国际森林土壤研讨会课件Ibsen-Symposium on Forest Soils Presentation

1、Introduction :/greenmetropolis.ca/life/1332Oil sands exploration activities including mining and upgrading have exposed boreal forests in the surrounding area to elevated levels of N and S depositions from emissions of NOx and SO2 Negative effects of these depositions include soil acidification, N s

2、aturation, and eutrophication of water bodies Soil microbes in the boreal forest in northern Alberta have evolved in a relatively static ecosystem that is historically known to be N limitingSoil microbes are important because they exert control over the soil C and N cycles and influence the resilien

3、ce of an ecosystemThere is limited information on how specific microbial groups are affected :/modernfarmer /2021/04/microbes-will-feed-world-real-farmers-grow-soil-crops/1.The overall diversity of microbial species will decrease after 9 years of simulated N and S deposition.2.The microbial species

4、community composition will be altered after 9 years of simulated N and S deposition. HypothesisLocated approximately 100km southeast of the city of Fort McMurray, Alberta, CanadaThe dominating tree species are trembling aspen (Populus tremuloides) and white spruce (Picea glauca) and based on the Can

5、adian system of soil classification the soils in the sites are classified as mainly Gray Luvisols N addition (30 kg N ha-1 yr-1 as NH4NO3), S addition ( 30 kg S ha-1 yr-1 as Na2SO4)Study Site Soil samples were taken at 0-15cm from three randomly selected points in each plotSoil DNA was extracted wit

6、h the SoilMasterTM DNA Extraction Kit PCR was conducted using bacterial and archaeal primers that target the 16S ribosomal DNAQuantitative Insights into Microbial Ecology (QIIME) used for microbiome analysisOperational taxonomic units (OTUs) were selectedMaterials and MethodsResultsTreatmentpHSoil M

7、oistureTotal carbon (g kg-1)Total nitrogen(g kg-1)CK 5.0716.4% 6.820.28N4.8315.4%6.860.36S4.9415.6%6.940.54NS4.7114.6%7.120.50No significant differencesDiscussion Our results showed that the soil microbial community diversity and composition were not significantly affected by 9 years of N and/or S d

8、eposition. WHY? Potential explanations for the recorded results: levels of N and S deposition usedDiscussion Our results showed that the soil microbial community diversity and composition were not significantly affected by 9 years of N and/or S deposition. WHY? Potential explanations for the recorde

9、d results: levels of N and S deposition used length of the experiment 2021199519851990198020002005Fierer et al. 2021 Yao et al. 2021DeForest et al. 2004This study30kg ha-1 yr-117.5 - 280 kg ha-1 yr-134- 291 kg ha-1 yr-1 Even though N and/or S deposition did not cause significant changes there are so

10、me general trends that should be considered, including:The copiotrophic hypothesis N addition increased fast growing Proteobacteria Control showed higher number of slow growing AcidobacteriaConclusionsAcknowledgements I would like to thank my supervisor Dr. Scott Chang and Dr. Bin Ma for their guida

11、nce and assistance on this experiment.I would like to acknowledge the Land Reclamation International Graduate School and NSERC Create for financial and logistical support.Lastly, I would like to acknowledge and thank the University of Alberta and all of my colleagues. ReferencesCaporaso JG, Kuczynsk

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