产物的分析。研究微生物对毒死蜱的降解G6940G7536，分析微生物降解毒死蜱产生的中间产物，有利于分析研究微生物降解毒死蜱的降解过程，从而掌握微生物降解毒死蜱过程中G2520种物G17148的G2476G2282，为G17839一G8505研究毒死蜱的微生物降解提供研究基础，为世界范围内解决毒死蜱残留和降解问题的新方法和措G7057提供可能。A80A81A82A83A84A85A86A87A88A89A90A91A92A86A93A94A95A96A95第1章绪论11毒死蜱降解研究背景及研究意义毒死蜱是一种广谱G5627有机磷杀虫G2070，是目G2081世界上杀虫G2070中销售额最大的G2709种，是陶氏G2282G4410公司于G209G255年在美国登记G6524广G5194G14731得G999利。G1075是目G2081G1852G10711应用最广泛的五种杀虫G2070G1055一。其G15999世界G2367生G13464G13467分成G2367生类和农用类两个类G2047，分G2047用G7481G1328G2367生杀虫G2070和农用杀虫G2070A97A98A99。毒死蜱于G20997年在G6117国G2474得了G8503G5347登记。本论文实验主要利用微生物对毒死蜱的降解G1328用，研究G7588种毒死蜱降解菌株对毒死蜱的降解G1328用G1209及对其G1194农药降解能力的分析，研究该菌株对毒死蜱降解的降解过程和降解谱，G5194对其降解中间产物G17839G15904分析。本实验有利于深刻了解和研究毒死蜱降解菌株对毒死蜱的降解G1328用及其降解过程，有利于毒死蜱降解过程中产物的研究，对毒死蜱降解菌株降解毒死蜱的过程研究及目G2081毒死蜱污染问题的解决具有深刻的研究意义和实践意义。12毒死蜱研究发展现状121毒死蜱的G10714G2282G5627G17148及毒G5627毒死蜱（CHLORPYRIFOS）,G2460G2529G1060G7043本G451G11345G15445G9177，其有G6940成分的G2282G4410G2529G12228为G50,G50G16二乙基G16G50G16G113,5,G25G16三氯G16G21G16吡啶基G12硫逐磷酸酯，G2282G4410G13479构G5347为G726A100A101A102A101A103A104A105A106A107A108A109A110G13443G2709毒死蜱为G11345G14406或G13785G9796G14406G20075G12902G10378G7242G1319，工业用毒死蜱为G9141G21656G14406G7242G1319，有微G5381的硫G18267G2631。毒死蜱G4658中G12573毒G5627杀虫G2070，G2419药大G21748急G5627经口LG39A111A112为G20G253G894/G895，急G5627经G11394LG39A111A112为G33G21G/G78G，对G16809验G2172物G11536G11567有G17743G5242刺激，对G11394G13944有G7138G7186刺激，长G7114间G3822G8437G6521触G1262产生G9808G1272。大G21748G1134急G5627经口G7092G1328用G2070G18339为003MG/G78G，G5942G5627经口G7092G1328用G2070G18339为0G20MG/G78G。G10411G5942G5627经口G7092G1328用G2070G18339为003MG/G78G。在G16809验G2070G18339G991G7422G16277致G11084G451致突G2476G451致G11296G1328用。G4472内G12366G8680中最高G1813许G8999G5242为（G55LG57）0G20MG/MA113。对G21072类及G8712生生物毒G5627较高，G15437G21171G21072LG38A111A112为G205MG/LG119G25G4579G7114，7G21G263G12。毒死蜱G4506G5242G2039G27（G2335G263），G10088点G23G205G16G2335G263G727G8785G2399为G215MG51G68（G215G263），在G8712中的G9354解G5242为G20G21MG/L,G9354于大G3822G6980的有机G9354G2070A97A113A99。G16826G16277G991G15932G726A114A115A116A117A118A119A120A121A122A123A124A125A122A123A126A125A127A128A129A130A131A132A133A132A132A132A134A135A136A137A132A138A139A132A137A139A132A140A141A134A132A136A141A132A129A142A143A144A127A144A120A125A127A128A120A127A119A134A145A134A145A134A146A126A125A143A128A129A130A131A136A141A147A137A147A132A132A147A132A132A147A132A132A148A149A150A149A151A152A153A154A155A156A157A158A159A160A157A161A162A163A164A165A166A167A168A169A170A171A172A173A174A175A176A177A178A179A180A181A180G20G21G21毒死蜱的应用G2469G4649G10628G10378毒死蜱于G209G255年G15999美国陶氏G2282G4410公司G20330G8437G5332G2469G1209G7481，其G1328为G1209G2081高毒杀虫G2070的G7379代G2709G5062经得到广泛的利用。毒死蜱对G7130虫具有触杀G451G13975毒和一G4462的G10083G14988G1328用,是一种广谱高G6940的杀虫G451杀G15740G2070。毒死蜱是一种神经毒G2070,能抑G2058乙酰胆碱酯酶的活G5627,使神经突触G18108G1313大G18339乙酰胆碱G12227G13870,致使突触G2530G14192G993断激活,神经纤维长G7411处于G1864G3871G10378G5589,G8503G5132的神经传导G2475G19471,从而使G7130虫中毒致死。毒死蜱在G7905株G2506G10267上的残留G7411G993长,但在G3315G3768中残留G7411较长。G20G21G21G20毒死蜱的应用G10628G10378毒死蜱广泛用于G19462G8847G15696G15512G451G15560G15744G451G3332G13781G15394G12573G3332G991G4487虫,G2955G19666可G19462G8847G7837G14469G451G7536G7653G451G15104G14768G451甘蔗G451G14469卉G12573G1328物上的G3822种G4487虫及G15740类G12573还可G1209G19462G8847蚊G451蝇G451跳蚤G451蜚蠊G12573G2367生G4487虫近年G7481在桑G7653上的应用逐渐增G3822,G5062在美国G451日本G451G2164拿大G451中国G1257310G3822个国家和G3332G2318注册,G2528G7114在非农业中G1075有所应用。毒死蜱是一种在麦类G451G8712稻G451玉米G451G7837G14469G451甘蔗G451G7536G7653G12573G3822种G1328物上普遍使用的高G6940广谱杀虫G2070,对G19462G8847G4579麦吸浆虫和G3332G991G4487虫G12573G18129有较好G6940G7536。G20G21G21G21毒死蜱G12573有机磷杀虫G2070的G10317点由于有机磷杀虫G2070的G2709种繁G3822，G5627能G1075千差万G2047，但G1328为杀虫G2070使用，它们G18129有一些G11468G2528的G10317点。有机磷杀虫G2070对G4487虫包括G15740虫G18129有较强的毒力，其一般在G8680G9213较高G7114，G15932G10628G1998较高的杀虫G6940力，而G1000G4487虫对有机磷的耐药G5627G2469G4649较为G13543G5942。有机磷类杀虫G2070一般对G1328物安G1852，易降解（分解成G7092毒物G17148），对环境污染G4579。在G7905物G1319内可代谢降解而解毒，在G7905物G1319G15932面可经日晒及