CdTe-CdS 量子点在Nafion-GNP膜上的电化学发光及其应用于肌红蛋白的高灵敏检测

1、CdTe/CdS 量子点在Nafion/GNP 膜上的电化学发光及其应用于肌红蛋白的高灵敏检测王国涛, 赵倩, 贾丽萍, 潘吉超, 任腾飞, 王怀生*聊城大学化学化工学院 山东聊城 252059( 通信联系人,电话/传真:Email: hswang 肌红蛋白(Mb 1 由153个氨基酸残基组成的多肽链和一个血红素(铁卟啉所构成,它主要是通过可逆性地与氧结合和释放,为肌肉组织储存和转运氧。由于Mb 是一个较小分子的球蛋白,心肌或骨骼肌损伤时释放出的Mb 可以从肌肉组织漏到循环血中去,最后经肾脏代谢和清除从尿中排出,形成高肌红蛋白血症和肌红蛋白尿。它在诊断急性冠状动脉综

2、合症、心血管疾患以及急性心肌缺血致死等疾病时,较其他生化标志物呈阳性结果早。因此,建立快速、灵敏的检测肌红蛋白含量的分析方法具有重要的临床意义。 利用纳米金 (GNP 和Nafion 将CdTe/CdS 量子点 (QDs 修饰到玻碳电极上,以三丙胺为共反应物 3,研究了量子点在该复合膜修饰电极上的电化学发光行为。结果表明,复合膜内的量子点的电化学发光强度和稳定性都要远远好于分散在溶液中的量子点在裸电极上的电化学发光行为 4 (如图 1 所示。在 pH 为8.0的磷酸盐缓冲溶液 (PBS 中,基于肌红蛋白 (Mb 能有效地猝灭量子点的电化学发光 (ECL 强度,建立了一种灵敏度较高的检测Mb 的

3、新方法,同时我们探讨了Mb 猝灭QDs 电化学发光的可能机理。Mb 浓度在10 ng/Ml-180 ng/mL 范围内,分析信号Log(I 0-I/I 与肌红蛋白浓度的对数LogC Mb 有良好的线性关系,检出限为0.020 ng/mL (S/N = 3。 10002000300040000.00.20.40.60.8 1.0 1.2-40-30-20-10010cb aAE C L i n t e n s i t y (a .u Potential/V a b cBC u r r e n t /µ-50510152025303575150225300375E C L i n t e

4、 n s i t y /a .u .t/s图1量子点在裸电极(a 和在Nafion 膜(b Nafion/GNP 膜(c 上的循环伏安曲线和电化学发光曲线.图2 Mb 对Nafion/GNP 膜内CdTe/CdS 量子点电化学发光的猝灭曲线. 插图为相应的线性关系曲线. 感谢国家自然科学基金(Nos. 20775031, 21075059的资助。参考文献:1 A.H. Liu, M.D. Wei, I. Honma, H.S. Zhou, Anal. Chem. 77 (2005 8068.2 J.J. Miao, T.Ren, L. Dong, J.J. Zhu, H.Y. Chen, Sm

5、all 1 (2005 802.3 Y.L. Mei, H.S. Wang, Y.F. Li, Z.Y. Pan, Electroanalysis 22 (2010 155.4 L.J. Hua, H.Y. Han, H.B. Chen, Electrochimica Acta 54 (2009 1389. Electrochemiluminescent behavior of CdTe/CdS quantum dots co-immobilized on electrode surface with gold nanoparticles and its sensing application

6、 to myoglobinGuo-Tao Wang, Qian Zhao, Li-Ping Jia, Ji-Chao Pan, Teng-Fei Ren, Huai-Sheng Wang College of Chemistry and Chemical Engineering, Liaocheng University, 252059The anodic electrochemluminescent behavior of 3-mercaptopropionic acid (MPA capped CdTe/CdS quantum dots (QDs co-immobilized on gla

7、ssy carbon electrode (GCE with gold nanoparticles (GNP and its sensing application to myoglobin were studied in this paper. The results indicated that the CdTe/CdS could be co-immobilized with GNP firmly on the electrode surface using Nafion as the supporting film. Compared with the electrochemilumi

8、nescence (ECL intensity of the QDs dispersed in aqueous solution at the bare GCE, the ECL intensity and stability of the QDs co-modified with GNP on GCE were greatly enhanced. The ECL sensing application to myoglobin (Mb was proposed based on its quenching to the ECL intensity of CdTe/CdS QDs in pH 8.0 phosphate buffer solution (PBS with tri-n-propylamine (TPrA as coreactant. Under the optimal conditions, the ECL intensity was decreased with the increase of Mb concentration in the range of 1.0-120 ng/mL with the d